Plants and plant by-products are now gaining attractiveness in treatment of bacterial infections and food preservation. The objective of this study was to assess antibacterial activity of some Egyptian plant and plant by-products against the locally pathogenic isolates from patients having infectious diseases in our country. Screening of antibacterial activity of ethanol, methanol and hexane extracts of some plants: grape leaves (Vitis vinifera), mulberry leaves (Morus alba), mallow leaves (Corchorus olitorius) and lemon leaves (Citrus limon) toward Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella sp., were investigated. Antibacterial activity was performed by the agar disc diffusion method. The ethanol and methanol extract of tested plant leaves showed promising antibacterial activities against both Gram-positive and Gram-negative tested bacterial isolates due to its great ability to extract those polyphenolic and biological active compounds from natural sources which effectively act against broad spectrum bacteria. Ethanol followed by methanol were found to be the best solvents of choice to extract natural products to get maximum health and medicinal benefits. The results revealed that the extraction efficiency increase with polarity increasing of the solvents, hence the highest extraction done with ethanol and methanol and the lowest extraction with nonpolar solvent n-hexanedid not exhibit any activity against all the tested bacteria. Irradiation at 5 and 10 kGy did not significantly affect the antibacterial activity of all tested plant leaves. Results indicate the potential of these plants for further work on isolation and characterization of the active compounds responsible for antibacterial activity and its exploitation as therapeutic agents

Achieving food security is one of the main objectives of the national strategy to promote national production. The Egyptian food basket suffers from a lack of protein content. Animal production is an integral part of agricultural and national activity as a whole. The average value of Egypt's production of red meat (livestock and slaughtered animals) during the period (2000-2015) was estimated at about 24.7 billion Egyptian pounds, representing about 38.9% the value of animal production of about 63.4 billion Egyptian pounds on average, Of the value of agricultural production of about LE 179.4 billion for the same period. In the light of the results of the case study carried out in the Dina farms in 2015, it can be said that if this method is circulated using the seminal fluid at the level of the Republic 2015 for female cattle in the age group more than two years at the level of the Republic in 2015 it is expected that the number of males about 43,450 thousand head and number Females were 266,9 thousand, 13.9% and 86.1% respectively. The net realized revenues were estimated at LE 129 million for male production, while the net return on females was LE 7.87 billion, which resulted in increased female production of meat and milk, for livestock breeders in Egypt and then increase Agricultural, livestock and national agricultural income as a whole. While in the case of 2015, at the level of the Republic of the same number of cattle in the age group more than two years (at the age of the first vaccination) in the case of artificial insemination and the application of the costs and revenues of the output obtained, it is clear that the net expected return of male production in this year is about 517 million pounds While the net return on female output was LE 5.12 billion, which showed that there was a significant difference between the percentage of profits obtained. The higher the number of female herds through artificial insemination and the consequent increase in female output and the increase in meat and dairy production. Thus, the application of the method of natural vaccination of livestock in Egypt on all females expected for 2020, which is about 603.72 thousand head, the rate of milk production will be about 9100 kg per head during the production season, noting that the average milk production for the head a day about 35 kg and the number The dairy production season is about 260 days average depending on the state of the animal and the environmental and climatic conditions and the method of nutrition and care. Thus, it is expected that the total value of milk production for livestock is about 27.5 billion pounds in 2020, indicating that the increase in milk production in this way than the following method of vaccination The average value of dairy production in this case was estimated at 17.4 billion pounds for the year 2020, an increase of about 10.1 billion pounds by about 63.3%. This is in favor of the method of artificial insemination, resulting in great economic returns for individual farms, commercial farms and the national level as a whole. In the case of artificial insemination, the number of females in 2020 was estimated at 381.6 thousand. The difference is in favor of artificial insemination, with an increase of about 222.1 thousand females. In the case of artificial insemination, the number of males increases by 304,641 thousand head. And fattening of calves due to inheritance of genetic factors affecting the rate of food conversion. Since the average live weight of a live animal at slaughter is about 400 kg for females, about 450 kg for males, it is expected that the amount of meat for females in the case of artificial insemination is about 249.1 million kg in 2020 while the male meat is about 34.6 million kg. Of males in this way, and this is in favor of dairy farms. The expected amount of meat expected for females in the case of IVF is about 152.6 million kg for the year 2020, while the amount of meat from males is about 171.7 million kg. Milk production is noted in this way and is more important for meat farms So as to increase the number of males. In both cases, there is a clear difference between the profits achieved by these two techniques compared to natural vaccination, where there was a significant difference in the rate of net profit, which proves the effectiveness of artificial insemination in the achievement of self-sufficiency of meat and dairy and the promotion of the livestock sector.

Three irrigation water resources were chosen at Kafr-Saqr district, El-Sharkia Governorate to assess their qualities and their impact on soil properties. The chosen irrigation water were fresh, mixed, and drainage water applied during the two successive seasons of winter-summer of (2013 and 2014). Soil samples were collected from each location at four depths (0-20, 20-40, 40-60 and 60-100 cm). Results showed EC values of mixed and drainage water were above the normal range (which should be < 0.7 dS.m-1)). Also, the mixed and drainage irrigation waters had approximately 3.5 and 5.6 folds the salinity of the fresh water. Mean pH values of the collected irrigation water samples were within the permissible limits (6.5 to 8.4). TDS values of the fresh water were within the normal range (< 450 mg.L-1). Meanwhile, mixed and drainage water were classified as of slightly and moderately salinity hazards. The highest values of turbidity were found in the drainage water, then mixed and fresh water. SAR values of fresh water were < 1.0.  The SAR values for mixed and drainage waters ranged from 2.62 to 2.69 and from 3.68 to 3.76, respectively. BOD and COD values violated the standard limits of law 48/1982 (10 mg.L-1 for drainage water and 6 mg.L-1 for fresh water) except for the BOD values of fresh water. The increase in pH values for the soil irrigated with the mixed and drainage. The continuous irrigation of soil by wastewater resulted in continuous increase in EC values. As well as, increase the amount of total soluble salts in all layers. The application of the different irrigation waters led to increase in soluble ion.

Tumor Necrosis Factor-α (TNFα) is a cytokine signaling protein which has an important role in the immune system regulation and affect female reproductive performance. The objective of this study was investigation the TNFα gene polymorphism and its potential effects in female buffaloes fertility. The DNA was extracted from the blood of 81 buffalo females and a 592 bp fragment contains the full coding region of TNFα-exon 4 was amplified by PCR which subsequently treated with RsaI restriction enzyme. The PCR-RFLP pattern showed that all the animals had fixed CC genotype and T allele was not detected. Sequencing of amplified fragment (GenBank accession No. KY885010) flowed by sequence alignment with GenBank database revealed that the river buffalo target sequence was homologues to cattle than goat, sheep, human and mouse on DNA and amino acids levels. Comparison of TNFα amplicon with homologues Bubalus bubalis records in the GenBank detected 3 SNPs in exon 4. Two of these SNPs were synonymous while the third located in the 3` UTR. Different effects of some of discovered SNPs on RNA cis-regulatory elements and hn mRNA and mature mRNA secondary structures were predicted.

Decline of grapevine due to soil borne fungi was surveyed during 2013- 2015 summer growing seasons at El-Fayoum, El-Gharbeia and El-Beheira governorates, Egypt, Syndromes of declined grapevine plants included growth retardant of shoot system and root-rot as well as decrease of grapevine fruit yield  quality. Isolation trails from root of declined trees of different grapevine cultivars i.e., superior, flame seedless, King robi and crimson was carried out. The most soil borne fungi associated with root-rotted grapevine were Fusarium oxysporum Schlechtend, Fusarium solani (Mart.) Sacc, Botryodiplodia theobromae, Rhizoctonia solaniKuhn and Macrophomina phaseolina(Tassi) Gold. Fusarium spp. are the main fungal associated with different infection types of declined root of grapevine either a singly or in combination with B. theobromae or R. solani as second infection type and third infection type with B. theobromae + R. solani. Under soil artificially infested with 5% (w/w) of each fungal isolates inocula of fourteen isolates obtained were varied for causing wilt and root-rot symptoms of grapevine trees and reducing growth parameters plant height, root length, root size, fresh and dry weight of shoot and root than the control. Botrydiplodia theobromae isolate No. (7)was the most caused root-rot and disease severity of shoot and root of grapevine plant(100%)  followed by Fusarium avenacum caused (87.5%) of root –rot and disease severity of shoot and root. Fouthermore, Fusarium avenacum isolate was the most fungal isolate in reducing growth parameters expect root length.  This is first record that Fusarium avenacum as a causal organism causing root rot disease of grapevine in Egypt.

Chlorella vulgaris belonging to Chlorophyta and Nannochloropsis  oculata belonging to Chrythophytawere used in the currentstudy to evaluate whether their successive indoor growth using industrial food waste (okara) as a growth medium enriched with organic carbon and nitrogenThe basic nutrient solutions were BG-II for Chlorella, while F2 was used for Nannochloropsis growth. Okara was used in four concentrations ( 25 , 50 , 75 and 100% )  verses to control and based on its initial nitrogen content. Original waste was diluted by 4 fold of tape water prior inoculation. The investigated parameters were dry weigh (g.l-1); total chlorophyll (mg.l-1) and total carotenoids (mg.l-1). Maximum dry weight of Chlorella was obtained with 25% of okara waste. As for Nannochloropsis, a slight increase was observed with all okara concentrations used. Lower okara concentration (25%) enhanced chlorophyll accumulation by Chlorella vulgaris, while higher concentration (100%) reached the maximum with Nannochloropsis oculata. Completely opposite pattern was observed with total carotene.

The current study aimed to investigate the effect of microbiological treatment on improving the nutritive value of corn silage as animal feed. Dried whole corn plants were prepared and two ground silos (0.5 ton each) wereused for ensiling whole corn silage and inoculated by bacterial inoculant (containing Lactobacillus plantarum and Entrococcus faecium) at the recommended rate of 1/2g bacterial inoculants/liter of water/ton of fresh whole corn silage. The ensiling period lasted for 50 days. The criteria of response were determined by: the quality of treated corn silage, chemical characteristics and performance of rams, nutrients digestibility and nitrogen balance. Results for treated and untreated silage, respectively, showed that the silage had pH 3.71 and 3.69, Total Volatile Fatty Acids (TVFA) were 2.48 and 1.98 mmol/100ml, and NH3-N values were 6.1% and 6.41% (on dry matter basis). The values of organic matter (OM), crude protein (CP), crude fiber (CF), ether extract (EE), nitrogen free extract (NFE) and ash content on dry matter basis, however, were very close in the two rations. There were no significant differences between the two rations for the dry matter (DM). The value of CF digestibility was not significantly different from that in treated corn silage, while ration contents of treated corn silage were significantly higher in OM, CP, NFE and EE digestibility than the untreated corn silage. Recorded values for total digestible nutrients (TDN) and starch value (SV) of rations of treated corn silage were significantly higher than those of untreated corn silage being 52.64 and 63.37 vs. 43.53 and 56.09%, respectively. The digestible crude protein (DCP) values were (7.43%) for ration of treated corn silage by 5.96% for the ration of untreated corn silage.

The current study was performed to investigate effect of three water regimes as well as, three different soil conditioners on the yield and fruit quality of tomato. The studied water regimes were namely; 60, 80 and 100% of class A pan. However; the tested soil conditioners were “soil hanz”, “Olygo plus” and “Bioconditioner”. Impact of the two investigated factors was studied separately, as well as, the interaction.  The experiment was carried out at El-Dolify Farm, Khatatba, Monofia Governorate during seasons of 2012/2013 and 2013/2014. Tomato hybrid Yara F1 was used in this investigation. The obtained results confirmed that, using the “Soil hanz” due to the significant increments in all tested parameters related to the yield and the fruit quality. Moreover; interaction between “Soil hanz” and any of the three tested water regimes was significantly the best in number of fruits per plant, average fruit weight, early, marketable and total yield.  In addition; it was concluded from the results that; chemical properties of the tomato fruits were improved by applying the “Soil hanz”. Concerning the water use efficiency, it was found that, all of the tested soil conditioners resulted a significant effect related to water use efficiency compared to un-treated treatment (control).  Whereas, irrigated the tomato plants under this experimental condition confirmed the validation of using the 80% irrigation regime for irrigation without significant losses in the crop yield.

This investigation was conducted for two successive seasons (2013 and 2014) in a private vineyard located at 64 Km of Cairo-Alexandria desert road to study the possibility of using organic products to reduce powdery mildew, which it is reflect in reducing yield and fruit quality of King Ruby grapevines. The chosen vines were ten-years-old, grown in a sandy loam soil, spaced at 1.5 X 3.0 meters apart and irrigated by the drip irrigation system, trained to bilateral cordon with spur pruning, and trellised by the "Y" shape system. The vines were pruned during the last week of January with bud load of (60buds/vine). Application of different biocontrol agents Trichoderma harziamum, Trichoderma viride, Bacillus subtilis as well as blight stop a commercial biocide which contains different isolates of Trichoderma forms in Arabic gum and potassium soap) were obtained kindly from central lab. of organic agriculture, ARC. Giza, in an attempt reduces powdery mildew of the plants. The results showed that all vital bioagents treatments significantly were reduced the powdery mildew disease compared with micron sulphur and control in both season. However, spraying mixture of Trichoderma harziamum + Trichoderma viride and blight stop gave the least disease incidence and severity which it is reflect to increase yield, achieve the best physical characteristics of bunches as well as improving the physical and chemical properties of berries.

The present study aimed at identifying the degree of implementation of rural women to set technical recommendations related to health practices, determining of the relationship between the degree of implementation of the respondents to a set of recommendations for health practices and between independent variables studied, as well as to identifying the most important factors affecting the implementation of the respondents to these practices.                 To achieve the objectives of the study were selected village Hallaba and Kafr El-sabeel (famous as the village Hallaba) in Qalioub district of Qaliubiya as an area of geographic study, was chosen as a random sample howswives of the village, has reached the study sample strength (112) Researched represent 5% of the total number of housewives the families of the village, which arrived appreciation for the year 2014 (2236) family, has a questionnaire has been compiled against included many questions of like measure degree of the mothers for following of head of the household sample study of a group of technical recommendations for some rural family practices of health-related design (dependent variable), including the respect of the independent variables under study, it has lasted the test period the initial form of the questionnaire and field data collection nearly three months (April, May, June) 2015. And the current study used analytical approach through the use of a set of statistical methods in the field of data analysis, and consistent with the nature of this data, these methods have ranged from the beginning of the term, the arithmetic mean, standard deviation, simple correlation, multiple linear regression analysis. Results of statistical analysis has shown that the vast majority (83%) of heads of household were women with an executive level low and middle between the recommendations of some health practices, which refers to their need for more knowledge and change their behavior Altiv to those recommendations, and the results indicated the presence of correlation found between the degree of relationship implementation of the study sample and independent variables private sources to obtain the following health information: View health programs and health practices, the importance of health programs, TV, health unit, as variables shares (television, the importance of health programs) in the interpretation of contrast kidneys to the point of implementation of health practices by 52.4%, There is also a correlation found between the degree of implementation of the study sample the following personal, social and economic independent variables: the customs and traditions and folk legacies health practices, external openness relationship, the age of the surveyed, the economic level, as all of those four variables contributed by 72.1% in the interpretation of contrast kidneys to the point of implementation of health practices.