Paddy rice was sampled from El-Sharkia, El-Gharbia, El-Dakahlia and Kafr El-Sheikh governorates, Egypt. Of the 29 samples taken, ten were contaminated with the mycotoxin citrinin. An average of 6.79 x 104 fungal spores per gram rice was found. The isolated fungi represented 47 species belong to 28 genera. The predomi-nant genera were Aspergillus, Cladosporium and Penicillium. Aspergilli were represented by 22 species; Aspergillus niger and A. flavus had the highest occur-rence. Penicillium viridicatum produced the highest amount of citrinin on glucose ammonium nitrate salts broth and rice grains and hence, this isolate was selected as a good producer of citrinin in this study. The presence of Trichoderma hamatum re-duced the amount of citrinin produced by P. viridicatum compared with its respec-tive control. The excessive growth of T. hamatum on P. viridicatum was increased with time. Viability of P. viridicatum conidia decreased by T. hamatum with an in-crease in the incubation period. Chitinases and 1,3-B-glucanase enzyme activity of T. hamatum increased with extending the incubation period on P. viridicatum cell walls up to maximum values at 72 and 84 h, respectively. T. hamatum led to a de-crease in the production of citrinin by P. viridicatum on rice grains compared with the respective control values.