api , A Novel Medicago truncatula Symbiotic Mutant Impaired in Nodule Primordium Invasion
2008
Teillet, Alice | Garcia, Joseph | de Billy, Françoise | Gherardi, Michèle | Huguet, Thierry | Barker, David | de Carvalho-Niebel, Fernanda | Journet, Etienne-Pascal | Laboratoire des interactions plantes micro-organismes (LIPM) ; Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) | École nationale supérieure agronomique de Toulouse (ENSAT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Genetic approaches have proved to be extremely useful in dissecting the complex nitrogen-fixing Rhizobium-legume endosymbiotic association. Here we describe a novel Medicago truncatula mutant called api, whose primary phenotype is the blockage of rhizobial infection just prior to nodule primordium invasion, leading to the formation of large infection pockets within the cortex of noninvaded root out-growths. The mutant api originally was identified as a double symbiotic mutant associated with a new allele (nip-3) of the N1P/LATD gene, following the screening of an ethylmethane sulphonate-mutagenized population. Detailed characterization of the segregating single api mutant showed that rhizobial infection is also defective at the earlier stage of infection thread (IT) initiation in root hairs, as well as later during IT growth in the small percentage of nodules which overcome the primordium invasion block. Neither modulating ethylene biosynthesis (with L-alpha-(2-aminoetboxyvinylglycine or 1-aminocyclopropane-1-carboxylic acid) nor reducing ethylene sensitivity in a skl genetic background alters the basic api phenotype, suggesting that API function is not closely linked to ethylene metabolism or signaling. Genetic mapping places the API gene on the upper arm of the M. truncatula linkage group 4, and epistasis analyses show that API functions downstream of BIT1/ERN1,LIN and LIN and upstream of NIP/LATD and the DNF genes.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique