THE ROLE OF GENISTEIN AND FISH OIL IN MOLECULAR AND BIOCHEMICAL DISORDERS OF OSTEOCLASTOGENESIS AS A RISK FACTOR FOR OSTEOPOROSIS INDUCED BY ANASTROZOLE IN LABORATORY RATS (Rattus norvegicus)

In order to determine the toxic effects of anastrazol and its relationship to the incidence of osteoporosis, this study was conducted (56 females laboratory rats ) aged (3-4 month) divided into (7 groups) each group composed 8 female rats as follows: The first group was treated with a anastrazol (0.02 mg/1 kg body weight) for a 60 days. The second group was treated with anstrazol (0.02 mg/1 kg body weight) and fish oil (3750 mg/1 kg of body weight) for 60 days. The third group was treated with anstrazol (0.02 mg/1 kg body weight), and phytoestrogen (genstien) (20 mg/1 kg of body weight) for 60 days. The fourth group was treated with anstrazol (0.02 mg/1 kg body weight) , fish oil (3750 mg/1 kg of body weight)  and phytoestrogen (genstien) (20 mg/1 kg of body weight) for a 60 Day. The fifth group is treated with a drug phytoestrogen (genstien) (20 mg/1 kg of body weight) for a 60-day. The sixth group were treated with fish oil (3750 mg/1 kg of body weight) for a 60-day. The seventh group is a group control are not treated by any drugs for a 60 days. The biochemical results showed a significant decline in value of estrogen in the GI (43.925±2.895) compared with GII, GIII and GIV while significant improvement observed in the estrogen level of GII, GIII and GIV respectively (70.308±2.140), (67.42±3.812) and (74.15± 3.603) compared with the first group (43.925±2.895). Also  the GIV showed the optimal improvement in estrogen level (74.15±3.603), as well, the biochemical results showed significant increase in the level of the alkaline phosphatase in the GI which showed (190.02±3.98) compared with GII, GIII and GIV while showed significant improvement of alkaline phosphatase values in the GII, GIII and GIV respectively (180.57±1. 89), (181.37±5. 03), (176. 8±2, 41).In Real-Time PCR RANK in the first group showed up-regulation (12.157±1.59) compared with the second, third and fourth groups (5.50±90.601), (6.903±0.130) and (3.018±0.027) respectively. The results also showed an up-regulation RANKL in Group I (8.458±0.766) compared with the second, third and fourth groups respectively (6.071±0.53), (4.970±0.228) and (3.398±0.114). While the results showed an up-regulation OPG in the second, third and fourth respectively (1.924±0.08), (1.014±0.064) and (5.567±1.419) compared in the first group (0.009±0.0076).We concluded that the derivatives like genestein and fish oil lead to ameliorate the toxic effect of anastrazole, also these derivatives may minimized the risk of osteoporosis caused by estrogen deficiency