Genomic Characterization of Carbapenemase-Producing Klebsiella pneumoniae ST895 Isolates from Canine Origins Through Whole-Genome Sequencing Analysis
2025
Ronglei Huang | Wei Gao | Yue Sun | Yan Ye | Tingting Luo | Yitong Pan | Chengyang Zhang | Ang Zhou | Wenzhi Ren | Chongtao Du
The widespread application of carbapenems and other broad-spectrum antibiotics has significantly escalated the threat posed by highly drug-resistant Klebsiella pneumoniae to human public health. In this research, we isolated a carbapenem-resistant K. pneumoniae strain from the feces of pet dogs at a veterinary hospital in Changchun, Jilin Province, China. To gain insights into its genetic makeup and resistance mechanisms, we conducted comprehensive whole-genome sequencing and antimicrobial susceptibility testing on the isolated strain. Our findings revealed the presence of three distinct plasmids within the strain, classified as IncFIB&:IncFII, IncR, and IncX3. Notably, the blaNDM-5 gene, conferring resistance to carbapenems, was uniquely harbored on the IncX3 plasmid, which was devoid of any other resistance genes beyond blaNDM-5. In contrast, the remaining two plasmids, IncFIB&:IncFII and IncR, were found to encode an array of additional drug resistance genes, contributing to the strain&rsquo:s broad-spectrum resistance phenotype. The IncX3 plasmid, specifically, measures 45,829 bp in length and harbors the IS5D-blaNDM-5-Ble-MBL-PRAI cassette, which has been closely linked to the dissemination of blaNDM-5 genes in K. pneumoniae strains. We reported the blaNDM-5-carrying IncX3 in K. pneumoniae isolates recovered from the pet dog and revealed the molecular characterization. Emphasis should be placed on, and continuous monitoring carried out for, the dissemination of K. pneumoniae harboring the blaNDM-5 gene among humans, companion animals, and their related environments.
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