Methods of intensifying the tuberization process of potato seeds in vitro culture
2017
Balashova, H. | Lavrynenko, Yu. | Vozhehova, R. | Kotov, B.
Aim. To determine optimal factors of impacting the tuberization intensity of the plants of mid-early potato variety Nevska in vitro. Methods. Complex application of the laboratory, mathematical-statistical, calculative- comparative methods and systemic analysis. Results. The experimental data on the impact of photoperiod and acidity of the culture medium (pH) on the tuberization induction in culture in vitro were presented. The dependence of tuberization intensity on pH of the culture medium was demonstrated. Conclusions. Optimal indices of tuberization intensity and economic effi ciency were obtained during in vitro cultivation of the plants of mid-early Nevska variety at the photoperiod of 16 hours and pH of the culture medium of 5.3: tuberization intensity was 83.0 %, the mass of the average microtuber was 612.5 mg, the mass of microtubers per one plant was 514.4 mg, the number of microtubers with the mass over 350 mg – 75.4 % with the production profi tability of 100 % and microtuber cost price of UAH 8.08 (USD 0.3).
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