In Silico Expression Analysis
2016
Bolívar, Julio | Hehl, Reinhard | Bülow, Lorenz
Information on the specificity of cis-sequences enables the design of functional synthetic plant promoters that are responsive to specific stresses. Potential cis-sequences may be experimentally tested, however, correlation of genomic sequence with gene expression data enables an in silico expression analysis approach to bioinformatically assess the stress specificity of candidate cis-sequences prior to experimental verification. The present chapter demonstrates an example for the in silico validation of a potential cis-regulatory sequence responsive to cold stress. The described online tool can be applied for the bioinformatic assessment of cis-sequences responsive to most abiotic and biotic stresses of plants. Furthermore, a method is presented based on a reverted in silico expression analysis approach that predicts highly specific potentially functional cis-regulatory elements for a given stress.
اظهر المزيد [+] اقل [-]المعلومات البيبليوغرافية
الناشر Springer
الرقم التسلسلي المعياري الدولي (ردمد) 4939-6396 | 4939-6394 | 4939-6396تم تزويد هذا السجل من قبل Julius Kühn-Institut