The study investigated the influence of traditional nitrogen, phosphorus and potassium (NPK) mineral fertilizers and nano-fertilizers on yield, and its attributes, and fiber characters of two Egyptian cotton varieties Giza 94 and Giza 96. The design of the experiment was a split- plot with four replicates. Results indicated that Giza 94 cultivar surpassed Giza 96 in agronomic characteristics, although Giza 96 exhibited the highest fiber reading. Nano-chitosan (NPK)-fertilization significantly improved the mean value of boll weight, seed yield, lint yield, lint percentage, upper half mean (UHM), fiber uniformity index, fiber tenacity, and Micronaire value in both seasons. Conversely, the most minimal values for the aforementioned characteristics were recorded with the control NPK fertilizer over the two seasons.