Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes
2014
Silipo, Alba | Vitiello, Giuseppe | Gully, Djamel | Sturiale, Luisa | Chaintreuil, Clémence | Fardoux, Joel | Gargani, Daniel | Lee, Hae-In | Kulkarni, Gargi | Busset, Nicolas | Marchetti, Roberta | Palmigiano, Angelo | Moll, Herman | Engel, Regina | Lanzetta, Rosa | Paduano, Luigi | Parrilli, Michelangelo | Chang, Woo-Suk | Holst, Otto | Newman, Dianne K. | Garozzo, Domenico | d'Errico, Gerardino | Giraud, Eric | Molinaro, Antonio | Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant’Angelo ; University of Naples Federico II = Università degli studi di Napoli Federico II | Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR) | Biologie et Génétique des interactions Plantes-parasites pour la Protection Intégrée ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | University of Texas at Arlington ; Partenaires INRAE | Division of Biology and Biological Engineering ; Howard Hughes Medical Institute (HHMI) | Howard Hughes Medical Institute (HHMI) | Leibniz-Center for Medicine and Biosciences ; Partenaires INRAE | Chonbuk National University | French national research agency ANR-SESAM-2010-BLAN-170801 ANR-BugsInaCell-13-BSV7-0013-02 ; Italian Ministry of Education, Universities and Research 2009J98Z3_001 BJ23MN_007 L9SH3K RBNE08HWLZ ; Mizutani Foundation for Glycoscience ; NASA NNX12AD93G ; Howard Hughes Medical Institute | ANR-13-BSV7-0013,BugsInACell,Accommodation intracellulaire des bactéries symbiotiques fixatrices d'azote(2013)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Lipopolysaccharides (LPSs) are major components of the outer membrane of Gram-negative bacteria and are essential for their growth and survival. They act as a structural barrier and play an important role in the interaction with eukaryotic hosts. Here we demonstrate that a photosynthetic Bradyrhizobium strain, symbiont of Aeschynomene legumes, synthesizes a unique LPS bearing a hopanoid covalently attached to lipid A. Biophysical analyses of reconstituted liposomes indicate that this hopanoid-lipid A structure reinforces the stability and rigidity of the outer membrane. In addition, the bacterium produces other hopanoid molecules not linked to LPS. A hopanoid-deficient strain, lacking a squalene hopene cyclase, displays increased sensitivity to stressful conditions and reduced ability to survive intra-cellularly in the host plant. This unusual combination of hopanoid and LPS molecules may represent an adaptation to optimize bacterial survival in both free-living and symbiotic states.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique