Cinnamyl alcohol dehydrogenase downregulation in poplar wood increases saccharification after dilute acid pretreatment: a key role for lignin revealed by a multimodal investigation
2025
du Pasquier, Julien | Zoghlami, Aya | Naudin, Youri | Déjardin, Annabelle | Pilate, Gilles | Paës, Gabriel | Perre, Patrick | Fractionnement des AgroRessources et Environnement (FARE) ; Université de Reims Champagne-Ardenne (URCA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Centre Européen de Biotechnologies et Bioéconomie (CEBB) | CentraleSupélec | Laboratoire de Génie des Procédés et Matériaux (LGPM) ; CentraleSupélec-Université Paris-Saclay | Biologie intégrée pour la valorisation de la diversité des Arbres et de la Forêt (BioForA) ; Office national des forêts (ONF)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Université Paris-Saclay
International audience
اظهر المزيد [+] اقل [-]إنجليزي. This study is the first to apply dilute acid pretreatment (DAP) under different severity conditions to poplar wood genetically modified for the cinnamyl alcohol dehydrogenase (CAD1) gene, which is involved in the lignin biosynthesis pathway. The carefully selected pretreatment conditions resulted in glucose yields that were 15 points higher for the hpCAD poplar line than for the wild-type (WT) wood after 48 h of enzymatic hydrolysis. To explain this higher saccharification rate, the chemical, spectral and structural changes in WT and hpCAD wood were analyzed in relation to the severity of the pretreatment process. Although few differences were found at the chemical level, variations in autofluorescence and cell deformation were more significant: at high severity, the cells of hpCAD wood observed by nanotomography were more easily deformed, but their middle lamella was more resistant than those of WT wood. All these differences are possibly explained by changes in the molecular structure of lignin in hpCAD wood, leading to the formation of more hydrophobic shorter monomer chains with fewer lignin‒carbohydrate interactions.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique