Syzygium aromaticum (clove) and Thymus zygis (thyme) essential oils increase susceptibility to colistin in the nosocomial pathogens Acinetobacter baumannii and Klebsiella pneumoniae
2020
Vázquez-Ucha, Juan, C | Martínez-Guitián, Marta | Lasarte-Monterrubio, Cristina | Conde-Pérez, Kelly | Arca-Suárez, Jorge | Álvarez-Fraga, Laura | Pérez, Astrid | Crecente-Campo, José | Alonso, María, J | Bou, Germán | Poza, Margarita | Beceiro, Alejandro | Instituto de Investigación Biomédica de A Coruña [La Corogne, Espagne] (INIBIC) ; A Coruña University Hospital [La Corogne, Espagne] | Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Molecular Medicine and Chronic Diseases Research Centre [Santiago de Compostela, Spain] (CIMUS) ; Universidade de Santiago de Compostela [España] = University of Santiago de Compostela [Spain] = Université de Saint-Jacques-de-Compostelle [Espagne] (USC) | This study was funded by projects IN607A 2016/22 (GAIN), PI15/00860, PI18/00501 and REIPI RD16/0016/006 (co-financed by the European Development Regional Fund "A way to achieve Europe" and the operative Intelligent Growth program 2014-2020) awarded to GB, PI14/00059 and PI17/01482 awarded to AB and MP (Instituto de Salud Carlos III). JCVU was financially supported the pFIS Programme (FI18/00315, ISCIII), MMG by a Clara Roy grant (SEIMC), KCP by IN607A 2016/22 (GAIN), CLM by IN606A-2019/029 (GAIN) and JAS by the Rio Hortega program (CM19/00219, ISCIII) and the FPNS (post-specialization grant).
International audience
اظهر المزيد [+] اقل [-]إنجليزي. The discovery of new antibiotics that are effective against Acinetobacter baumannii and Enterobacteralesis a research priority. Several essential oils (EOs) have displayed some antimicrobial activity and could potentially act as antibiotic adjuvants. Research in this area aims to develop new therapeutic alternatives to treat infections caused by these pathogens.MICs of different EOs were determined against A. baumannii and Klebsiella pneumoniae. Combined disk diffusion tests and checkerboard assays were used to study the synergy between the EOs and antibiotics. The fractional inhibitory concentration index (FIC index ) was calculated in order to categorize the interaction. Timekill assays were also performed.The EOs that displayed the highest levels of antimicrobial activity were clove (Syzygium aromaticum L.) and thyme (Thymus zygis L.). Combined disk diffusion tests and checkerboard assays revealed synergy between these EOs and colistin. Addition of either clove or thyme EO decreased the MIC of colistin by 8-to 64-fold and 8-to 128-fold in the colistin-resistant A. baumannii and K. pneumoniae strains, respectively (FIC index ≤ 0.5, synergy). MICs were also reduced in the colistin-susceptible strains. Time-kill assays also indicated the strong activity of the combined therapy. In summary, the use of clove or thyme EO in combination with colistin could improve the efficacy of the antibiotic and significantly reduce the concentrations needed to inhibit growth of A. baumannii and K. pneumoniae.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique