Bacillus lumedeiriae sp. nov., a Gram-Positive, Spore-Forming Rod Isolated from a Pharmaceutical Facility Production Environment and Added to the MALDI Biotyper® Database
2024
Luciana Veloso da Costa | Juliana Nunes Ramos | Leticia de Sousa Albuquerque | Rebeca Vitória da Silva Lage de Miranda | Talita Bernardo Valadão | João Flávio Carneiro Veras | Erica Miranda Damasio Vieira | Stephen Forsythe | Marcelo Luiz Lima Brandão | Verônica Viana Vieira
A Gram-positive, aerobic, rod-shaped and spore-forming bacterium strain designation, B190/17, was isolated from an air monitoring sample of a Brazilian immunobiological production facility in 2017. The strain was not identifiable by biochemical methodology VITEK®: 2 or by MALDI-TOF MS with VITEK®: MS RUO and MALDI Biotyper®:. The 16S rRNA gene sequencing results showed 98.51% similarity with Bacillus wudalianchiensis FJAT 27215T, 98.28% with &lsquo:Bacillus aerolatus&rsquo: CX 253T, 97.96% with Bacillus badius MTCC 1458T, 97.63% with Bacillus xiapuensis FJAT 46582T and 97.21% with Bacillus thermotolerans SGZ8T. Biochemical data showed that the strain was alanine arylamidase-, Ala-Phe-Pro arylamidase-, ELLMAN (cysteine residues)-, leucine arylamidase-, phenyalanine arylamidase- and tyrosine arylamidase-positive. The genomic DNA G+C% content of B190/17 was 41.6 mol%. The phylogenetic, genomic taxonomy and biochemical tests suggested that B190/17 represents a novel species and should be classified as the type strain of a novel Bacillus species. The name Bacillus lumedeiriae sp. nov. was proposed. After characterization, B190/17 was added to the MALDI Biotyper®: database as Bacillus lumedeiriae sp. nov.
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