THE SUITABLE ANTICOAGULANT, TIME AND TEMPERATURE FOR BLOOD SAMPLE COLLECTION

Adel M. Al zobidy; Dhuha Adel Kareem; Fawzi Alasadi

THE SUITABLE ANTICOAGULANT, TIME AND TEMPERATURE FOR BLOOD SAMPLE COLLECTION

2020

Adel M. Al zobidy | Dhuha Adel Kareem | Fawzi Alasadi

The blood is a connective tissue that composes of suspended cells in liquidmatrix. In this study ,we discuss the effect of three factors on blood sampling duringthe collection of blood samples (anti-coagulants, temperature and duration of storage). The delay of storage processing led to hemolysis of blood sample , increase ofhemoglobin, decrease of red cells ,triglycerides, hematocrite, mean corpuscularvolume, mean corpuscular hemoglobin and its concentration and increase number ofwhite blood cells. The histological analysis of blood sample during long-term storageshowed a slight difference in the shape and size of RBC,WBC and platelets as well ascondensed nucleus bluish cytoplasm and basophilic segmented nucleus in neutrophilsin addition to decrease in sodium ,calcium and chloride. The effect of temperature onblood sampling showed slight variation in shape and size of RBC , basophilicneutrophils and platelets aggregation and decrease of leukocyte number and K levels .

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