The aim of the present work to study the effect of foodtypes (sugar syrup fortified with Garlic (Allium sativum), Lemon (Citrus limon), Garlic plus Lemon), pollen grains and plain sugar syrup (1:1)) on contamination of honey with bacteria, fungi and yeasts. The data indicated that application of sugar syrup plus extracts of garlic, lemon, garlic plus lemon or bee pollen led to decrease population of bacteria, fungi and yeasts compared with control treatment (plain sugar syrup 1:1). The fungi were the least population in all the treatments compared with bacteria and yeasts, meanwhile population of bacteria were moderately and the yeasts were the most occurrence. Garlic plus lemon treatment was the most effective against population of microorganisms, but garlic and lemon separate were moderately effective and bee pollen treatment was the least effective compared with other treatments. According to isolation and identification procedures, three bacterial species (Bacillus brevis, Bacillus cereus and Clostridium botulism), four fungal species (Aspergillus apis, Aspergillus niger, Cladosporium sp. and Penicillium sp.) and three yeasts species (Debaromyces sp., Lipomyces sp. and Saccharomyces sp.) were determined according to cultural, morphological and physiological characters. Cladosporium botulism bacterium was the most frequency compared with other bacteria species, but Aspergillus apis fungus was the most frequency compared with other fungi species and Lipomyces sp. was the most frequency compared with other yeasts.

Examination was done at preliminary bracketing bioassay on one old 4th nymphal instar of desert locust. Results showed that two isolates, namely NDL1 and NDL2 were having highly potentiality as entomopathogenic bioagents. Thirty isolates were isolated from dead/ infected nymphs of desert locust occurred in raring cages at Department of Locust and Grasshoppers Research, Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt. Molecular identification of isolated bacteria was done using universal primers of 16s rRNA, followed by DNA sequencing. Nucleotides were blasted at (https://www.ncbi. nlm.nih.gov /genbank/) to recognize that NDL1 and NDL2 isolates were two different isolates of Bacillus cereus with a high similarity (100%).  Susceptibility of 4th nymphal instar of Schistocerca gregaria (Forskal) to the isolated B. cereus was determined using two bioassay procedures, Leaf-dip and per os. The insecticidal activity of both isolates against locust nymph in leaf dipping showed that NDL2 was more efficient than NDL1. However, the opposite trend was observed in using per os.  Both Isolates have the potential to be a successful biocidal agent to control desert locust.

two successive seasons of 2015 and 2016 on Wonderful“ Pomegranates trees grown in sandy soil under drip irrigation system in a private orchard located at Giza governorate, Egypt . Trees were sprayed two times (at fruits size 8-10 mm & Re- spraying at one month later) with  Potassium silicate at (2500 &  5000 ppm) , Boron at(5 &10%) , Kaolin (1 &2% ) , Zinc oxidase (1000 & 2000 ppm) with wrapping as a commercial treatments . Spraying started at July  in the two seasons to improve fruit cracking resistance “Wonderful“ pomegranates  and study their effects upon yield components, physical and chemical fruit properties. Results indicated that the “Wonderful“ pomegranates cultivar had the highest values of fruit weight and the minimum cracking values were obtained by spraying Boron at (5 %) and potassium silicate at (5000 ppm) in both seasons and this treatments gave the maximum values with total yield/tree, /feddan and Marketable yield/tree, /feddan .Meanwhile ,number of arils per fruit ,total soluble solids , total sugar ,total acidity , (arils)/fruit weight was not affected but when trees treated with wrapping, without wrapping and zinc oxidase at (1000 ppm) sprays gave the minimum mentioned above characteristics. However, fruit weight, arils weight, ascorbic acid, anthocyanin and tannins %were increased as influenced by foliar spraying with all treatments in both studied seasons. Therefore, boron at 5 % and Potassium silicate at 5000 ppm treatments could be recommended for improving Wonderfull pomegranate cvs performance in cracking resistance alternative treatments to fruit wrapping under similar conditions of this study.

The aim of this study was to investigate the use of corn distillers dried grains with solubles (DDGS) (27.11% CP) and Foots (20.84% CP) as corn processing by-products in monosex Nile tilapia, Oreochromis niloticus diets to replace yellow corn as an energy source and its effect on growth performance, nutrients utilization, whole body proximate composition, nutrients digestibility and economic efficiency. Three hundreds and seventy five fingerlings of Nile tilapia (10± 0.05 g) were randomly distributed into 15 circular plastic tanks (1000 L), representing 5 treatments. Isonitrogenous (25 to 25.84%) and isocaloric (4174.79 to 4364.65 Kcal GE/kg) experimental diets were prepared with substitution levels of DDGS 0% (T1), 20% (T2) and 40% (T3) and Foots 20% (T4) and 40% (T5). The experimental period lasted for 18 week. Results showed that both of DDGS and Foots have higher in all measured amino acids content than those in yellow corn except cysteine. Moreover, DDGS and Foots have higher content of linoleic, oleic, stearic and palmitic acids than those in yellow corn. Also DDGS and Foots covered the nutrient requirements of the essential fatty acid linoleic (18:2 ω 6) for Nile tilapia. Feed intake was significantly decreased as corn partially replaced by Foots more than the DDGS. Apparent digestibility coefficient of CP, EE, NFE and energy recorded the highest values for the T4 (75.76, 89.86%, 57.48 and 68.71%), respectively, followed by T3 with significant differences. The increasing in substitution levels of yellow corn by DDGS did not significantly (P˃0.05) had an effect on Nile tilapia performances. However weight gain and specific growth rate SGR were gradually decreased with increasing the substitution levels of Foots. The CP content of Nile tilapia carcass was significantly increased with increasing the substitution levels of DDGS (T3) and Foots (T5). Feed conversion ratio (FCR) of the fish fed diets T4 (20% Foots) was the best followed by T5 (40% Foots). The cheaper feed cost gain was obtained from T5(40% Foots) followed by T4(20% Foots).

The study aimed at estimating feed efficiency of milk production systems in Urban and Peri-urban areas in three main provinces in Egypt. The targeted provinces were: Cairo, Giza and Qaloubeya. A questionnaire has been developed to cover the variables related to the production systems. The total number of interviewers was 72, included the districts: Manshiat Elbakary; Saft Elaban and Shalaquan as peri-urban, and Elmarg; Elomrania and Dar Elsalam as urban areas. As a result of the great significant in the selected samples, a typology has processed gathering similarities in two main groups of producers: land-access and landless producers. Three crop-livestock systems have been classified due to the concepts: land capacity and herd size (group 1, 2 and 3). Dairy systems have been identified as two main urban dairy specialized systems (group 4 and 5) oriented to dairy activities. In order to measure feed efficiency for each system were represented in the groups, the most significant obtained results were: the small and the micro crop-livestock producers related land-access producers (group 2 and 3) which have small herd appear to be more efficient in valorizing feed in relation to milk production per head per year, and as well as milk production per feddan allocated to fodder crops. On contrary, the landless producers (group 4 and 5) have the lowest feed efficiency due to the amount of concentrates included in their feed strategy (intensive feeding system) and inability to produce fodders.             The large mixed crop-livestock system (group 1) represents an intermediate status that producers cultivate fodders inside farm, but remain low economic efficiency in terms of their intensive feed strategy. The results of the study indicated that the small and the micro crop-livestock (group 2 and 3) achieved the highest feed efficiency regarding milk production in Urban and Peri-urban areas and thus the importance of improving productive performance by creating supposed scenarios or technical packages using simulation systems. Nongovernmental organizations (NGOs) and extension programs are essential in performing records and marketing which help to raise product price as well as producers` income and the Gross Domestic Products (GDP).

The present study was carried out during 2015 and 2016 seasons on Kitt mongo  trees to evaluate the effect of three irrigation water management (4000  m3 of irrigation water were applied to the four physiological stages of mango tree growth as 15 & 20 & 40 & 25 % ,  30 & 15 & 35& 20 % and  35 & 20 & 30& 15 % m3 / tree/ year,  respectively) as well as  four concentrations  of potassium silicate on avoiding cold injury and  improvement yield of kitt mango .                 Irrigation Mango tree by  the second program water management (30, 15, 35 , 20) recorded The highest values of vegetative growth in terms of number of shoot /tree  and lower values of leaves percentage   affected with cold injury, Also, an evident increase in the content leaves of N  & K elements , C/N ratio  and final fruit set % total yield by divided  irrigation water at the four physiological stages of mango  trees  (30, 15, 35, 20) was recorded compared with  the other two water managements. No significant differences between the second and third irrigation water management programs (30%,  15%, 35%, 20% and  35%,  20%,  30%, 15%) on values of  leaf area, P% and  total carbohydrates as well as on TSS,  acidity and L-ascorbic acid content  of fruits .                 Spraying trees with potassium silicate in three times during the period from October to January at the rate of 1000 ppm  give the highest values of quality characteristics at mango trees and their fruits. While sprayed with potassium silicate at 2000 ppm achieve moderate values of the mentioned characteristics                 It could be recommended from the obtained data that irrigation water management at the four physiological stages (30, 15, 35, 20) of Kitt mango  trees and spraying 1000 ppm potassium silicate in dormancy period is considered  a good treatment to avoid cold damage on leaves and fruits as well as in giving the highest yield and quality of kitt fruit.

This study was carried out during three successive seasons of 2010/2011, 2011/2012 and 2012/2013 in a private orchard at 76 km Cairo, Alex. Desert road  to investigate the effect of nitrogen and potassium fertilization treatments and shading with white net on yield, and fruit quality of three-years-old mango (Mangifica indica L.) cv. Keitt grafted on sucary seedling rootstocks planted at 2.5×2.5 meters apart grown in sandy soil under drip irrigation system with wells water .This experiment included nine treatments of fertilization: T1= N 80+ K2O 80 Kg/Fed., T2= N 80+ K2O 100 Kg/Fed., T3= N 100+ K2O 80 Kg/Fed., T4= N 100+ K2O 100 Kg/Fed., T5= N 120+ K2O 80 Kg/Fed., T6= N 120+ K2O 100 Kg/Fed.,T7= N 150+ K2O 80 Kg/Fed., T8= N 150+ K2O 100 Kg/Fed. and control (T9) = N 133+ K2O 90 Kg/Fed.. The results cleared that mango Keitt cv. trees grown under white net shading condition significantly increased yield (kg)/ tree, number of fruit set/ panicle, fruit weight, TSS and ascorbic acid content. Trees supplied with 150 Kg/Fed. nitrogen + 100 Kg/Fed. potassium exhibited the highest yield (kg)/tree, number of fruit set/panicle and  fruit weight under shade . Moreover, the best fruit peel color was achieved with trees received 100 kg/feddan of nitrogen +100 kg/feddan of potassium and grown under screenhouse conditions during the three studied seasons. The higher significant value of acidity and TSS were recorded in trees supplied by 120 units of nitrogen + 100 units of potassium during first and third season. Whereas, the control treatment attained a maximum ascorbic acid content.

calorie crop and commercially used as a non-caloric sweetener for diabetic patients. It is also used as cosmetic ingredient, pickling agent, and dentifrice. Four cultivars (Spantia, Shou2A3, China, and High Sugar) of stevia were included to optimize in vitro micropropagation. Four different combinations of hormonal treatments were investigated [6-benzylamino purine (BAP) + Kinetin (Kin) (0.25 + 0.25 mg/l); Forchloefenuron (Cppu) + Kin (0.25 + 0.25 mg/l); Cppu+ Kin (0.5+0.25 mg/l); and the control medium (hormone-free)]. Out of the different media components, the hormone-free medium produced the best performance of explants. The analysis of variance showed that the control treatment was the most significant for all traits except the number of branches per cutting. Hardening of rooted plants was performed in plastic pots with 70% survival percentage during acclimatization. Molecular characterization, of the four stevia cultivars, was conducted using 11 SCoT primers. The SCoT analysis resulted in 122 amplicons, of which, 62 amplicons (51%) were polymorphic. The range of polymorphism was between 6 % and 91 %. The range of polymorphic amplicons per primer was between one and 12 amplicons. The SCoT-16 produced the highest number of polymorphic bands (12). Meanwhile, the SCoT-24 produced the least polymorphism (6 %). The current study provides a new micropropagation system with low cost, high efficiency, and hormone-free application. Additionally, the study provides the first molecular characterization of stevia using SCoT marker system. Finally, SCoT markers associated with cultivars having high and low contents of stevioside can further be validated by marker-assisted breeding studies.

Red palm weevil, Rhynchophorus ferrugineus (Oliver, 1790), Order; Coleoptera, Family; Curculionidae, is considered as the biggest threat facing date palm cultivators. The larva feed on trunk tender tissue and can cause palm death within 6-8 months after infection. Insect gut harbors a community of bacteria that lives in an endosymbiotic relationship with the insect and it is strongly evident that it plays a key role in insect life. Deciphering and identification of insect microbiota could lead to the development of new symbiotiont-based control approaches. In this work we studied the diversity of the endosymbiont bacterial community of red palm weevil larval midgut in Egypt, by using Denaturing Gradient Gel Electrophoresis (DGGE), based on 16S rRNA genes PCR amplification. We used three groups of field caught larva sampled from two different places; El-Badrashin and Agricultural Research Center (ARC) in Giza governorate, Egypt. DGGE profiling patterns has shown the relative prevalence of bacterial phylotypes  affiliated to family Firmicute; Leuconostoc lactis, Lactococcus lactis, Lactobacillus nagelli and bacterial phylotypes affiliated to family Actinobaceria; Cellulomonas cellasea, Bifidobacterium minimum, which suggest that they have a potential role in nutrition, physiology and immunomodulation of the insect.

The mycelial growth rate of three oyster mushroom strains namely Pleurotus erengii, P. ostreatus and P. florida was examined on six different agar media (malt extract, Potato dextrose, rose bengal, corn meal, czapek’s dox and waksman’s glucose agar media) to select the most suitable one. Effect of pH was also examined at different values (5.0, 5.5, 6.0, 6.5 and 7.0) on the selected medium. Results indicated that Malt extract agar medium was the most suitable one for mycelium growth of all the tested mushroom strains, being the highest (9 cm ) for P. erengii and P. ostreatus at pH 7 and 9 cm at pH 6 -6.5 for P. florida after 6 days of incubation. The mycelia growth of the tested oyster mushroom strains was also examined at different temperatures (15, 20, 25, 30 and 35ºC) and different levels of relative humidity (50, 65, 75, 85, 95 and 100 %) on malt extract agar medium. The highest mycelial growth rate was obtained at 25°C and relative humidity of 65% after 6 days of incubation for the three tested oyster mushrooms.