خيارات البحث
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The effectiveness of the use of the genetic marker F295 to assess the resistance of cucumber samples to the green mottled mosaic virus (CGMMV)
2023
D. D. Teplyakova
Relevance. Cucumber green mottled mosaic virus (CGMMV) is a highly specialized virus (group Tobamovirus). It is a significant threat to cucurbits. On a high infectious background, cucumber yield losses, both in open and protected ground, can be close to 100%. The spread of the virus occurs in many ways. The most dangerous is the infected seed material. One of the effective methods of preventing CGMMV is the creation of genetically resistant hybrids to viral diseases. An experiment was conducted to determine the effectiveness of the use of the marker F295 for the selection of resistant samples.Results. When comparing the results of determining resistance against an artificial background and the results of PCR analysis for the same samples, we calculated the efficiency of the genetic marker F295, which was 65%. Since the level of effectiveness is average, for a more objective assessment, it is necessary to create another – the main marker, which will determine the direct presence of resistance to CGMMV. The use of the F295 marker is relevant in combination with other methods for deter[1]mining resistance, which will speed up and increase the efficiency of the breeding process.
اظهر المزيد [+] اقل [-]The current state of technological support for vegetable crops production in the Russian Federation
2023
Ya. P. Lobachevsky | A. S. Dorokhov | A. V. Sibirev
The main limiting factor in the development of the vegetable growing industry is the low technical level of vegetable cultivation. It should be noted that many works in domestic breeding, as well as in seed production of vegetable crops, are carried out manually. The decrease in the share of Russian varieties in the market of seed material of vegetable crops is largely due to the fact that the technological level and technical equipment of most originating institutions of Russian varieties are simply not comparable with the level of modern Western European breeding and seed production centers and companies that seek to increase the supply of seed material to the Russian market. One of the significant factors in increasing the efficiency of vegetable production is the use of highly efficient agricultural machinery and equipment. At the moment, there are no machines for performing the main operations of cultivating vegetable products in Russia, and the available agricultural machines for vegetable producers are on the verge of physical wear and tear and have long ago exhausted their depreciation resource. The existing foreign analogues presented on the Russian market do not fully meet the agrotechnical requirements imposed by Russian farmers on foreign-made machines. Based on the analysis of the technical support of the vegetable growing industry in the Russian Federation, the need for commodity producers in modern hightech complexes of machines for the production of vegetable crops is economically determined based on the total sown area of vegetable crops. The results of the statistical studies carried out made it possible to assess the current state of agricultural engineering for the production of the required number of machines for vegetable growing. The increase in sown (planting) areas and the expected increase in the production of the main types of crop products against the background of a decrease in the availability of agricultural machinery contributes to an increase in the load per unit of equipment and creates risks of a decrease in the level of agricultural mechanization. Currently, low volumes of domestic seed production are associated, among other things, with the low level of technical equipment of breeding and seed-growing organizations with specialized machines and equipment.
اظهر المزيد [+] اقل [-]Study of varieties and promising varieties of table watermelon in the conditions of the Volgograd Volga region
2022
I. N. Bocherova | N. B. Ryabchikova
Relevance. Selection and analysis of the source material for crossing varieties with an individual ability to transmit economically valuable traits to offspring is an important breeding task. Breeders conducted research aimed at obtaining highly productive varieties and hybrids of table watermelon of various ripeness groups, resistant to adverse environmental conditions and having high taste qualities in comparison with standards. When studying the varieties and varietals of table watermelon, samples were selected according to the following characteristics: the growing season, yield, bright color of the pulp, excellent taste, dry matter content.Materials and methods. Scientific research was carried out in 2019-2020 in the breeding nursery and breeding nursery in the fields of the Bykovskaya melon breeding experimental station located in the Bykovsky district of the Volgograd region. 2 varieties of Malachite, Timosha and 3 cultivars 697, 728, 750 having an advantage in valuable economic characteristics over the best zoned varieties in the middle–ripening group – Sinchevsky, and in the late-ripening group – Kholodok were studied.Results. Studies have shown that in the middle–ripening group, the new Timosha variety is the most productive (27.3 t/ha), and the variety also stood out with a high dry matter content (13.4 - 14.8%). In the late-maturing group, a variety of 750 (27.5 t/ha) was distinguished by yield. Indicators of dry matter, the percentage of dry matter higher in the cultivar 728 ranged from 13.8 to 14.8%, The tests proved the relevance of further study in the breeding nursery of selected cultivars isolated by a complex of economically valuable traits to create new promising varieties of watermelon. Work in this direction will contribute to an increase in the volume and quality of manufactured products, contribute to the further development of the melon industry.
اظهر المزيد [+] اقل [-]Species composition and identification of pathogens of real powdery mildew of pumpkin crops
2022
M. E. Sletova
When cultivating pumpkin crops in most agricultural regions of the world, one of the most aggressive diseases is real powdery mildew, the damage of plants in which, under optimal soil and climatic conditions for the pathogen, reaches 100%. In the open and protected ground, among the cultivated representatives of the Cucurbitaceae family, the most widespread and harmful are the species Sphaerotheca fuliginea (Schlecht ex Fr.) Poll and Erysiphe cichoracearum f. sp. cucurbitacearum (DC ex Merat). In regions where both phytopathogens are present, mixed infections may occur, making identification difficult, and, accordingly, further plant protection measures. The pathogens of this powdery mildew of pumpkin crops have different requirements for temperature and humidity. During the growing season, there may be a change in species composition when conditions change, for example, when a new season begins. Symptoms of the disease in the form of a white or yellowish powdery plaque in the form of rounded spots (mainly on the leaves) can manifest themselves at all stages of plant development, the susceptibility of which increases by the beginning of fruiting. According to biological characteristics, S. fuliginea and E. cichoracearum are obligate biotrophic pathogens, unable to exist independently without living photosynthetic tissue, except for the wintering (sexual) stage of teleomorphs in the form of globular kleistothecia. At this stage, it is not difficult to differentiate pathogens by the morphology of kleistothecium. When analyzing the plant material of melons with the naked eye, S. fuliginea and E. cichoracearum cause similar symptoms of damage, but by microscopy these organisms can be distinguished by a complex of morphological characteristics. Taxonomically significant features are: the location of the mycelium, the formation of conidia (single or chain), the presence or absence of noticeable fibrosin bodies, the features of the appressoria, the size and shape of the conidia, as well as the position and type of their sprout tubes. The works of Russian and foreign scientists have shown the possibility of determining the types and physiological races of pathogens using an indirect method: reactions to differentiator plants. To date, about 30 physiological races of the pathogen Podosphaera xanthii (Sphaerotheca fuliginea) and 2 races of E. cichoracearum are known. However, this method does not always correlate with the methods of molecular analysis. Determination of the species composition of powdery mildew pathogens is a starting point for the development of a further strategy for the cultivation of pumpkin crops and in breeding work for resistance to this disease.
اظهر المزيد [+] اقل [-]The effectiveness of the plant growth regulator Atonic Plus on potatoes in the conditions of the Moscow region
2022
S. V. Vasilieva | V. N. Zeyruk | M. K. Derevyagina | G. L. Belov | E. A. Kolesova
The results of evaluating the effectiveness of the plant growth regulator Atonic Plus on potatoes as a stimulator of development, immunomodulator and protectant of the negative effects of pesticides in the crop protection system in the agro-climatic conditions of the Central region of the Russian Federation (Moscow region) are presented.In the extreme weather conditions of the growing season of 2010, the growth regulator Atonic Plus effectively demonstrated its qualities in the form of the formation of adaptability and anti-stress resistance in potato plants, providing an increase in yield relative to control by 1.8-7.1% and the quality of tubers is not only compared to the control, but also relative to the reference version. The combined use of a growth regulator with a fungicide contributed to an increase in the yield of standard potatoes in early and mid-late potato varieties by 2-2.5 times compared with the control.
اظهر المزيد [+] اقل [-]Fusarium blight of common buckwheat in Primorsky krai
2022
N. V. Matsishina | S. A. Borovaya
Relevance. Common buckwheat is one of the most important cereal and melliferous crops being in demand both in Russia and overseas. Despite the fact that buckwheat has lower sus- ceptibility to infectious diseases in comparisons with other grain and cereal crops, research on its pathogens is a topical issue considering a high disease rate for this crop in Primorsky krai. Materials and methods. The study on pathogenic composition was conducted in selective crop rotations of FSBSI “FSC of Agricultural Biotechnology of the Far East named after A.K. Chaiki” at the territory of Ussuriysky district in Primorsky krai. Indeterminate (Izumrud, Pri 7, Bashkirskaya krasnostebel’naya) and determinate (Dikul) buckwheat varieties were used for the study. Fungi were isolated from rhizoplane using water washing technique, from soil and rhizosphere via the dilution method, from leaves and root systems by accumulation in a moist chamber with subsequent transfer of culture onto selective medium. Fungal isolates were cul- tivated on toxigenic medium (Myro) to determine their phytotoxicity. Phytopathogenic activity of living cultures was evaluated on potato sucrose agar according to the modified method of Chelkowski and Manka . All experiments were conducted in accordance with established methods.Results. Culture filtrates of F. avenaceum and F. graminearum isolates, obtained from different anatomical parts of common buckwheat, were characterized by low toxicity, and culture filtrates of F. oxysporum isolate were highly toxic. Phytotoxicity of pathogens manifested itself not only in decrease in laboratory germination ability of buckwheat seeds, but also in inhibition of buckwheat sprout development. It proved that metabolites exerted a prolonged effect on sensitive plants. F.avenaceum, F. oxysporum and F. graminearum have pronounced phy- topathogenic and aggressive properties in relation to buckwheat and test-plants in the laboratory conditions. The sum total of their studied phytotoxic properties is convincing enough to consider them potentially hazardous to buckwheat for wilt disease development.
اظهر المزيد [+] اقل [-]Source material creation for high content of dry soluble substances F<sub>1</sub> cherry tomato hybrids breeding
2022
S. F. Gavrish | T. A. Redichkina | A. I. Topinskiy
Relevance. Consumer demand development of exotic colors presence in modern tomato hybrids requires a timely response from domestic breeders for creating highly effective hybrids. With regard to cherry tomatoes, special attention of breeders is aimed at achieving excellent taste in new hybrids. One of the indicators is the "content of dry soluble substances in fruits". However, there is no information in the literature about the variation of this trait within different color groups of cherry tomatoes. The aim of our work was the creation of initial material for the selection of F1 cherry tomato hybrids with a high content of dry soluble substances.Materials and methods. All measurements were obtained by the refractometric method and presented in the oBrix scale. The study examined 46 F2 splitting cherry tomato populations, including 17 red, 12 yellow, 7 pink, 8 brown, and 2 purple. Analyzing the obtained data, the following statistical indicators were made: the frequency of occurrence of the variant; arithmetic mean; dispersion; the coefficient of variation; sample mean error. Relative error was used to test the sample results obtained.Results. The coefficients of variation of the trait «content of dry soluble substances in fruits» were calculated in each of the five color groups of cherry tomatoes (from 17.43% for brown-fruited to 25.13% for red-fruited). The boundaries of variability and average values of the content of dry soluble substances were determined both within the groups (from 7.2 oBr in pink-fruited to 8.8 oBr in yellow-fruited ones), and for each studied breeding sample. The practical result of the work was selection of the most promising breeding material within different color groups of cherry tomato, combining high values of dry matter with a complex of economically valuable traits.
اظهر المزيد [+] اقل [-]Results of carrot cultivation test under the conditions of the south of the Amur Region
2022
O. A. Kositsyna
Carrot is one of the main vegetable crops due to its high yield and long-term preservation during winter storage. In the Amur Region, the vegetable crop zoning is underdeveloped. As a result, the assortment of recognized varieties and hybrids of carrot has long been outdated and is not replenished with new items. As part of the research work of the Federal State Budgetary Educational Institution of Higher Education "BGPU" at its agrobiological station, the specialists perform testing and assessment of various vegetable crops, including carrot. The purpose of the study is to identify high-yielding varieties of garden carrots suitable for cultivating in the agricultural and climatic conditions of the south of the Amur Region. The field trial was performed in accordance with the methods developed by B.A. Dospekhov (1985) and the state variety testing of agricultural crops described in "Vegetable and Cucurbit Crops, Potato, and Fodder Root Crops" (1956). The researchers applied agricultural technology that is commonly used in the region. Fifteen carrot samples of foreign selection were the object of the study. The hybrid F1 Abaco that had been grown at the agrobiological station for a long time was set as a standard. The vegetative period of the studied varieties under the regional conditions is 92-110 days. Nanco F1 and Sylvano F1 showed high yields, producing 41.0 and 47.0 tons of marketable roots per ha, with an average root weight of 110.1 and 120.9 g, respectively. The marketability of harvested root crops was 89.0% for Nanco F1 and 94.0% for Sylvano F1. We recommend vegetable growers in the region to establish a cultivation conveyor for garden carrots using the mid-early Presto F1 hybrid for sale in an early bunch and high-yielding F1 hybrids Nanco and Sylvano for fall use.
اظهر المزيد [+] اقل [-]Hybrid of white cabbage Snezhinka F<sub>1</sub> – the prospects of cultivation for the organic production
2022
B. M. Molokov
Relevance. Presently, for the organic Cole crop cultivation are usually used early-ripe sorts and hybrids of Russian originator firms. There is a problem with middle and lateripe sorts and hybrids, which do not satisfy with the requirements of biological ripeness and weight of cabbage, presenting by customers. That is why hybrid Snezhinka F1 of VNIISSOK selection is of practical interest; in 2008 yr. it was shown that it is perspective to use it in the conveyer after early-ripe sorts in organic cultivation.Materials and methods. The research has done on hybrid Snezhinka F1 with comparison of hybrid Cuisor F1 (originator Syngenta seeds B.V.). The technology was maximal close to the technology of organic cultivation. The tests provided by triple repetition. The accounts and data processing were carried out by standard methods.Results. As a result, after researches of middle-ripe cabbage hybrid Snezhinka F1 was found that it satisfies the requirements of customers. Firstly, the cabbage does not contain any pesticides. Secondly, the formation of its biochemical composition proceeds without mineral fertilizers, by using the natural soil fertility. Thirdly, and it is very important by these biometric values, the cabbage reaches the phase of biological ripe at the same level or even above the popular hybrid Cuisor F1.
اظهر المزيد [+] اقل [-]Prospects for the use of leaves and inflorescences of fireweed (Chamaenerion angustifolium (L.) Scop.) as a medicinal plant material (review)
2022
M. S. Antonenko | E. L. Malankina
Relevance. Currently pharmaceutical industry, food industry and chemical laboratories demonstrate an increasing interest in natural plant sources, including Chamaenerion angustifolium. Modern researches reveal more and more medicinal properties of this plant. For this reason, the demand for the use of fireweed as a medicinal plant, especially its leaves and flowers, is very high. It means, that the study of biological properties, chemical composition, ecological and landscape features of fireweed is of scientific and practical interest.Methods. This article contains information from literary sources about pharmaceutical, chemical, biological, ecological and geographical studies of Chamaenerion, its results and recommendations. Obtained from other researches information was systematized by research topics and presented in a logical sequence.Results. The issues of using this plant in medicine and food industry are also considered here, along with the discription of collecting natural fireweed problems and the mail ways of cultivating this species. The modern condition of fireweed research is shown, a detailed analysis of fireweed biological and ecological characteristics is carried out and on this basis, the main directions for further work on the introduction of Chamaenerion into culture for its more complete and rational use in medicine and in the food industry are outlined in this article. The article presents an analysis of the problems that arise during the introduction, in particular, the improvement of vegetative reproduction techniques, the selection of populations promising in terms of the phenolic compounds content and the possibility of target compounds content in plants by the exogenous regulation.Conclusion. Despite the presence of a relatively large quantity of works devoted to Chamaenerion, some research topics are poorly covered. For example, the topic of cultivation and the use of growth regulators. The most promising directions for the future study of Chamaenerion are indicated in this article.
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