Optimization of Culture Conditions and Wheat Bran Class Selection in the Production of Bacillus thuringiensis-Based Biopesticides
2022
Fayad, Nancy | Abboud, Joanna | Driss, Fatma | Louka, Nicolas | Kallassy Awad, Mireille | Université Saint-Joseph de Beyrouth (USJ) | Lebanese American University (LAU) | Toulouse White Biotechnology (TWB) ; Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) ; Institut National des Sciences Appliquées (INSA)-Communauté d'universités et établissements de Toulouse (Comue de Toulouse)-Institut National des Sciences Appliquées (INSA)-Communauté d'universités et établissements de Toulouse (Comue de Toulouse)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | جامعة صفاقس - Université de Sfax - University of Sfax | This research was funded by the research council of Saint-Joseph University of Beirut CR-USJ under grant number FS65. MKA mobility (Lebanon-France) was funded by << Allocation de perfectionnement a la formation-recherche, November 2021 >> from the << Agence Universitaire de la Francophonie >>
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Bacillus thuringiensis is the leading microbial-based biopesticide, thanks to its parasporal crystal proteins or δ-endotoxins, which are toxic to insect larvae upon ingestion. Once in the insect larvae midgut, the crystal is solubilized by the alkaline pH and the δ-endotoxins activated by proteolytic cleavage. Thanks to its high efficiency as a biopesticide, several efforts have been made to enhance its growth and δ-endotoxins production, in various types of culture media. In this study, a culture medium based on wheat bran (WB), the by-product of cereal grain milling, was used to grow Bacillus thuringiensis and produce δ-endotoxins. Using the response surface methodology (RSM), the effects of three variables were evaluated: WB particles granulometry, their concentration, and their agitation in a 48-h shake-flask culture at 30 °C. Three response parameters were targeted: δ-endotoxins production, final culture pH, and dry-matter consumption. According to the RSM results, the optimum would be at 3.7 g WB/50 mL, with a granulometry above 680 μm and agitation between 170 and 270 rpm. This study is key to developing natural and cheap culture media that can be used at an industrial level for Bacillus thuringiensis-based biopesticides.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique