Characterization of systemic oxidative stress in asthmatic adults compared to healthy controls and its association with the oxidative potential of particulate matter collected using personal samplers
2025
Santibáñez Margüello, Miguel | Nuñez Robainas, Adriana | Barreiro Portela, Esther | Expósito Monar, Andrea | Agüero Calvo, Juan | García Rivero, Juan Luis | Abascal Bolado, Beatriz | Amado Diago, Carlos Antonio | Ruiz Cubillán, Juan José | Fernández Sobaler, Carmen | García Unzueta, María Teresa | Cifrián Martínez, José Manuel | Fernández Olmo, Ignacio | Universidad de Cantabria
Inflammatory cell activation in asthma may lead to reactive oxygen species (ROS) overproduction with an imbalance between oxidant levels and antioxidant capacity, called oxidative stress (OS). Since particulate matter (PM) airborne exposure may also contribute to ROS generation, it is unclear whether PM contributes more to OS than inflammatory cell activation. In our ASTHMA-FENOP study, which included 44 asthma patients and 37 matched controls, we aimed to characterize OS using five serum markers: total ROS content, protein carbonyl content, oxidized low-density lipoprotein (OxLDL), 8-hydroxydeoxyguanosine, and glutathione. Volunteers wore personal samplers for 24 h, collecting fine and coarse PM fractions separately, and the oxidative potential (OP) was determined using two methods. We observed differences between asthmatic and non-asthmatic volunteers in some OS markers, such as OxLDL, with an adjusted mean difference of 50,059.8 ng/mL (p < 0.001). However, we did not find an association between higher PM-OP and increased systemic OS. This suggests that at our PM-OP exposure levels, OS generated by the inflammatory cells themselves is more relevant than that generated by airborne PM. This supports the idea that asthma is a heterogeneous disease at the molecular level, mediated by inflammatory cell activation, and that OS may have potential clinical implications.
اظهر المزيد [+] اقل [-]This work was supported by the Spanish Society of Pneumology (SEPAR No 1383/23; No 1616/24) and the Spanish Ministry of Science and Innovation (Project PID2020-114787RBI00, funded by MCIN/AEI/10.13039/501100011033 and “ERDF A way of making Europe”).
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Universidad de Cantabria