Start-up phase of an anaerobic full-scale farm reactor – Appearance of mesophilic anaerobic conditions and establishment of the methanogenic microbial community
2016
Goux, Xavier | Calusinska, Magdalena | Fossépré, Marie | Benizri, E | Delfosse, Philippe | Laboratoire Sols et Environnement (LSE) ; Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) | Luxembourg Institute of Science and Technology (LIST)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. The goal of this study was to investigate how the microbial community structure establishes during the start-up phase of a full-scale farm anaerobic reactor inoculated with stale and cold cattle slurry. The 16S/18S high-throughput amplicon sequencing results showed an increase of the bacterial, archaeal and eukaryotic diversity, evenness and richness during the settlement of the mesophilic anaerobic conditions. When a steady performing digestion process was reached, the microbial diversity, evenness and richness decreased, indicating the establishment of a few dominant microbial populations, best adapted to biogas production. Interestingly, among the environmental parameters, the temperature, alkalinity, free-NH3, total solids and O2 content were found to be the main drivers of microbial dynamics. Interactions between eukaryotes, characterized by a high number of unknown organisms, and the bacterial and archaeal communities were also evidenced, suggesting that eukaryotes might play important roles in the anaerobic digestion process.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique