Prerequisites for the action of mixed infections of basidiomycetes closed agrobiocenoses

Goal. The study of functional features of pathogens isolated from Agaricus bisporus depending on taxonomic groups. Methods. The subject of the study is isolates of pathogenic bacteria and isolated total RNA from Agaricus bisporus, which manifested themselves as primary infections at the stage of active growth of the fungus and are typical only for mushrooms. Research methods are biotechnological. Biotechnological research methods were used in the work. To identify the isolated cultures of microorganisms, sowing was performed to split carbon sources on synthetic media with the introduction of appropriate sugars. We used biochemical methods to determine the trophic characteristics of pathogenic bacteria in order to develop biotechnologies to control their spread. Results. Isolates 1.5 and 1.6 must have a positive reaction to oxidase. Isolates 1.2 and 13.2 had a weak reaction. Sources of monosaccharides (xylose, dextrose, galactose), polysaccharides and amino acids have been identified as sources of carbon nutrition. The reaction to the breakdown of carbohydrates showed that all isolates had an oxidative type of metabolism. We have proved that chemical contamination of raw materials, substrates, casing layer and monoculture causes mutational processes in bacteria, viruses and microscopic fungi. Conclusions. Champignon double-pore in closed agrobiocenoses (mushroom growing chambers) is affected by microorganisms, forming a complex of infections that cause significant pathologies of fungi. We found that the double-pore champignon inartificial agro biocenoses (tunnels) is affected by microorganisms, forming a complex of infections that cause significant pathologies of mushroom in the course of the experiment. To obtain high-quality mycelium is necessary to introduce technologies of modern methods of diagnostic and identification of bacteria, viruses, and other pathogens of different taxonomic groups.