Determination of the Infection Dynamics of Escherichia coli O157:H7 by Bacteriophage ΦV10
2025
Michael F. Oats | Claudia P. Coronel-Aguilera | Bruce M. Applegate | Laszlo N. Csonka | Arun K. Bhunia | Andrew G. Gehring | George C. Paoli
&Phi:V10 is an Escherichia coli O157:H7-specific bacteriophage that has been used to develop luminescent reporter assays for the detection of this important foodborne pathogen. Previous work demonstrated the specificity of &Phi:V10 for infection of E.coli O157:H7 through interaction with the O157 antigen. In addition, modification of the lipopolysaccharide (LPS) via O-acetylation prevents &Phi:V10 infection in an E. coli O157:H7 expressing a phage-encoded O-acetylase gene. Through assays for phage binding, plaque formation, and lysogeny using non-O157:H7 and O157: non-H7 strains, as well as complementation of an O157:H&minus: strain, it is demonstrated in this study that both the somatic O157 antigen and flagellar H7 antigen are required for productive infection of E. coli O157:H7 by &Phi:V10. Together, the results indicate that the O157 antigen is required for phage binding and that the H7 antigen is necessary to complete the infection process.
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