Susceptibility of North American big brown bats (Eptesicus fuscus) to infection with European bat lyssavirus type 1
2008
Franka, R. | Johnson, N. | Müller, Thomas | Vos, A. | Neubert, L. | Freuling, Conrad Martin | Rupprecht, C.E. | Fooks, A.R.
The aim of this study was to determine the susceptibility of insectivorous bats (using the big brown bat as a model) to infection with European bat lyssavirus type 1 a (EBLV-1 a), to assess the dynamics of host immune responses and to evaluate the opportunity for horizontal viral transmission within colonies. Two isolates of EBLV-1 a, originating from Slovakia (EBLV-1 aSK) and Germany (EBLV-1 aGE), were tested. Four different routes of inoculation were used with isolate EBLV-1aSK [104,8 mouse intracerebral median lethal dose (MICLD50) in 50 pl]: intramuscular (i.m.) in the deltoid area or masseter region, per os (p.o.) and intradermal (i.d.) scratches. Isolate E13LV-1aGE (103-2 and 102.2 MICLD50 in 20 pl) was inoculated via the intranasal (i.n.), i.m. (low- and high-dose groups, into pectoral muscles); p.o. and intracerebral (i.c.) routes. None of the bats infected by the i.n., p.o. or i.d. route with either virus isolate developed disease during the experiments (91 or 120 days, respectively). Incubation periods were 9-12 days for i.c.-inoculated bats (66 % mortality), 12-33 days for bats inoculated i.m. with the higher dose (23-50 % mortality) and 21 -58 days in bats inoculated i.m. with the lower dose of virus (57 % mortality). Virus or viral RNA in bat saliva was detected occasionally, as early as 37 days before death. All i.d.-inoculated and the majority of i.m.-inoculated bats seroconverted within 7-10 days of inoculation. These observations suggest that exposure of bats to varying doses of EBLV-11 from rabid conspecifics via natural (i.d.) routes could lead to an abortive infection and serve as a natural mode of immunization resulting in the presence of virusneutralizing antibodies in free-ranging bats
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
الناشر Microbiology Society (früher: Society for General Microbiology (SGM))
الرقم التسلسلي المعياري الدولي (ردمد) 0022-1317 | 1465-2099تم تزويد هذا السجل من قبل Friedrich-Loeffler-Institut
