Parental Selenium Nutrition Affects the One-Carbon Metabolism and the Hepatic DNA Methylation Pattern of Rainbow Trout (Oncorhynchus mykiss) in the Progeny
2020
Wischhusen, Pauline | Saito, Takaya | Heraud, Cécile | Kaushik, Sadasivam | Fauconneau, Benoit | Antony Jesu Prabhu, Philip | Fontagné-Dicharry, Stéphanie | Skjaerven, Kaja | Nutrition, Métabolisme, Aquaculture (NuMéA) ; Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Institute of Marine Research [Bergen] (IMR) ; University of Bergen (UiB) | I-SITE E2S: ENERGY AND ENVIRONMENTAL SOLUTIONS from theUNIVERSITY OF PAU AND PAYS ADOUR, UPPA (contract number 2017-17 and contract number 2018–178), | INSTITUTE OF MARINE RESEARCH (ParSel project number 15329), | ADISSEO FRANCE SAS (SelGen contract number 22001062 labelled by the Institut Carnot France Futur Elevage IC-F2E). | ANR-16-IDEX-0002,E2S,E2S(2016)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Selenium is an essential micronutrient and its metabolism is closely linked to the methionine cycle and transsulfuration pathway. The present study evaluated the effect of two different selenium supplements in the diet of rainbow trout (Onchorhynchus mykiss) broodstock on the one-carbon metabolism and the hepatic DNA methylation pattern in the progeny. Offspring of three parental groups of rainbow trout, fed either a control diet (NC, basal Se level: 0.3 mg/kg) or a diet supplemented with sodium selenite (SS, 0.8 mg Se/kg) or hydroxy-selenomethionine (SO, 0.7 mg Se/kg), were collected at swim-up fry stage. Our findings suggest that parental selenium nutrition impacted the methionine cycle with lower free methionine and S-adenosylmethionine (SAM) and higher methionine synthase (mtr) mRNA levels in both selenium-supplemented treatments. DNA methylation profiling by reduced representation bisulfite sequencing (RRBS) identified differentially methylated cytosines (DMCs) in offspring livers. These DMCs were related to 6535 differentially methylated genes in SS:NC, 6890 in SO:NC and 7428 in SO:SS, respectively. Genes with the highest methylation difference relate, among others, to the neuronal or signal transmitting and immune system which represent potential targets for future studies.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique