Identification of casein phosphopeptides released after simulated digestion of milk-based infant formulas
2005
Miquel, Esther | Alegría, Amparo | Barberá, Reyes | Farré, Rosaura | Recio, Isidra | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | Generalitat Valenciana | Ministerio de Ciencia y Tecnología (España) | European Commission
Adapted, follow-up, probiotic follow-up, toddler, and probiotic toddler infant formulas were subjected to an in vitro enzymatic procedure simulating physiological digestion. The formation and identification of casein phosphopeptides (CPPs) in the milk-based infant formulas were studied using reversed phase high-performance liquid chromatography coupled on line to an ion trap mass spectrometer. Most CPPs formed contained the cluster sequence SpSpSpEE, a mineral binding site. Phosphopeptide alpha(s2)-CN(1-19)4P was present in all formulas analyzed. Probiotic formulas released CPPs not detected in nonprobiotic formulas and probably formed by bifidobacteria action. These observations suggest that physiological digestion of these products promotes the formation of bioactive peptides with mineral carrier properties in the gastrointestinal tract, which resist further proteolysis.
اظهر المزيد [+] اقل [-]This study is part of project AGL 2000-1483 financially supported by the FEDER/CICYT (Spain). Thanks to Hero S.A. (Spain) for providing the samples and for financial support for this study. Thanks to Generalitat Valenciana for the financial support given to the Bionutest group. E.M. is the holder of a grant from the Ministerio de Ciencia y Tecnologia, Spain.
اظهر المزيد [+] اقل [-]Peer reviewed
اظهر المزيد [+] اقل [-]المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Instituto de Fermentaciones Industriales