IN-VITRO ANTIBACTERIAL EFFECT OF COMBINING VITAMIN C WITH ENROFLOXACIN AGAINST QUINOLONE RESISTANT Escherichia coli (EXPEC) ISOLATED FROM BROILER CHICKEN
2022
M. Pavithra | T. Ramasamy | S. Ramesh | A. Elamaran | G. Lavanya | B. Samuel Masilamoni Ronald
Avian pathogenic E. coli (APEC) is the causative agent of colibacillosis in broiler chicken and various antibacterials used to control the infection may lead to the development of multidrug-resistant (MDR) and pan drug-resistant bacteria. Further, antibacterial resistant bacteria also emerge as a result of inappropriate use of antibacterials in chicken, reducing the efficacy of antibacterials used for medical purposes. Keeping this in view, this study was conducted to evaluate the in vitro antibacterial property of vitamin C against resistant bacteria when used along with enrofloxacin. A total of 60 liver swabs collected from the field were used in this study. E. coli were isolated using selective media and were genotypically confirmed by amplification of Adk gene. The resistant genes (qnrA, qnrB and qnrS) were detected through PCR. E. coli isolates were subjected to antimicrobial screening against enrofloxacin and vitamin C (L- ascorbic acid) both by qualitative method and quantitative method. Kirby-Bauer disc diffusion revealed enrofloxacin resistance in 83.33 percent of samples. The MIC of enrofloxacin against resistant isolates was 90.25 μg/ml, but when vitamin C was administered, the MIC was non-significantly lowered to 86.16 μg/ml in the macro broth dilution method. It was also found that vitamin C alone exhibited antibacterial activity at a concentration of ≥ 5 mg/ml. Hence, it is likely that vitamin C may improve antibacterial effects of enrofloxacin in poultry.
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