An experiment was carried out to study the effect of Bio and organic fertilizer on cucumber growth and yield during two successive seasons of 2010 and 2011 at Northern region in united Arab Emirates. The Queen cucumber hybrid was fertilized with different organic fertilizers i.e. Horse manure, compost, chickens manure and cows manure. Also using the bio fertilizer i.e. phosphobactein, Azotobacter and mycorrhizal. The results showed that cucumber yields differed in their response to the bio and organic fertilizer. The results showed that the compost was higher nitrogen percent and lower in C/N ratio, and pH, than the others organic fertilizer especially horse manure. The study demonstrated that the average cumulative cucumber yield was higher with compost + 22 (gm) mycorhizol/ plots treatments compared to other treatments throughout the experiment during the two successive winter seasons of 2010 and 2011. The plant height and plant fresh weight (g), with N100% mineral (control) deceased by 28% and 40% compared to N 100% organic (Compost) respectively during the winter season of 2010. The nitrogen, phosphorus and potassium content of cucumber fruits (%) significantly increased, as did the soil with the increase of organic fertilizes applied. The experimental results confirmed the combination of bio-and organic fertlizers could increase plant growth, yield and quality. It also confirmed that composted organic fertlizers can be used a source of nutration instead of chemical fertlizers for cucumber plants.