خيارات البحث
النتائج 1 - 10 من 71
Promoting of Abiotic Stress–Induced Resistance Using Poly-β- Hydroxybutyrate (PHB) By Rhizobium phaseoli In Common Bean Plants
2021
Shawky Selim | M. ElHaddad | Mahmoud Nassef | Wafaa Radwan | Samah Abu-Hussien
In total, 50 Rhizobium isolates were isolated from the mature root nodules of common beans plants (Phaseolus vulgaris) grown in different nine governorates of Egypt. PHB was optimized by the identified strain using response surface methodology. A total of 11 parameters (pH, incubation period, inoculum size, temperature, agitation speed, mannitol, sucrose, yeast extract, glycine, K2HPO4, and MgSO4) were analyzed for their significant effects on PHB production by the Plackett–Burman design (PBD). Sucrose, yeast extract, glycine, and MgSO4 were the main significant factors affecting PHB accumulation. Central composite design (CCD) of the response surface methodology was used to determine the optimum levels of the selected factors. Rhizobium phaseoli reached the maximum production (4.997 g/L) at run 36 in the presence of 25 g/L of sucrose, 0.0 g/L of yeast extract, 0.87 g/L of glycine, 0.3 g/L of MgSO4, and 5% of inoculation size. In vitro experiments were carried out to test the effect of different stress conditions (pH: 6–11, temperature: 5°C–50°C, salinity: 0.01%–7%, and drought: 0%–5% w/v) on the growth of Rhizobium phaseoli. The results showed that Rhizobium phaseoli can withstand 3% –5% NaCl, high temperature of 30°C– 45°C, alkalinity at pH value of 8 – 10, and drought stress at 3% – 5% w/v polyethylene glycol with growth loss of 50% when grown on modified medium and 75% when grown on the basal one. In vivo experiments were done to study the effect of drought stress levels on the growth parameters of common bean plants. In general, all the treatments with Rhizobium phaseoli grown on the modified medium were superior to Rhizobium phaseoli grown on the basal medium. Also, they showed high tolerance of drought conditions.
اظهر المزيد [+] اقل [-]Rural Women's Practices in the Field of Food Preservation and Storage in Beheira Governorate
2021
Rehab Hanafy | heba mohamed | Zeinab Abdelrahman
The research aimed to Identify the practices of rural women in preserving and storing food in a village in Beheira Governorate, Identify the level of application of the respondents to good practices in preserving and storing food, Identify the most important sources from which the respondents obtain their nutritional information on food preservation and storage, and Identify the most important factors affecting the application of Respondents practice related to food preservation and storage. The research was based on the social survey method in the sample by selecting a random sample of (164) respondents at a rate of 7% of the total (2333) Families in Al-Naqidi village in Kom Hamada district in Beheira Governorate, data were collected through a personal interview using a questionnaire form during May and June 2020, frequencies, percentages, Pearson's simple correlation coefficient and Step-Wise multiple regression analysis method were used to analyze the research data. The results indicated that about (48.8%) of the total respondents had a high level of negative practices, (43.9%) of the total respondents, their level of application is medium, while about (7.3%) of the total respondents their application of these negative practices is low the majority of respondents depend on traditional sources (television, mother and relatives and neighbors) to obtain their nutritional information for food preservation and storage. The results indicated that the most important variables responsible for the application of the research for preserving and storing food, which affected by (38.7%), are: the age of the respondent, membership of social organizations, exposure to cooking programs through various media, and contact with the agricultural guide.
اظهر المزيد [+] اقل [-]Patterns and Determinants of Egyptian Consumption of Meat and Fish
2021
Awad Basiouny | Olfat melouk | Afaf Mohamed | Tamer Adlan
The study showed that the population, imports of red meat, and retail prices of red meat are the most important factors affecting the amount of red meat consumed during the study period. But the production of white meat, the population and the retail price of white meat are the most important factors affecting the amount of white meat consumed. As for the most important factors affecting the amount of fish consumed, they were represented in fish imports, national income, retail prices of fish and retail prices of white meat. The study also found that the meat group ranks first among the food groups, with a spending rate of about 27.4% and 28.19% of the total spending on food and drink in both rural and urban areas, respectively. While fish ranked sixth with a spending rate of about 6.37% and 6.88% in both rural and urban areas, respectively for the year 2017/2018. It also showed that the annual per capita spending on both meat and fish varies according to the spending categories of the family, and the annual per capita spending on meat in urban areas has increased by 13.65%, 39.64% from its counterpart in rural in each of the second spending category (50 thousand - less than 100 thousand pounds) and the third category (100 thousand pounds or more), respectively, and an increase in the annual per capita spending on fish in urban areas by 6.1%, 23.29% and 84.17% over its counter-part in the rural in the three spending categories, respectively. The spending flexibility in urban areas compared to the countryside on the two groups of meat and fish also increased, as the spending elasticity of the meat group reached about 0.492 and 0.677 in both rural and urban areas, respectively, while the spending elasticity for fish reached about 0.369 and 0.645 in both rural and urban areas, respectively.
اظهر المزيد [+] اقل [-]Improvement of Fungal Phytase Production and Its In vitro Application in Ruminant Nutrition
2021
NOHA HASSAAN | Abd El-Nasser khattab | M. Khorshed | N. El-Bordeny | A. Abedo | M. Shoukry
Various fungal genotypes (Aspergillus niger NRRL 3135 (AN1), Aspergillus niger NRRL 326 (AN26), Aspergillus terrus F2-Kh (AT) and Mucor racemosus NRRL 3639 (MI)) were studied for their ability to produce phytase and improve the produced enzyme by ethyl-methane sulfonate (EMS) mutagenesis. AN1 showed the highest phytase activity on phytase screening medium supplemented with glucose (PSMG) after 8 days of incubation (reached 1875.40 IU/mL). The phytase activity of AN1 increased with increasing incubation time and the highest value was achieved at 12 days of incubation (2859.33 IU/mL). The exposure of AN1 spore suspension to 200 mM of EMS for different times enhanced the phytase activity and that mutant 20 Mn exhibited the highest phytase activity (reached 4520.5 IU/mL) therefore it was chosen for the next experiment. An in-vitro gas production procedure was carried out to evaluate the impact of using various amounts of laboratory produced phytase (PE) compared with commercial phytase (Axtra® PHY) on nutrients availability of ruminant's ration. Six levels (0, 400, 800, 1200, 1600 and 2000 IU phytase-1Kg dry matter) of phytase enzyme were evaluated with tested ration consisted of 40% berseem hay (BH) and 60% concentrate feed mixture (CFM). In-vitro dry and organic matter degradability (IVDMD and IVOMD), total gas production (GP), short chain fatty acids (SCFA’s) and inorganic phosphorus (Pi) concentration were improved significantly (P<0.05) by phytase addition from the two sources of phytase and the highest significant (P<0.05) values achieved at the level of 1200 IU. Results suggest that phytase activity was influenced by exposure to EMS mutagen compared to the wild type. Also, the produced enzyme source has the ability to improve the utilization efficiency of phytate diets as evidenced by the significant (P<0.05) increase in all tested parameters compared to the commercial source.
اظهر المزيد [+] اقل [-]Natural Pigments Production by Local Bacterial Isolates for Use as Antibacterial and Antioxidant
2021
Sohila El-Sayed | El-Sayed Saleh | Khadiga Aboutaleb
Among 46 local bacterial isolates capable synthetized pigments, 26% produced extracellular of blue green and fluorescent whereas 74% produced intra-cellular pigments of red, brown, yellow, orange, and rose. Seven isolates were selected as a potential candidate for the synthesis of pigments. They were Pseudomonas fluoresces, P. aeruginosa, Serratia marcescens, Azotobacter chroococcum, Micrococcus kristinae, M. luteus, and M. roseus after their morphological and biochemical studies. They capable to produce fluorescent, blue green, red, brown, orange, yellow, and rose with maximum absorbance at 400, 520, 530, 320, 440, 460, and 470 nm, with an optical density of 0.62, 1.42,1.35, 1.11, 0.45, 0.98, and 0.40, respectively. Antibacterial activity of pigments was studied against 7 bacterial pathogenic strains namely P. aeruginosa ATCC27853, Escherichia coli 0157h7 ATCC25922, Klebsiella pneumonia ATCC00607, Salmonella typhimurium ATCC25566, Listeria monocytogenes ATCC19115, Staphylococcus aureus ATCC29737, and Bacillus cereus ATCC33018. The last tested strain behaved high significant (p≤ 0.05) sensitivity for blue green, fluorescent, brown, and rose pigments while, L. monocytogenes ATCC19115 was more susceptible for red, and yellow pigments and S. aureus ATCC29737 had more inhibited by orange pigment. These bacterial pigments seemed to have antioxidant activity which inhibited the formation of diphenyl-2-picrylhydrazyl radicals with percentage ranged from 40 % to 80 %. It could therefore be stated that the bacterial pigments tested had a broad impact on foodborne pathogens and had an antioxidant effect
اظهر المزيد [+] اقل [-]Impact of Organic Fertilizers Derived from Banana and Orange Peels on Tomato plant Quality
2021
Mona Nossier
The main objective of this research is to promote the reuse of wastes resulting from different manufacturing processes such as fruit peels (e.g. banana and orange peels) as substitutes (at least partially) of mineral fertilizers. This might have positive effects in maintaining human health and reducing environmental pollution. Moreover, to compare the use of these wastes with commercial organic fertilizer i.e., compost made by Nile Company to meet the needs of tomato plants from nitrogen (N) and potassium (K) elements. To achieve the goal, two experiments were conducted. The first was an incubation experiment, which was designed to compare the organic fertilizer compost of Nile Company and the powdered banana and orange peels. The second was a field experiment achieved at the Faculty of Agriculture, Ain Shams University. Obtained results of the incubation experiment showed increases in nitrogen content by 16 to 31% and potassium by 12 to 24% due to using grinded banana and orange peels as compared to the compost treatment. Moreover, in the field experiment the concentration of nitrogen (N) and potassium (K) at different plant growth stages whether in soil or plant did not differ significantly between soil plots fertilized with mineral fertilization and those fertilized with banana and orange ones. On contrary, potassium concentration was found higher in the soil fertilized with organic fertilization than that fertilized with mineral fertilization. It has also been found to us that there are no clear significant differences between tomato fruits resulting from mineral fertilization of the ground and those that were fertilized by the mixture of banana and orange peels in terms of the ratio of nitrogen and potassium, weight, size and density of the fruit and other characteristics except for the total salts percentage, which was less Fruits fertilized with a mixture of banana and orange peels
اظهر المزيد [+] اقل [-]Effect of Green Laser Irradiation on Epididymal Camel Spermatozoa Quality Stored at 5°C
2021
Ahmed Seioudy | Esmat Abdalla | Alaa Zeidan | Farouk Khalil | Zienab Abdel-Salam | Magdy Badr | Maiada Allam
Total number of forty testes from twenty Sudani camels (Camelus dromedarius) were used in the present study (>5-10 years old and 500-600 kg body weight). The experimental work was executed to define the effect of green laser irradiation with short-wavelength 532 nm and continuous wave from a diode laser light with a total output power of 3 mW on epididymal camel spermatozoa quality at different exposure times of 0 (control, non-irradiated), 2, 4, 6, 8, and 10 min. Following irradiation, the percentages of motile spermatozoa, storagability, viability, and acrosomal damage were assessed of the epididymal camel spermatozoa stored at 5°C for 4 days. Epididymal spermatozoa was diluted with lactose-yolk-citrate (LYC) extender. The obtained results showed that the highest (P<0.05) value of the percentages of motile and storagability of spermatozoa was recorded with spermatozoa exposed to 6 min of laser irradiation and the lowest (P<0.05) value was recorded with the control group. Otherwise, the highest (P<0.05) value of the percentages of dead and acrosomal damage of spermatozoa was recorded with spermatozoa exposed to 10 min and the lowest (P<0.05) value was recorded with 2 min. The advancement of storage time at 5°C decreased (P<0.05) the percentages of motile and storagability of spermatozoa, while increased (P<0.05) the percentages of dead and acrosomal damage of spermatozoa during storage at 5°C for 4 days. Consequently, enhancing the artificial insemination program can be achieved using the laser irradiation which is considered a cost-effective technique for improving semen quality. The profitable effects of laser irradiation on epididymal camel spermatozoa quality raised the motile spermatozoa, storagability, livability, acrosomal integrity which consider an indicator to improve mitochondrial function which extends the survival of spermatozoa
اظهر المزيد [+] اقل [-]Combining Ability for Grain Yield and Some Agronomic Traits in Diallel Crosses of Bread Wheat under Nitrogen Stressed Conditions
2021
yassmin mohamed
Six bread wheat genotypes and their 15 F1 crosses were evaluated under two nitrogen fertilization levels (40 and 80 kg N/fed) to estimate combining ability for days to heading, plant height, spike length, number of spikes/plant, kernels number/spike, weight of 100-kernels and grain yield/plant during 2018/19 season. Results revealed that significant nitrogen levels (N) mean squares were detected for all studied characters. Mean squares due to genotypes (G), parents and crosses for the studied characters were significant at both nitrogen levels and their combined analyses. Also, G x N interaction was highly significant for the studied characters. The general and specific combining ability variances were found to be significant for all studied characters at both-N-levels and their combined analyses. GCA/SCA ratios were less than unity for all studied characters at both N-levels and their combined data. The behavior of gene action varied from nitrogen level to another where the interactions of GCA and SCA with nitrogen levels were significant for most studied characters. Sakha 93 was identified as the most useful source for increasing favorable alleles for grain yield/plant, kernels number/spike and weight of 100-kernels under N-stress conditions, while Giza 168 was the best combiner for earliness at the two N levels. Cross combinations; Sakha 93 x Sids 12 and Sakha 93 x Misr 1 gave the best SCA effects for grain yield/plant under N-stress conditions. Significant positive correlations were detected between means of parents and F1 crosses with their GCA and SCA effects for most studied characters under the two nitrogen levels, respectively
اظهر المزيد [+] اقل [-]The Hsp70 Expression Profiling in Fayoumi and Matrouh Chicken Subjected to Heat Stress
2021
Esraa SeifEldin | Alia Elsaudi | Khalid Fahmy | Lamiaa Radwan
The present study was undertaken to test the effect of heat stress (39 °C) for 4 hours on Hsp70 mRNA profiling in two chicken genotypes. Expression levels of hsp70 were used to assess the heat tolerance of two Egyptian local genotypes (Fayoumi and Matrouh). The expression level of the hsp70 gene is high in the Fayoumi type as opposed to the Matrouh type. In blood, after four hours of exposure, the hsp70 mRNA expression analysis revealed higher expression levels. These results showed that the acquired thermotolerance is positively linked to the stress memory. Within the two genotypes, Fayoumi type exhibited the highest means for hsp70 production, and therefore the heat stress is better tolerated, which indicates that Fayoumi mortality rate might be lower genetically under heat stress
اظهر المزيد [+] اقل [-]The Effect of Price Policies on The Most Important Economic Variables for The Potato Crop in Egypt
2021
Hussein Adam
Potato occupies a distinguished position in the Egyptian agricultural economy, whether in terms of its contribution to agricultural income or in terms of cultivated area, in addition to being one of the important food crops in the Egyptian dietary pattern in addition to being one of the most important Egyptian agricultural exports. The research aims to measure the effect of price policies on the most important economic variables of the potato crop by identifying both the productive and economic indicators of the potato crop in Egypt. The extent of deviation of the price that producers get from the real price that they must obtain. The efficiency of using agricultural resources for the potato crop. The effect of the price policy of potato crop on the product's well-being and consumer and government revenues. It was found from the results of the research that the average area cultivated with the potato crop amounted to about 317.74 thousand feddans. The feddan productivity the total production amounted to about 10.82 tons, 3477.12 thousand tons, respectively, and that the average total costs. Total income, net revenue per feddan, and farm price reached about 9554 53, 13704.05, 4149.58, and 1147.68 pounds respectively, and the ratio of total revenue to total costs. The profitability of the produced ton, and the profit of the invested pound amounted to about 1.53, 0.53 and 381.87 pounds, respectively, during the period (2000-2018), as it turned out that. The value of production inputs, the added value amounted to about 8097.89, 5606.16 pounds, and the average consumption. The amount of waste, and the surplus of the potato crop in Egypt amounted to about 2,297.89, 425.32 and 1179.22 thousand tons, respectively, and the rate of self-sufficiency of the potato crop in Egypt reached About 152.99%, the average per capita consumption of the potato crop in Egypt was about 28.51 kg / year, and the average quantity and value of exports and imports of potatoes amounted to about 418.02, 106.44 thousand tons, 145.63 and 76.47 million dollars per ton respectively, and the trade balance of potatoes reached about 7469 million Dollars during the period Previous reference to it. The nominal protection coefficient and rate and the partial equilibrium model were also estimated in order to study the effect of price policies on both the producer. The consumer, foreign trade and government revenue as a result of following a certain policy based on the indicators of welfare, efficiency and government revenue. About 0.62, 0.38, respectively, during the period (2000- 2018), as it was found that the average net economic loss. The net economic impact on society and the state, producer surplus, government revenue, and the change in foreign exchange amounted to about 247.176, 426.96, 904.352, 1309.882, 829.035 thousand pounds, respectively, during the period (2000-2018).
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