Alternative splicing of coq-2 controls the levels of rhodoquinone in animals
2020
Tan, June H. | Lautens, Margot | Romanelli-Cedrez, Laura | Wang, Jianbin | Schertzberg, Michael R. | Reinl, Samantha R. | Davis, Richard E. | Shepherd, Jennifer N. | Fraser, Andrew G. | Salinas, Gustavo
Parasitic helminths use two benzoquinones as electron carriers in the electron transport chain. In normoxia, they use ubiquinone (UQ), but in anaerobic conditions inside the host, they require rhodoquinone (RQ) and greatly increase RQ levels. We previously showed the switch from UQ to RQ synthesis is driven by a change of substrates by the polyprenyltransferase COQ-2 (Del Borrello et al., 2019; Roberts Buceta et al., 2019); however, the mechanism of substrate selection is not known. Here, we show helminths synthesize two coq-2 splice forms, coq-2a and coq-2e, and the coq-2e-specific exon is only found in species that synthesize RQ. We show that in Caenorhabditis elegans COQ-2e is required for efficient RQ synthesis and survival in cyanide. Importantly, parasites switch from COQ-2a to COQ-2e as they transit into anaerobic environments. We conclude helminths switch from UQ to RQ synthesis principally via changes in the alternative splicing of coq-2.
اظهر المزيد [+] اقل [-]Agencia Nacional de Investigación e Innovación
اظهر المزيد [+] اقل [-]Canadian Institutes of Health Research
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Agencia Nacional de Investigación e Innovación
