Relevance. Fertigation is the application of fertilizers, in which nutrients are dissolved in water for irrigation. This method is relevant when growing eggplant in the Rostov region, allowing effective use of fertilizers, since nutrients enter directly into the roots of plants, which eliminates their loss to evaporation or weathering. During fertigation, there is no need to carry out separate procedures for fertilization and irrigation, operations are combined into one, which reduces time and costs. The Rostov region is characterized by variable climatic conditions, including drought and strong winds. Fertigation ensures uniform and constant nutrition of plants, allowing them to better adapt to weather conditions, and can help increase yields by optimizing plant growth and development conditions.Material and methodology. In the conditions of the Rostov region, on ordinary heavy loamy chernozems, studies were conducted on the cultivation of eggplant on different backgrounds of the main application of mineral fertilizers in calculated doses before planting and using root fertilizing with complex fertilizers with trace elements with irrigation (fertigation), as well as leaf fertilizing with organomineral fertilizer Arksoil during the growing season.Results. It was revealed that mineral fertilizers and top dressing accelerated the growth and development of plants, and in the best case, the increase in indicators was 2 times higher compared with the control, while the height of the main stem, the number of lateral shoots and the number of ovaries on the plant, as well as the average weight of the fruit, significantly increased, which ensured an increase in plant productivity. The combined use of basic fertilizer and complex soluble fertilizers in the drip irrigation system has significantly increased plant productivity and increased yields to 77.82 t/ha. The application of high doses of fertilizers in combination with fertigation with water-soluble fertilizers did not actually affect the quality of fruits. The content of dry matter and nitrates remained at an optimal level.

Relevanse. The article presents information about actively spreading potential pests of agricultural crops in the Central region of the Russian Federation – slugs and snails – invertebrates belonging to the genus of gastropods. The harmfulness of these pests is manifested in a decrease in the size of the crop, a decrease in its quality, loss of marketability and fruit keeping. The most seriously affected in this regard are the planting of cabbage, lettuce, peas, turnips, potatoes and cucumbers. Snails and slugs can serve as a source of infectious diseases, various fungal spores, bacteria and parasitic helminths are often carried with the mucus produced by their bodies, which often pose a threat to the health of people and pets.Methods. Visual surveys were carried out to determine the number of pests in the field and in the adjacent territory in the conditions of the Moscow region (Lyubertsy city district). The studies were conducted in accordance with officially approved methods. Data on the species composition of pests are analyzed. The possibility of active harmful activity and potential threat of gastropods in a changing climate and their adaptation in the conditions of the Central region of the Russian Federation is considered.Results. According to the results of the conducted research, it was found that in vegetable gardens and private farms, grape snail and arable slug are most often found, in potato plantings – field (arable slug). Recommendations on slug and snail control in the private sector and farms are given.

Relevance. Trophic relationships along with competition and mutualism are the most basic and significant interactions in ecosystems. To develop, survive, and multiply, insects need to consume a certain amount of nutrients at a certain ratio. Food products are complex mixes of nutrients and non-nutritive substances (sometimes toxic ones): macronutrients (proteins, carbohydrates, and lipids), micronutrients (vitamins and minerals), and water. Some nutrients are essential; insects lack the ability to synthesize them in their bodies and must obtain them from their diet or through symbiosis with beneficial organisms. Identification mechanisms being well developed in the system “phytophagous insect – plant” allow insects to successfully spread, multiply, and feed on certain plant species. The complex of hydrolytic enzymes in the insect intestine is one of the main targets for plant defense responses because these enzymes determine the availability of structural compounds for phytophagous insects. For this reason, enzymes in the insect intestine play a key role in the adaptation of insects to the pest resistance of fodder plants. For instance, when proteinase inhibitors are synthesized in a fodder plant as the result of induced insect resistance the complex of enzymes in an insect intestine might change negating the effect of these inhibitors. The development of co-adaptations due to interactions among species in food chains depends on a complicated constellation of conflicting relationships between consumers and food objects. The mechanisms of this influence may be rooted in the allelochemical interactions in the system “phytophagous insect – plant recipient”. Allelopathic interactions are among the most complex interactions because they are constituted by direct and indirect effects. Plants when damaged by phytophagous insects activate defense responses, which incorporate several mechanisms, including an increase in the concentration of secondary metabolites, many of which are phenolic compounds.The aim of the work is to describe the mechanisms of relationships in the system “phytophage-plant”. Conclusion. Management of processes of intra-water divergence of insect-phytophages in agrobiocoenoses in order to prevent the emergence of races and populations of pests adapted to live on initially resistant to them plant forms is possible in compliance with the transition to a targeted selection of agricultural crops for resistance to a complex of pests.

Relevance. Innovations in modern greenhouse complexes necessitate the creation of new technological cucumber hybrids for them, an important component of which is their comprehensive assessment in production conditions.Materials and methods. In 2023, experiments were conducted in the Krymsk (Krasnodar Territory) SC “Gavrish” with 9 new parthenocarpic hybrids of cucumber F1 smooth type in 2 repetitions of 10 plants each. Hybrids were grown in a winter greenhouse under light culture conditions in winter-spring and summer-autumn rotations. The technology of growing the crop is maintained in accordance with the requirements adopted in the V light zone. The assessment of economically valuable features was carried out according to generally accepted methods.Results. The early and total yields, marketability of the grown hybrids in two crop rotations, the degree of their resistance to real powdery mildew and adaptability of cultivation in crop rotations have been established. A comparative assessment of the potential of new hybrids with the generally accepted F1 analogue of Meva in the region has been carried out.Conclusions. The new parthenocarpic smooth-fruited cucumber hybrids of GC “Gavrish” F1 Metreng, Model 4560/19, Model 166/20 can act as an import substitution.

Relevance. Nowadays the popularity of Chamaenerion angustifolium as a medicinal plant and as a tea is growing noticeably. Fireweed is an eclogically plastic species, that does not require high soil fertility and is widespread in Russia. Medical and food industries use leafs as a row material of fireweed. Series of phytochemical studies of Chamaenerion raw material have shown that it contains a wide variety of pharmacologically significant compounds, that accumulate as a result of both primary and secondary metabolism. The most important groups, formed as a result of secondary metabolism, are polyphenols, including flavonoids, phenolic acids and ellagitannins (including enothein B). Concentration of these chemical compounds equals 15% and even hither of the raw material (leaf).The aim. Identification of variability and relationships between the content of various groups of phenolic compounds in raw material of Chamaenerion angustifolium, depending on ecological conditions.Methods. At the first stage of our work was used the cartographic method. Were studied specimens from 27 points, growing in ecologically different areas of the Moscow, Kaluga and Yaroslavl regions. Phenolic compounds were determined using a spectrophotometer: flavonoids in terms of rutin, tannins and the amount of phenolic compounds, using the Folin-Ciocalteu reagent in terms of gallic acid.Results. The content of polyphenols, tannins and flavonoids in raw material of fireweed, collected in different ecological and soil aspects, was measured. Measurement results were compared, using correlation analysis. As the result of content analysis, it was found that the content of polyphenols in the leaves ranged from 9 to 11%, the content of flavonoids fell within the chain range of 2.5-3.5%, tannin content – 5.0-6.5%. A close correlation was found between the content of phenolic compounds and tannins (R=0.972). It indicates, that the shikimate pathway is the main process of biosynthesis secondary metabolites in the Chamaenerion angustifolium plant. Also it means the leading role of local ecological conditions of the studied areas.

Relevance. Rhodiola rosea L. is a perennial herbaceous plant from the Crassulaceae family. The roots and rhizomes of R. rosea contain a range of biologically active compounds. This species is included in the State Pharmacopoeia of the Russian Federation. Medicinal preparations derived from them are widely used as adaptogens, general tonic and anti-stress agents. While the roots have been extensively studied, less attention has been given to the biologically active compounds found in the leaves of R. rosea. An introduced population of R. rosea grows on the territory of the botanical garden of medicinal plants of the All-Russian scientific research Institute of medicinal and aromatic plants (VILAR). The possibility of cultivating this species in the conditions of the Moscow region is being studied.Material and Methodology. The objective of this study was to investigate the composition of phenolic compounds in R. rosea leaves from VILAR biocollection using ultra-efficient liquid chromatography combined with mass spectrometry. Extraction was carried out with 80% acetone. An ultra-high performance liquid chromatographic system with a photodiode array detector and mass spectrometer was used for the analysis of phenolic compounds. Identification was carried out using UV- and mass spectrometry data.Results. As a result of UPLC-DD-MS analysis, peaks of 20 phenolic compounds were detected in R. rosea leaves, of which 19 compounds were tentatively identified. The leaf extract contained eight phenolic compounds from the group of galloyl-glucoses (three isomers of monogalloyl-glucose, trigalloyl-glucose, two isomers of tetragalloyl-glucose, two isomers of pentagalloyl-glucose), two gallotannins (isomers of hexagalloyl-glucose), five flavonoids (four herbacetin derivatives and one gossypetin), phenolic acids (caffeoyl-malic and ferulic acids), arbutin and esculetin. It has been shown that the biologically active substances of R. rosea provide prospects for the use of leaves as raw materials for the development of herbal preparations with anti-inflammatory, antiviral and antitumor effects.

Relevance. Eating a natural product of seedlings can completely replenish the body with missing vitamins, but there are problems in obtaining seedlings – damage to seeds by a complex of phytopathogens. Obtaining high-quality soy seedlings is an urgent task.The purpose of the research is to identify the main problems associated with the manifestation and removal of pathogenic microbiota in the production of vegetable soybean seedlings.Material and Methods. The seeds of domestic and foreign soybean varieties served as the material. Patent No. 2349098 of the Russian Federation. The determination of germination and infection of seeds was carried out according to GOST 12038-84 in 4 repetitions. Methods of disinfection of seeds: ultraviolet radiation and ozonation.Results and Discussion. It was revealed that the Hidaka variety has a high seed contamination of 48.7%, and low germination. The varieties Sibiryachka and Lydia have high germination, pathogen infestation is 6 and 8%, respectively. The contamination of the seeds of the Bar variety was a minimum of 2.7%, this variety was taken into further work. Pathogens from the genera Mucor spp., Fusarium spp., Alternaria spp., Pythium spp., Penicillium have been isolated. The phases of water consumption of vegetable soybeans have been determined, which makes it possible to determine the weight of the seed in a specific period of time and use the data for pre-sowing seed treatment. Ecological methods of disinfection of seed material have been tested – treatment with ultraviolet radiation, the use of ozone washing and ozonation of water. An analysis of the percentage of the yield of healthy and affected seedlings, depending on the method of seed treatment, showed that the untreated option has the lowest number of healthy seedlings – 10.76%. UV treatment of dry seeds and treatment in an ozone sink allowed to increase the yield of healthy seedlings to 14.24 and 14.30%, respectively. UV treatment of soaked seeds allowed to increase the yield of healthy products by 62%, and the option of using an ozone sink with further use of ozonated water allowed to obtain the maximum yield of healthy seedlings – 20.04%, which is 2 times higher relative to the control.

Relevance. Tomato late blight caused by Phytophthora infestans can cause almost 100% yield loss in open ground in cool and humid conditions. At the same time, the genetic characteristics of P. infestans allow it to overcome the genetic resistance of host plants over time, which requires breeders to look for new genes for resistance to late blight and to obtain new varieties that have several resistance genes at once.Material and methods. 12 tomato lines, or a total of 335 plants, were obtained from the N.N. Timofeev breeding station collection and planted in an artificially infected background. For molecular genetic screening, 12 plants from the Kr6 line were used. The markers linked to the late blight resistance genes Ph-3, R1, and R3a were utilized.Results. On an artificial infectious background, phenotypic evaluation of tomato resistance to late blight showed 1 line of plants free of pathogen damage, 5 lines of plants fully afflicted by late blight, and 6 lines with only partial plant damage. According to molecular genetic investigation the resistant plants were heterozygotes for the Ph-3 gene. Furthermore, the R1 gene was present in most of the plants under study; but, without the Ph-3 gene, this gene did not provide plant resistance against late blight.Conclusions. The results of this research led to the selection of tomato plants for further breeding that were resistant to late blight. It was shown that markers linked to the Ph-3 and R1 genes might be used for marker-mediated selection. Furthermore, it has been established that tomato plants are more effectively protected against P. infestans when several resistance genes are present.

Relevance. Tomato occupies a special place among vegetable crops in the world, including in the Republic of Dagestan. The article presents the results of research on the use of biostimulators of growth on the productivity of tomato plants in open ground conditions of the Tersk-Sulak subprovincion of Dagestan. The purpose of the study was to evaluate the effectiveness of the use of the biological product Relict R and Humate+7 on economically valuable indicators in the production of tomatoes of domestic selection in open ground conditions. Tomatoes were grown using conventional technology in open ground conditions.   Material and methodology. The research was carried out on the basis of the Tersko-Sulak subprovince of the Federal Agricultural Research Center of the Republic of Dagestan of the Kizlyar district. The soil of the experimental site is alluvial-meadow, medium-saline, medium loamy in mechanical composition. Agricultural technology is generally accepted in the Republic of Dagestan for tomato culture. The objects of research were tomatoes of the Voskhod VNIISSOKa variety, Relict R, Humate+7.   Results. The use of humic preparations accelerated the duration of the interphase period of germination-maturation, the difference in variants ranged from 6 to 11 days. The yield of tomatoes has shown that the use of biostimulants of growth gives a significant increase. Relict P turned out to be the most effective – the increase was 4.1 t/ha. The effectiveness of Humate+7 also exceeded the control yield and amounted to 2.3 t/ha, respectively. The use of the biopreparation stimulated the accumulation of dry substances in tomato fruits from 5.9 % in the control to 6.4-6.9 % in the experimental versions, the sugar content increased by 0.2 %. The studied preparations had a positive effect on the yield and quality indicators of tomatoes. According to the research results, it is recommended to use the Relict R – liquid humic preparation on tomato to increase yields and quality indicators in the conditions of the south of the country.

Relevance. Salvia officinalis L. is an important essential oilseed crop with anti-inflammatory, antimicrobial and antioxidant properties. Providing the pharmaceutical industry with raw materials of Salvia in full is possible due to the expansion of production areas and the availability of the maximum amount of highquality seed material of new promising varieties.Methods. In 2019-2022 in the conditions of the North Caucasus branch of VILAR on a new variety of Salvia Fioletovy aromat II year of vegetation by staging small-scale experiments were conducted to study the systematic application of organomineral fertilizer – EcoFus and growth regulators of different mechanism of action – Zircon, Epin-extra, retardant Hardy to increase seed productivity and seed quality. The area of the experimental plots was 12 m2. Treatment with EcoFus was carried out at the beginning of regrowth, and with bioregulators – during the budding phase. The consumption of the working solution is 300 l/ha. Control variants of medicinal crops were treated with water.Results. Application of EcoFus in the phase of regrowth contributed to the activation of the intensity of photosynthesis and increase in the accumulation of plastic substances, which allowed plants to enter the generative phase earlier, the budding phase came 2-3 days earlier than in the control. Subsequent treatment of budding plants with growth regulators led to earlier and more friendly flowering, the time of seed ripening was reduced by 3-4 days. The highest efficiency was observed in the variants of joint application of EcoFus with Zircon or Hardy, where the number of inflorescences on plants increased, seed setting and seed productivity increased by 37-42%, seed quality also improved – weight of 1000 seeds increased by 9-10% and germination by 6-7%.