Relevance. A study of the seasonal (phenological) phenomena and natural connections between them on the background of the relevant environmental conditions is of great scientific and practical interest. Knowledge of the dynamics of the seasonal development of woody plants helps in the selection of species and their assessment from an aesthetic and sanitary points of view, as well as in the development and implementation of measures to increase the sustainability of urban green spaces. Materials and methods. The purpose of these studies is to create a database of the dynamics of seasonal development of North American woody plants based on the results of many years of phenological monitoring (2003-2017). The 20 species and forms of North American woody introducers e of the VNIISPK arboretum genetic collection were the objects. Results. It was revealed that the growing season of woody North American plants corresponds to the climatic conditions of the forest-steppe zone of central Russia. The species with the earliest beginning of the growing season (the third decade of March) were distinguished. There were Mahonia pinnata, M. aquifolia, Betula lenta and B. lutea. On the basis of phenological calendars, phenological spectrums have been constructed. These consist of three lines (the development of leaves, shoots, flowers and fruits) with the corresponding color symbols. It has been revealed that phenospectrums vary depending on the conditions of the year. As a result, the database of 20 species and forms of North American woody plants was formed in the form of phenological spectrums against the background of registration of weather conditions in the forest-steppe zone of Russia. The database includes three options for the possible dynamics of the seasonal development of introduced North American plants. The created database allows observing, comparing, evaluating and forecasting changes in the seasonal rhythm of the studied species of introduced North American plants.

Relevance. The specifics of the fresh cut flowers market in the Russian Federation is such, that 85-100% of the total volume of flowers sold is imported. The most popular flowers among Russian consumers are roses (up to 60% in the total share of fresh flowers), chrysanthemums and carnations, all of which require certain growing conditions. Recently, the market of local seasonal flowers has begun to develop, the main players in which are small farms or household plots. Material and methods. This article presents the data about market analysis of fresh cut flowers in Russia for the last five years (2015-2020). For this analysis were used the methods applied in economics. The main information database of this research includes data from official state statistics; open data from analytical centers; federal and regional regulations; data from the Federal agency for technical regulation and Metrology; reference materials of specialized data on research field; data from industry-specific portals; sites; articles and reviews written by producers of fresh cut flowers in Russia and worldwide. Results. Russia is one of the top six importers of fresh cut flowers in the world. The most popular flower among Russian consumers is a rose. There is not much suitable soil and climatic conditions in Russia for growing it on a commercial scale in the open ground. In 2018-2019 according to Rosstat data the number of flowers, grown for cutting in Russia, was estimated 263,3 billion pieces. However, now Russian flower producers are not able to meet domestic demand for fresh flowers, so there is no almost export.

Relevance. In the conditions of the dry-steppe zone of lowland Dagestan, the beneficial effect of drip irrigation in combination with dump cultivation on the yield and quality of sweet pepper fruits from Moldova proved. Results. It experimentally established that maintaining moisture in the active 0.5 m soil layer during the growing season within 80- 100% of LMC against the background of dump processing to a depth of 0.23-0.25 m led to an increase in yield and biochemical composition of sweet pepper fruits. Differences in the influence of dump and small (0.10-0.12 m) tillage on agrophysical and phytosanitary indicators of fertility of meadow medium loamy soil were revealed. The data showing the inappropriateness of using small-scale cultivation under conditions of irrigated vegetable growing, which leads to a deterioration in the agrophysical indicators of fertility: increase in soil density by 0.05-0.08 t/m3, decrease in the number of water-resistant units by 7,0%, water permeability reduction by 22.0% and water resistance coefficient by 12.9%, 2.1 times increase in planting weed and 212.6% potential soil weed. Of the three studied thresholds of soil moisture (70, 80 and 90% LMC), it was established that the best conditions for plant growth and development, optimization of their photosynthetic activity are created at a humidity threshold of 80% LMC, at which the most efficient use of irrigation water is noted. Drip irrigation with maintaining the pre-irrigation threshold for soil moisture not lower than 80% of against the background of dump processing leads to an increase in the number of fruits on 1 plant, an increase in the mass of 1 fruit, which contributed to an increase in yield to 62 t/ha.

Relevance. New types of vegetable crops, previously unknown on a large scale, appear on the market. Japanese cabbage (Brassica rapa L. subsp. nipposinica (L. H. Bailey) Hanelt) is one of such crops for the middle zone of the Russian Federation. Due to the high content of vitamin C and microelements, Japanese cabbage can be classified as a rational balanced diet, including dietary one, and it can also be used as a raw material for the medical industry. Methods. The study of a promising sample of Japanese cabbage selection at the Federal Research Vegetable Center consisted in phenological observation of the stages of plant growth and development, morphological study of economically valuable traits, and biochemical analyzes of the content of ascorbic acid, dry matter and photosynthetic pigments. Results. The results of observations made it possible to establish the timing of the onset of phenological phases in Japanese cabbage plants and showed that the active formation of leaf mass in plants occurs 20 days after planting the seedlings. A close direct relationship was established between the duration of economic shelf life and the number of leaves in a plant (R = 0.95) and the length of the leaf plate (R=0.92). In the conducted biochemical studies, it was revealed that the content of ascorbic acid in the promising sample is 37.84±0.88 mg%, and the dry matter is in the range of 11.3±0.42%. The content of antioxidants in terms of HA and AA is within 5.72±0.72 and 19.5±2.46 mg/g, respectively. The study of the morphological characteristics and biological characteristics of Japanese cabbage plants contribute to the maximum use of the potential of vegetable crops in the conditions of the Moscow region.

Relevance. One of the most important directions in the breeding and variety study of fruits, in particular domestic plum European, is the breeding of cultivars with high commercial and consumer qualities of fruits. In this regard, the assessment of some commercial and consumer qualities of fruits in plum cultivars of the RRIFCB gene pool was of great practical and scientific interest. Material and methods. As objects of research, we used 8 plum cultivars of the RRIFCB gene pool and a control cultivar of the RRIFCB breeding Bolkhovchanka. The studies were carried out on the basis of the laboratory of breeding and variety study of stone fruit crops of RRIFCB in 2015-2019. Determination of color, shape, average weight, taste, size of fruit, size and shape of the stone, density of pulp, separation and length of the stalk, color of the juice were carried out in accordance with the "Program and methodology for the variety study of stone fruit crops" and "Pomology". Results. Most of the studied plum cultivars from the gene pool of the Russian Research Institute for Fruit Crops Breeding have valuable commercial and consumer qualities of fruits, which will allow them to be used in further breeding for this trait. Cultivars Californiyskaya krupnaya, Zarechnaya rannyaya, Volgogradskaya can be recommended for further use in breeding as sources of high fruit mass. Cultivars Californiyskaya krupnaya and Bolkhovchanka can be recommended for further use in breeding as sources of high pulp content in fruits and fruit sizes. On the basis of the tasting assessment of the fresh fruits, the fruits of the cultivar Volgogradskaya stood out positively.

Deoxyribonucleic acid (DNA) is one of the main carriers of hereditary information. The structural physicochemical information of DNA ultimately determines the structure and functioning of all living organisms. In DNA, various mutational events accumulate and recombination events occur, which lead to the variability of organisms and are subject to both natural and artificial selection. The interaction "genotype-environment" inherent in all living organisms is also characteristic of DNA, which is located in the intracellular and intranuclear physicochemical environment of water molecules, sugars, metal ions, pH, nucleotides and other components. The establishment and study of the physicochemical properties of native DNA contributes to not only understanding the mechanisms of the structure of the main hereditary biomolecule, but also to clarify their functioning, as well as interaction with other molecules at the molecular level. The discovery of various forms of double helices: A, Aʹ, B, α-Bʹ, β-Bʹ, C, Cʹ, Cʹʹ, D, E and Z suggests the idea of molecular genetic diversity existing at the DNA level and the establishment of their structural and functional features can lead to an understanding of the implementation of genetic information at the general biological level. The structure of natural DNA as a whole, apparently, does not depend on the sequence and nucleotide composition. For natural molecules - satellite DNA with repeats or DNA without repeats, the presence of only A-, B- and C-forms has been confirmed. The structure of DNA depends not only on temperature, but also on the nature of the cations present. The presence of a certain amount of metal ions in the medium can lead to the transition of the B-form of DNA to the Zform. The B ↔ Z transition modifies the general structure of DNA and, therefore, may be important for the regulation of gene expression. The study of the biological role of Z-DNA, possibly in the near future, will help to understand the mechanism of gene expression, primarily of an epigenetic nature, which has not yet been fully elucidated.

Relevance. The non-chernozem zone of Russia and the more northern regions of the country are far from the most favorable zones for the cultivation of tomatoes in open ground. The most promising for the northern zones of Russia are earlyripening, cold-resistant varieties and hybrids of tomato. The most promising in terms of productivity, resistance to short-term frosts and late blight damage are standard and semi-standard forms of tomato. Stamp forms (var. validum Brezh.) are of significant interest, both for breeding, genetic studies of tomato culture, and for production.Results. In the laboratory of selection and seed production of solanaceous crops of the FSBSI FSVC (VNIISSOK) in recent years, a whole series of standard early ripe tomato varieties for open ground has been created (Chelnok, Revansh, Pervotsvet, Argo, Evgeniya, Malinka, Otradny). The involvement of the selected standard forms in the crosses made it possible to create a number of promising lines and varieties of the semi-standard form (Perst, Pervotsvet, Blagodatny, Dolgonosik, Voskhod VNIISSOK), which significantly increases the genetic diversity of the standard forms. These varieties with a solids content of up to 7.5%, with a long shelf life. In the non-chernozem zone and the more northern regions of the country, the VNIISSOK selection varieties easily tolerate a short-term decrease in day and night temperatures to 0…-1°C, and in the southern regions drought.

Relevance. Broccoli cabbage (Brassica oleracea L. convar. botrytis (L.) Alef. var. cymosa Duch.) is widely spread around the world due to its dietetic attribute, preventive and healing effect, cooking simplicity. F1 hybrids production is the main stream in modern crop breeding as they surpass varieties in evenness and yield quality. However, it takes up to 6 years of inbreeding to obtain pure parent lines capable for hybrid creation. Thus biotechnological methods, especially doubled haploids production technology shell be implemented in selection process. 100% homozygous line can be worked out in one generation by this technology. Nevertheless earlier researches revealed such plants low seed set that hinder their application in mass production. Therefore after pollination seed set level identification is an important step in applying lines gained via in vitro process.Materials and methods. Broccoli cabbage regenerated plants produced by isolated microspore in vitro culture method were used as raw material in our study. Flower and bud pollination was held in climate cells.Results. Study of 11 broccoli cabbage DH-lines disclosed genotype influence the ability to set seeds after bud pollination. 93.3% genotype A and 95.5% A1.3 pods were seedless in our experiments. Most genotypes demonstrated 50.3-85.7% seed set level. Low and middle ability to form seeds prevailed mainly. This peculiarity should be considered while dealing with regenerated plants by raising the number of crossings for successful breeding.

Relevance. Development of valuable sterile lines of sweet pepper using nuclear-cytoplasmic male sterility is associated with the search for stable sterility maintainers in collection material.Material and methods. The purpose of the research is to optimize the identification of the desired genotypes and accelerate the development of sterile lines using artificial climate chambers. The research material was a sterile line msTol55, which under the conditions of a film greenhouse showed partial fertility at the beginning or at the end of the growing season. The place of cultivation is artificial climate chamber, a film spring greenhouse.50-day-old seedlings were planted in pots on 10.01, the period of growing plants in the chamber - until 20.04, then the adult plants were transplanted into a spring film greenhouse. According to the traditional technology, the cassette seedlings of the same line were planted in the greenhouse on 15.04. The number of tested plants is 16-20 pcs. Growing mode in the chamber: 12 hours were illuminated with DRLV lamps, while the temperature was at the level of 26-28°C, from 20.00 to 8.00 the lamps were not illuminated, the temperature was 14-15°C. The actual sterility / fertility of the flowers was determined by the level of contamination of the fruits at the beginning of their formation.Results. The maintained temperature regime for growing in an artificial climate chamber made it possible to identify sterile (30%) and partially fertile plants (70%) in the first 2 weeks of flowering. When adult plants were transplanted into a spring greenhouse, the number of sterile plants increased to 56%. When growing the line msTol55 in a spring greenhouse using the usual technology, partial setting of seeded and little seeded fruits on 60% of the plants was observed from the third decade of May to the second decade of July, then all plants set only non-seeded fruits. Based on the results obtained, it is advisable to identify the breeding material for the Rf gene in the ACC, since the proposed growing regime serves as a provocative background and allows for more effective control over the manifestation of sterility.

Relevance. A large number of forms and varieties of garlic created in the selection process allowed this culture to spread almost all over the world: in temperate regions, in subtropics and even in tropical regions. Currently, the world's acreage of garlic is 1.438 million hectares, the average yield is 16.9 tons per hectare, the gross yield is 24.255 million tons. The largest producers of garlic are China, India, South Korea and Egypt. The highest yield was recorded in Uzbekistan, Egypt, China, Tadjikistan. In Uzbekistan, it is grown everywhere, mainly on household plots and small areas in farms. In the republic, varieties of garlic are South-violet (1972) and May VIR (1978). Material and methods. Since 2004, the Surkhandarya Scientific Experimental Station of the Vegetable Melon Crops and Potato Research Institute has been conducting selection work to create new varieties of garlic. As a result of the research, the Chidamli variety was introduced and, since 2016, introduced into the State Register. All zoned varieties belong to the group of autumn-sprouting varieties. The purpose of this research is to determine the optimal nutritional area for seed plants of garlic in elite seed production. The object of research was the garlic variety Chidamli. Results. During organization of elite seed рroduction, planting of clovers on pattern (40+15+15) x l0 sm, (40+10+10+10) x l0 sm was considered acceptable. Big and qualitatative bulbs and clovers were got bu this planting pattern. Yielding capacity was 24,4-26,2 t/ha, average weight of bulbs 63-66 g, clovers number 13,0 pcs., average weight of clovers 4,5-4,7 g.