This study was carried out to investigate the possibility of utilization of barley flour in production of pan bread. wheat flour of (72% extract) was replaced by 10, 15 and 20% barley flour. The effect of such replacements on physical and chemical characteristics of produced pan bread were studied. The results indicated that barley flour had a higher contents of chemical composition than that in wheat flour except carbohydrate content was the highest in wheat flour (85.53%) than in barley flour (76.67%),whereas, protein, ash, dietary fiber, β-glucan and antioxidant were higher content in barley flour (13.63, 2.44, 19.00, 8.65, 97.34 respectively) as compared with its content in wheat flour (12.26, 0.49, 4.88, 0.34, 55.33 respctively),while, wet gluten  and falling number were reduced by added barley in composite flour. The rheological properties of the  five dough mixes were studied using farinograph and  extensograph. The water absorption and dough weakening increased as the percentage of barley flour increased, while were decreased the extensibility and maximum resistance to extension . The results of sensory characteristies of prepared bread (containes10% barley flour) was not significants different from control for crust color, grain and texture. The results revealed that it was possible to use barley flour at level of 10, 15% to produce bread that satisfied baker's and consumer's sensory expectation. This levels may be to increased the nutritional value from dietary fibers, β-glucan, minerals, vitamins and antioxidants.