The experiments were executed on the autumn and the winter of (2016 /2017, 2017/2018) seasons at Qalyub area, EL-Qalyubia governorate, Egypt. The main objective for this study to find out the efficacy of several biological and the chemical controls on growth and productivity of lettuce crop and management on white rot disease caused on Sclerotinia sclerotiorum. Thus, four treatments were applied as follow 1- the control (water application),2- Trichoderma asperellum, 3-Salicylic acid and 4- Calcium Chloride, which were evaluated and compared for the three fungicide as follow : 1- Iprodione , 2- Tebuconazole with Fluopyram and 3-Tebuconazole at the doses (85 & 100 ; 200 &100 and 50 &100 g or ml / 100L-1 respect.) . Which they tested for their ability for increasing the lettuce crop productivity and suppress mycelial growth of S. sclerotiorum pathogen by treatments were sprayed on two times on plants at (15-19 BBCH) stages by using backpack sprayer by 250 L./Fed.). Results indicated that, Iprodione applications significantly gave the highest indications of total and the Marketable yield in compared with the other experimental treatments for both seasons. Moreover, the two times of sprayes by Iprodione and Salicylic acid treatments had significantly lowered the disease incidence and disease severity and increasing the control efficiency in the both seasons. Whereas, the other treatments, Tebuconazole with Fluopyram or Tebuconazole and Trichoderma asperellum had a moderate effecacy on the lettuce crop productivety or the disease severity and incidence of Sclerotinia sclerotiorum in compared to the control treatment. In the contrast, application by Calcium Chloride had a low effect on the average yield or final yield as a mass and marketable plant in the both seasons and had insignificant effect on the disease severity or incidence of S. sclerotiorum despite low to medium recovery following applications. Finally, the control treatment was the lowest.

Seventeen bacterial isolates have been successfully isolated and purified from soil rhizosphere samples collected from different agricultural areas Buhayra, Sharqia and Alqilyubia of Egypt, and screened for production of chitinase enzymes had the highest chitinolytic activities amongst those investigated. Isolate S3-C and S1-C were Identified by biochemical, physiological tests and 16s rRNA gene primer as Bacillus cereus S3C, Gene Bank NCBI accession MK185696 and Bacillus cereus S1C accession MK185697. The production of chitinase by B.cereus S3C and Bacillus cereus S1C was optimized using colloidal chitin medium amended with 1% colloidal chitin at 30°C after five days of incubation. B.cereus S3C had potential for cell wall lysis of many phytopathogenic fungi tested such as Fusarium oxysporum and Rhizoctonia solani Potato phytopathogenic fungi by in vitro antagonistic test. The addition of B. cereus S3C chitinase was more effective than that of B.cereus S1C in increasing the resistance of Potato plants infected with various Soil- borne phytopathogenic fungi.

Possibility of manipulating some of the efficient strains of fluorescent pseudomonads to manage the root-infecting pathogens of maize was studied throughout this study.  Out of 110 isolates 24 of Pseudomonas species, recovered from the plant rhizosphere showed to have inhibitory effect against two major root-infecting pathogens of maize, namely Cephalosporium maydis and Fusarium verticillioides in vitro. Pot experiment revealed that just 4 isolates could reduce infection with both pathogens and enhance the plant growth as well. Based on the genotypic identifications of these four isolates showed that they were: Pseudomonas putida strain Pau9, P. putida strainPau11, P. putida strain Psf3 and P. aeruginosa strain Psf9.

Ninety nine yeast isolates were isolated from surface of apple, grape, orange and tomato fruits. The isolates were tested in vivo in preliminary study for biocontrol potential against green mould of navel orange fruit. According to primary screening, twenty two isolates were selected to continue the secondary screening (phase one) using different concentrations of the washed yeast cells in water suspension to evaluate their biocontrol efficacy at 21±1°C. Among twelve yeast isolates were passed to the secondary screening (phase two) at 7±1°C, washed cells of yeast isolates CT 503, CT 507, CT 508, CT 512 and CT 550 at 1x109, 2x108 and 1x108 CFU/ml produced complete protection for 21 days to wounded navel orange fruits inoculated with spore suspension of Penicillium degitatum  (1x104 conidia /ml).  Meantime, no lesions developed on the navel orange fruits treated with the yeast isolates CT 503, CT 507 (Debaryomyces hansenii var. hansenii strain C) and CT 512 (Endomycopsella vivi) at 6.6x107CFU/ml, while the percentage of rot reduction of the isolate CT 550 (Candida edax) was 99.81%. Culture filtrate of twenty two different yeast isolates used in secondary screening (phase one) did not prevent decay of wounded navel orange fruits but had an inhibitory effect on rot development. The relative abilities of the promising yeast isolates (CT 503, CT 507, CT 512 and CT 550) to induce disease resistance against P. digitatum on navel orange fruits were studied. Inoculation of promising yeast isolates significantly triggered induction of resistance in navel orange fruits. The lesion diameters of green mould 66 hours later after inoculation by spore suspension of P. digitatum  in a neighbouring wound that was made approximately 6 mm away from the initial woundwhich inoculated withthe isolates CT 512, CT 550, CT 507 and CT 503 were reduced by 25.5%, 20.5%, 16.7% and 14.1%, respectively. In this respect, there were no significant differences among the three different isolates CT 503, CT 507 and CT 550.

Different applications [various potato cultivars, chemical fertilizers, bioagents and chemical in-ducers] were used for controlling brown rot dis-ease of potato. Potato cultivars namely Diamant, Desiree, Herms, Lady Joy, Lady Rossetta, Nicola, Spunta and Valor were variably susceptible to Ralstonia solanacearum, the causal pathogen of brown rot disease. Herms cultivar was the least susceptible. Application of ammonium sulfate, potassium sulfate, Urea, and calcium superphos-phate to infested soil as a single amendment re-duced disease incidence. Application of calcium superphosphate was the most effective treatment. The bioagents Pseudomonas fluorescens, Pseu-domonas putida and Bacillus subtilis were effec-tive in controlling disease when were used sepa-rately and Pseudomonas putida was the most effi-cient. Foliar spraying with different inducers sin-gly [salicylic acid, di-potassium hydrogen phos-phate and tri-potassium phosphate] reduced the disease incidence. Tri-potassium phosphate was the most effective against the disease. Therefore, a management programme was suggested including cultivar Herms, calcium superphosphate, Pseudo-monas putida and tri-potassium phosphate, as in-tegrated treatment. Rotation of these agents showed high effectiveness to disease control as well on the yield increase.

Preparationof either Bacillus thuringiensis or Trichoderma harzianum  were used as seed coating or soil application for management of root-knot and root rot disease complex caused by the root-knot nematode, Meloidogyne javanica and the fungus Rhizoctonia solani on soybean plants. Number of galls, root galling, egg masses and disease severity were reduced sharply on plants treated with both biocontrol agents, either as seed or soil application compared with non-treated plants. Soil application with both biocontrol agents was the best method in reducing nematode and fungus disease severity. Plant growth parameters i.e. fresh shoot and root weight, dry weight and number of pods and bacterial nodules were markedly increased with all treatments of biocontrol agent either with seed or soil treatment compared to plants grown in infested soil with either pathogens alone or combined. Results show that plant survival was more affected when R. solani was alone or combined with M. javanica. Seed coating was considered the effective method in increasing survival plants. The biochemical analysis of treated plants with both biocontrol agents were affected compared to non-treated plants grown in pathogen infested soil. The biochemical parameters i.e. chlorophyll A and B, carotein, phenols and amino acids were enhanced in bioagent treated plants compared to non-treated plants

Nanotechnology takes a significant position in controlling pests. Silicon dioxide nanoparticles (SiO2-NPs) is considered one of the best alter-natives to chemical pesticides for plant crop protection from harmful arthro-pods such as phytophagous mites, Tetranychus urticae. Therefore, this study aims to evaluate the effect of (SiO2-NPs) to control T. urticae. Under green-house conditions, the results revealed, in the larval stage, a high mortality percentage (99.05±0.83%) followed by eggs (98.74±1.22%). Besides, the nymph stage recorded high mortality (94.66±1.81%) at a high concentration of 1500 ppm. While the adult females of T. urticae recorded mortality of 91.92±1.02% after 72h. Eventually, the immature stages of T. urticae were susceptible to (SiO2-NPs). Meanwhile, at laboratory evaluation on immature stages of T. urticae which resulted from live treated females during 96h showed a sharp decrease in average numbers of eggs through 96h, at 1000 and 1500 ppm. No numbers of the larval stage were recorded at 1500 ppm. No number of nymph stage were recorded at all concentrations and periods. Moreover, activity of AChE was significantly inhibited at 1500 ppm, while activity of glutathione-S-transferase was significantly increased after 72 h. Finally, (SiO2-NPs) are effective against immature stages of T. urticae under greenhouse and laboratory conditions.

Two successive field experiments during two seasons of 2008 and 2009, were carried out to evaluate some safety treatments including: mulching, tunnels, natural yeast extract, antioxidant and two rhizobacteria Serratia marcescens and Pseudomonas fluorescens towards root-knot nematode Meloidogyne spp. on strawberry plant grown in naturally infested soil and consequently their probable effects on growth parameters, chemical components and yield of strawberry compared to untreated control. Results of this study confirmed that all used treatments, significantly reduced all related nematode parameters i.e. number of galls/root system; number of egg masses/root system as well as number of juveniles/250 g soil ﴾either after 2 or 4 months﴿ when compared to plants grown in the naturally infested soil only. The highest percentage of reduction in all related nematode parameters were noticed after 4 months. Mulching, S. marcescens, P. fluorescens and tunnels, played an important role in reducing the percentage of nematode parameters. Mulching and S. marcescens reduced the percentage of gall numbers/root system by 77%, followed by tunnels by 75% and P. fluorescens by 70%. S. marcescens reduced the percentage of egg masses/root system by 80% followed by P. fluorescens by 78% and mulching by 76%. Results indicated that the soil treated with mulching, also significantly reduced the percentage of juvenile numbers/250 g soil by 79% followed by S. marcescens by 73%. Results confirmed also that all used treatments significantly increased all related plant growth characters viz. leaves, stems, root fresh and dry weight; plant height, leaves number, root length as well as false stem length. Moreover, these treatments significantly increased the activity of some oxidative enzymes e.g. peroxidase and phenoloxidase as well as some other chemical components i.e. total soluble solids (T.S.S %) and vitamin C (V.C). Results revealed that all previous treatments significantly increased the early and total yield. Our results confirmed that, the rhizobacterium S. marcescens came in the first rank as a promoter of all related plant growth characters, followed by tunnels and mulching treatments. Results confirmed that also the antioxidants, are considered the effective one in enhancing the activities of peroxidase and phenoloxidase as well as T.S.S% and V.C followed by the natural yeast extract

The evaluate some safety treatments including: mulching, tunnels, natural yeast extract, antioxidant and two rhizobacteria Serratia marcescens and Pseudomonas fluorescens towards root-knot nematode Meloidogyne spp. on strawberry plant grown in naturally infested soil and consequently their probable effects on growth parameters, chemical components and yield of strawberry compared to untreated control. Results of this study confirmed that all used treatments, significantly reduced all related nematode parameters i.e. number of galls/root system; number of egg masses/root system as well as number of juveniles/250 g soil (either after 2 or 4 months) when compared to plants grown in the naturally infested soil only. The highest percentage of reduction in all related nematode parameters were noticed after 4 months. Mulching, S. marcescens, P. fluorescens and tunnels, played an important role in reducing the percentage of nematode parameters. Mulching and S. marcescens reduced the percentage of gall numbers/root system by 77%, followed by tunnels by 75% and P. fluorescens by 70%. S. marcescens reduced the percentage of egg masses/root system by 80% followed by P. fluorescens by 78% and mulching by 76%. Results indicated that the soil treated with mulching, also significantly reduced the percent age of juvenile numbers/250 g soil by 79% followed by S. marcescens by 73%. . Results revealed that all previous treatments significantly increased the early and total yield. Our results confirmed that, the rhizobacterium S. marcescens came in the first rank as a promoter of all related plant growth characters, followed by tunnels and mulching treatments. Results confirmed that also the antioxidants, are considered the effective one in enhancing the activities of peroxidase and phenoloxidase as well as T:S.S% and V.C followed by the natural yeast extract.

Twenty dairy cows belonged to four small holder were put under study, to investigate the efficiency of using scavenger chicken (baladi) as biological control of ticks and their borne diseases. The cows were divided to two groups first and second, they were infested with ticks, Laboratory diagnosis for babesia was carried out. All samples showed positive reaction.Cloxon 2.5 gm/kg/ body weight (BW) was used to treat the cows of the two groups with the presence of ticks, and all cases were recovered. Gamatox was used to control the ticks on the cows in the first group, while scavenger chicken (5 baladi chicken in each farm), were used to control ticks in the second two farms. After seven days no tick was observed on the cows in the second group. It also showed negative result for babesia test. On the other hand, nymphs were noticed on the cows of the first group. The research recom-mended to use scavenger chicken to terminate the life cycle of ticks and to get rid of its pathogenic diseases in small scale dairy farms.