Differential Antimicrobial Potential of Ajuga integrifolia Buch. Ham. Ex D.Don Based on Extraction Solvents
2025
Wajid Khan | Nisar Ali | Muhammad Nazir Uddin | Dawood Ghafoor | Muhammad Ishaq | Haji Khan | Muhammad Rizwan | Shahab Khan
The current study aimed to evaluate the effect of extraction solvents on the anti-bacterial and anti-fungal efficacy of the stem and roots of Ajuga integrifolia Buch. Ham. ex D. Extracts were prepared in different solvents and tested against fungi and bacteria species including, Agrobacterium tumefaciens, Xanthomonos oryzae, Citrobacter freundi, Alternaria alternata, A. solani and Aspergillus niger. Antibacterial efficacy of the Ajuga integrifolia was carried out by disc diffusion susceptibility method and antifungal efficacy by well diffusion susceptibility method. Methanol stem extract revealed efficacy against C. freundi by producing a 63% zone of inhibition at 3000µg/disc while methanol roots extract produced 77% ZI against A. tumifaciens. Methanol root and stem extracts produced an equal zone of inhibition (97%) at 1000 µg/ml concentration against A. alternata and A. solani. The results of the study clearly stated that the polarity of the solvents used in the extraction procedure affects the bioactivities of the extracts.
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