Comprehensive Molecular Profiling and Indexing of Endogenous Banana Streak Virus ( BSV ) Species in Musa spp. Germplasm
2025
Caldas, Camila Pestana | Amorim, Vanusia Batista de Oliveira | Chabannes, Matthieu | Iskra-Caruana, Marie‐line | Galzi, Serge | Nogueira Pestana, Kátia | Fortes Ferreira, Claudia | Amorim, Edson Perito | Universidade Estadual de Feira de Santana [Bahia]=State University of Feira de Santana (UEFS) | Brazilian Agricultural Research Corporation = Empresa Brasileira de Pesquisa Agropecuária (Embrapa) | Embrapa Fruticultura e Mandioca, Cruz das Almas, BA, Brazil. | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) | Département Systèmes Biologiques (Cirad-BIOS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Cirad-Dgdrs-Direction de l'impact et du Marketing de la Science (Cirad-Dgdrs-Dims) ; Direction Générale Déléguée à la Recherche et à la Stratégie (Cirad-Dgdrs) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Plant Health Institute of Montpellier (UMR PHIM) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) | This study was supported by Bill and Melinda Gates Foundation (OPP1093845) and Centre de Coopération Internationale en Recherche Agronomique pour le Développement. This research was funded by IITA/The Bill and Melinda Gates Foundation-Accelerated Breeding of Better Bananas: ID OPP1093845 and CIRAD for technical training of C.F.F.
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Banana streak virus (BSV) species belong to the family Caulimoviridae , genus Badnavirus and may occur in two forms: endogenous (eBSV, integrated into the banana genome) and episomal. One of the greatest concerns of having complete eBSV sequences integrated into the Musa balbisiana ‘B’ genome is that they can ‘awaken’ under stressful conditions, leading to systemic infection of the plants. Brazil retains one of the largest banana germplasm collections in the world, harbouring accessions with Musa acuminata ‘A’ and M. balbisiana ‘B’ genomes and all combinations of ploidy levels (diploids, triploids and tetraploids). Three hundred accessions from the banana germplasm collection and 38 in vitro accessions were analysed with primers specific to banana streak GF virus (eBSGFV and BSGFV), banana streak IM virus (eBSIMV and BSIMV) and banana streak OL virus (eBSOLV and BSOLV). The 162 accessions without the B genome did not harbour any integrated BSV sequences, as expected. All eight accessions with diploid BB genome had the infective eBSOLV and eBSGFV alleles but were not infected by episomal BSV. Conversely, nearly 14% of interspecific hybrids were infected by BSV, with a higher prevalence of the BSOLV species (13 plants) compared to BSGFV (six plants). Interestingly, no plant was infected by BSIMV. Most bananas developed by the banana genetic breeding programme have B genome in their constitution, so a screening method to detect and predict the disease is of utmost necessity. This is the first report of molecular characterisation of BSV species in such a large number of samples in Brazil.
اظهر المزيد [+] اقل [-]المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique