Evaluation of a real-time PCR assay to detect Coxiella burnetii
2006
Klee, S. R. | Ellerbrok, H. | Tyczka, J. | Franz, T. | Appel, B.
ألمانية. We evaluated real-time PCR assays for the detection of C. burnetii which targets sequences that are present either in one (icd) or in several copies (transposase of IS1111a) on the chromosome. The assays are highly sensitive, with reproducible detection limits of approximately 10 copies per reaction, at least 100 times more sensitive than capture ELISA, when performed on infected placenta material and specific for C. burnetii. The numbers of IS1111 elements in the genomes of 75 C. burnetii isolates were quantified by real-time PCR and proved to be highly variable
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
الناشر Blackwell
الرقم التسلسلي المعياري الدولي (ردمد) 0077-8923 | 1749-6632تم تزويد هذا السجل من قبل German Federal Institute for Risk Assessment