Germplasm Selection and Comprehensive Evaluation of Maize Inbred Lines at Germination and Seedling Stage for Saline–Alkali Tolerance

Soil salinization, recognized globally as a significant environmental issue, can directly impact the sustainable development of agriculture worldwide. As a key crop driving the global agricultural economy, maize is particularly vulnerable to the detrimental effects of salt–alkali stress, which can impede its growth and development from germination through the seedling stages. In this study, 32 maize inbred lines were screened for saline–alkali resistance identification. We performed four treatments—200 mmol/L NaCl, 100 mmol/L Na<sub data-eusoft-scrollable-element="1">2</sub>CO<sub data-eusoft-scrollable-element="1">3</sub>, 200 mmol/L NaCl with 25 mmol/L Na<sub data-eusoft-scrollable-element="1">2</sub>CO<sub data-eusoft-scrollable-element="1">3</sub>—and distilled water (CK) served as a control with a complete randomized design. Principal component analysis and the membership function value method were employed to evaluate the salt–alkali tolerance of these lines. The results indicate complex correlations among various traits at different growth stages. The relative correlation of each index was established by measuring the key physiological indexes (germination rate, root length and seedling height) of different maize inbred lines at the germination stage and seedling stage and standardizing them via the membership function value method. Subsequently, the principal component analysis method was used to reduce the dimensions of the standardized data, and the main factors affecting maize germination and seedling growth were extracted. The salt–alkali tolerance of maize inbred lines was comprehensively evaluated, and the salt–alkali tolerance and sensitive materials of maize germination and seedling stage were identified. This study found that the coefficient of variation of each trait is relatively rich. Therefore, in the preliminary screening of breeding germplasm resources, different reference indexes can be selected according to different periods and stress conditions. The enzyme activity results revealed significant variations in enzyme activity across different treatments and materials, with changes in superoxide dismutase (SOD) and peroxidase (POD) exhibiting relative stability, thereby reflecting the physiological response mechanisms of maize under salt–alkali stress. In this study, through principal component analysis, it was found that the germ and germination factors at the germination stage of maize and the root weight, root length, and seedling development factors at the seedling stage affected its saline–alkali resistance. The root weight factor, seedling development factor, and root length factor may affect the growth and development of maize at the seedling stage. Through the comprehensive analysis of the data of the germination stage and seedling stage, it can be seen that the materials with better salt resistance are Zm4 and Zm32; the material with better alkali resistance is Zm30; the material with better salt and alkali resistance is Zm2. Through the application of comprehensive evaluation system, researchers can more effectively screen out maize germplasm resources with excellent genetic characteristics to promote maize variety improvement and genetic diversity protection.