PCR detection of Non-Tuberculous Mycobacteria 16s rDNA and its Relation to Bovine IL6 Concentration in Subclinical Mastitis Cow's Milk

Rawa Zubairi; Moaed H. Sayhood

PCR detection of Non-Tuberculous Mycobacteria 16s rDNA and its Relation to Bovine IL6 Concentration in Subclinical Mastitis Cow's Milk

2025

Rawa Zubairi | Moaed H. Sayhood

The mammary tissue gets inflamed when it is affected by mastitis. Inflammation is indicated by changes in the appearance and color of clots, also by an increase in milk cell counts. The current research sought to determine how leukocytes alter Interleukin (IL) 6 concentrations in milk and the role of nontuberculous mycobacteria concentration (NTM) as an accusatory agent in subclinical mastitis (SCM). The major screening method for subclinical mastitis in samples of cow's milk was Modified Whiteside Test (MWT). A total of 58 out of 70 (82.9%) exhibited positive SCM reactions, according to the test results; of these, 22 out of 70 (31.5%) had a strong SCM reaction (+++ve), 25 (35.7%) had a slight (1.5%) SCM reaction (++ve), and 11 (15.7) had a moderate SCM reaction (++ve). The 16s rRNA-based polymerase chain reaction (PCR) has been used for confirming the existence of NTM spp in the examined cow's milk samples. Out of 70 milk samples, 3 (4.3%) had positive PCR results. ELISA test was used to ascertain the impact of somatic cell count on concentration of IL-6 in cow's milk. The test's findings have shown that there was a significant variation (P<0.05) in the mean concentration regarding IL-6 in SCM milk. Cow's milk samples with considerable SCM reaction (++ve) had a higher mean IL-6 concentration (474.5670), while milk samples with modest SCM reaction (+ve) had a lower mean IL-6 concentration.

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