GC-MS analysis and cytotoxic activity of the n-hexane fraction from Curcuma sahuynhensis Škornick. & N.S.Lý leaves collected in Vietnam

Tran, Van Chen; Truong, Minh-Nhut; Tran, Thi Thuy Quynh; Nguyen, Thanh To Nhi; Nguyen, Hoang Khanh Linh

GC-MS analysis and cytotoxic activity of the n-hexane fraction from Curcuma sahuynhensis Škornick. & N.S.Lý leaves collected in Vietnam

2024

Tran, Van Chen | Truong, Minh-Nhut | Tran, Thi Thuy Quynh | Nguyen, Thanh To Nhi | Nguyen, Hoang Khanh Linh

Curcuma sahuynhensis Škornick. & N.S.Lý is an endemic plant in Vietnam that has been used by the Sa Huynh people as a spice and medicine to cure illnesses linked to digestive disorders. Very little information is available so far about the chemical composition and biological effects of C. sahuynhensis. To find new pharmaceutical ingredients, the in vitro cytotoxic effect and the chemical profile of C. sahuynhensis leaf extract were investigated. In this study, the percolation method and liquid-liquid dispersion technique were used to extract dry sample powder. The chemical composition was detected by gas chromatography-mass spectrometry (GC-MS). The Sulforhodamine B and MTT methods were used to determine the cytotoxic activity. The chemical composition analysis showed that the leaf extract contained 14 components. The major components in the n-hexane extract were 6,10,14-trimethylpentadecan-2-one, phytol, 1-ethylbutyl hydroperoxide, isoborneol, 1-methylpentyl hydroperoxide, and neophytadiene. On human cancer cell lines, namely MFC-7, SK-LU-1, Hela, MKN-7, and HL-60, the leaf extract showed dose-dependent cytotoxic activity, with IC50 values ranging from 221.70±10.24 to 369.42±10.60 ?g/mL. The present study provides significant information on the chemical components and cytotoxic effects of the n-hexane extract from C. sahuynhensis leaves. The findings will continue to be crucial in future research on the evaluation of secondary metabolite compound analysis for cancer therapeutic effects.

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