Pluripotent stem cell lines on the farm: naïve-state pluripotency, germline chimerism, and nuclear transfer cloning
2025
Samruan, Worawalan | Acloque, Hervé | Afanassieff, Marielle | Parnpai, Rangsun | Beaujean, Nathalie | Savatier, Pierre | Suranaree University of Technology (SUT) | Institut cellule souche et cerveau / Stem Cell and Brain Research Institute (SBRI) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Génétique Animale et Biologie Intégrative (GABI) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | This work was supported by the Agence Nationale pour la Recherche (contracts ANR-18-CE13-023, ORYCTOCELL; ANR-21-CE20-0018-01, CHROMNESS; ANR-19-CE20-0019, PluS4PiGs), the Fondation pour la Recherche Médicale (EQU202303016295 to P.S.), the Infrastructures Nationales en Biologie et Santé (ANR-11-INBS-0009, INGESTEM; ANR-11-INBS-0003, CRB-Anim), the LabExs (ANR-10-LABX-73, REVIVE; ANR-10-LABX-0061, DEVweCAN; ANR-11-LABX-0042, CORTEX), the University of Lyon within the program “Investissements d’Avenir” (ANR-11-IDEX-0007), the Suranaree University of Technology, and the Program Management Unit for Human Resources & Institutional Development, Research and Innovation (PMU-B), Government of Thailand (Grant Number: B16F640104). Worawalan SAMRUAN was supported by a PhD grant from MEDEZE Foundation (Thailand). | ANR-19-CE20-0019,PluS4PiGs,Production de véritables lignées pluripotentes porcines: une étape clé pour le phénotypage moléculaire à grande échelle des caractères agronomiques(2019) | ANR-11-INBS-0009,INGESTEM,INFRASTRUCTURE NATIONALE D'INGENIERIE DES CELLULES SOUCHES PLURIPOTENTES(2011) | ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011) | ANR-10-LABX-0061,DEVWECAN,Development Cancer and Targeted Therapies(2010) | ANR-11-LABX-0042,CORTEX,Construction, Fonction Cognitive et Réhabilitation du Cerveau(2011) | ANR-11-IDEX-0007,Avenir L.S.E.,PROJET AVENIR LYON SAINT-ETIENNE(2011)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. In this review article, we first provide an overview of research on pluripotent stem cells (PSCs) in livestock species, specifically rabbits, cattle, pigs, sheep, and goats. We discuss the two major families of PSCs: embryonic stem cell–like cells derived from pre-implantation embryos and induced PSCs generated by reprogramming somatic cells. We then critically examine the pluripotency states of these cell lines, including primed, naïve, and extended potential pluripotency. In the second section, we address the strengths and limitations of these different PSC lines across livestock species, with particular attention to their ability to capture the naïve state, form chimeric individuals, and produce cloned individuals following transfer into embryos or enucleated oocytes. Lastly, we propose several strategies to enhance cell quality for biotechnological applications, with a focus on epigenome regulation.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique