Comparisons of Ramie and Corn Stover Silages: Effects on Chewing Activity, Rumen Fermentation, Microbiota and Methane Emissions in Goats

The study aimed to investigate the nutritional value of ramie (<i>Boehmeria nivea</i>) silage, and its consequences for chewing activity, rumen fermentation, and enteric methane (CH₄) emissions in goats, by comparing it with corn stover (CS) silage. An in vitro ruminal experiment was firstly performed to investigate the substrate degradation and fermentation of CS and ramie silage. The ramie silage diet was formulated by replacing 60% of CS silage with ramie silage (dry matter (DM) basis). Eight female Xiangdong Black goats (a local breed in Southern China, 1 to 1.2 years of age) with BW of 21.0 ± 1.05 kg were used for this experiment and were randomly assigned to either one of the two dietary treatments in a cross-over design. The ramie silage had higher crude protein (CP) and ash content and lower hemicellulose content, together with decreased (<i>p</i> < 0.05) nutrient degradation and methane production and increased (<i>p</i> < 0.05) acetate molar percentage and acetate to propionate ratio through in vitro ruminal fermentation. Feeding the ramie silage diet did not alter feed intake (<i>p</i> > 0.05), decreased (<i>p</i> < 0.05) nutrient digestibility, and increased (<i>p</i> < 0.05) chewing activity and rumination activity, with reductions (<i>p</i> < 0.05) in eating activity and idle activity. Although feeding the ramie silage diet caused a greater (<i>p</i> < 0.05) molar percentage of acetate and lower molar percentage of propionate, it decreased the rumen-dissolved CH₄ concentration and enteric CH₄ emissions (<i>p</i> < 0.05). Feeding the ramie silage diet did not alter (<i>p</i> > 0.05) the population of bacteria, protozoa, and fungi; it increased the 16S rRNA gene copies of <i>Ruminococcus flavefaciens</i> (<i>p</i> < 0.05). Further 16SrRNA gene amplicon analysis indicated a distinct bacterial composition between the two treatments (<i>p</i> < 0.05). Feeding the ramie silage diet led to a lower abundance of genera <i>Lawsonibacter</i>, <i>Sedimentibacter</i>, <i>Saccharofermentans</i>, <i>Sediminibacterium</i>, and <i>Bifidobacterium</i> (<i>p</i> < 0.05). Ramie can be an alternative forage resource to stimulate chewing activity and reduce CH₄ emissions in ruminants.