Development of highly pathogenic avian infl uenza and Newcastle disease surveillance means on the basis of molecular and genetic techniques
2014
Stegniy, B. | Gerilovych, A. | Stegniy, A.
Highly pathogenic avian infl uenza and Newcastle disease pose considerable danger to the countries with de- veloped poultry farming, especially in the circumstances of current complicated worldwide epizootic situation regarding these diseases. Aim. This study is aimed at creating a system of methods to identify RNA of infl u- enza and Newcastle disease viruses on the basis of PCR. Methods. The methods of molecular diagnostics and bioinformatics were used. Results. The system of primers and the methodology for the identifi cation of RNA of infl uenza A virus (for the M-gene), highly pathogenic avian influenza virus subtype H5N1 in HA and NA genes, Newcastle disease virus with the amplifi cation of highly variable region gene of F have been elaborated. Conclusions. The elaborated techniques proved to be effective in the study of both reference and field samples.
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