Characterization of two Winter wheat varieties' responses to freezing in a frigid region of China

Cold stress is one of the main abiotic stresses faced by winter wheat, which results in significant yield loss, especially in the harsh winter of the Heilongjiang province. Glycine betaine (GB), an important osmolyte in higher plants, helps in stabilization of the plasma membrane and its protection from cold stress. In the present study, two winter wheat varieties, Dongnongdongmai1 (DM1) and Jimai22 (J22), which differ in their cold-resistance were planted under natural conditions and used for analyzing the relative electrical conductivity, malondialdehyde (MDA) content, betaine aldehyde dehydrogenase (BADH) activity, expression of BADH, and the content of GB. The cold-resistant variety, DM1, showed a greater increase in BADH activity and GB content and decreased MDA content than J22 under freezing conditions. GB was observed to have an obvious role in inhibiting the MDA content. This was reflected by the expression of BADH and enhanced tolerance to cold stress upon GB accumulation, which helped in membrane stabilization. The results of the present study confirmed the role of GB in conferring cold-resistance in the DM1 winter cultivar and could benefit other studies aimed at improving the tolerance of other wheat cultivars as well as other crops.

The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author.