خيارات البحث
النتائج 1 - 10 من 59
Partial Gap-Filling of the Nile Tilapia Oreochromis niloticus Draft Genome النص الكامل
2023
Zoghly, Hala | Rashed, Mohamed | Magdy, Mahmoud
Partial Gap-Filling of the Nile Tilapia Oreochromis niloticus Draft Genome النص الكامل
2023
Zoghly, Hala | Rashed, Mohamed | Magdy, Mahmoud
Nile tilapia is a freshwater fish of African origin, with productive and economical characteristics affecting global fish markets. The Oreochromis niloticus reference genome has a diploid set of 22 linkage groups (LGs) in addition to ungrouped sequences. A total of 42,622 genes have been identified, but 550 genomic gaps may include more. Our study focused on some of these genomic gaps, where appropriate primers were designed and then multiplied through the polymerase chain reaction (PCR) technique. From local samples, DNA was extracted and amplified with the new primers. Successful amplicons were sequenced and annotated using available bioinformatics tools. Five assessed sequences were annotated, of which three were newly predicted in O. niloticus, as mucin 1-like, and KLR genes, and SATB region. The other two sequences belonged to grid2 and trpm7 which were previously predicted. Although individual attempts to identify genomic gaps are not feasible in filling the large lack of information on the Nile tilapia genome, a good number and valuable new information has been reached. How-ever, the following plan showed many technical problems, more time, effort, and cost that could be avoided by suggesting the application of another technique, such as whole-genome sequencing, mapping, and assembly.
اظهر المزيد [+] اقل [-]Partial Gap-Filling of the Nile Tilapia (Oreochromis niloticus) Draft Genome النص الكامل
2023
Hala Zoghly | Mohamed Rashed | Mahmoud Magdy
Nile tilapia is a freshwater fish of African origin, with productive and economic characteristics affecting global fish markets. The Oreochromis niloticus reference genome has a diploid set of 22 linkage groups (LGs) in addition to ungrouped sequences. A total of 42,622 genes have been identified, but 550 genomic gaps may include more. Our study focused on some of these genomic gaps, where appropriate primers were designed and then multiplied through the polymerase chain reaction (PCR) technique. From local samples, DNA was extracted and amplified with the new primers. Successful amplicons were sequenced and annotated using available bioinformatics tools. Five assessed sequences were annotated, of which three were newly predicted in O. niloticus, as mucin 1-like, and KLR genes, and SATB region. The other two sequences belonged to grid2 and trpm7 which were previously predicted. Although individual attempts to identify genomic gaps are not feasible in filling the large lack of information on the Nile tilapia genome, a good number and valuable new information has been reached. However, the following plan showed many technical problems, more time, effort and cost that could be avoided by suggesting the application of another technique, such as whole-genome sequencing, mapping, and assembly.
اظهر المزيد [+] اقل [-]Effect of Gamma Radiation on Ephedrine and Pseudoephedrine in Callus and Suspension Cultures of Ephedra alata النص الكامل
2023
Abdelsalam, Naglaa | Abd El-Aal, mona | Aboul Fotouh, mohamed | Reda, mohamed | Safe, Ibrahim
Effect of Gamma Radiation on Ephedrine and Pseudoephedrine in Callus and Suspension Cultures of Ephedra alata النص الكامل
2023
Abdelsalam, Naglaa | Abd El-Aal, mona | Aboul Fotouh, mohamed | Reda, mohamed | Safe, Ibrahim
Ephedra is the most widely and largest widespread genus in the Gnetopsida, a subgroup of the gymnosperms. This research was done to find out how gamma radiation affected the in vitro growth of callus and suspension cultures. In addition, the effect of gamma rays (γ-rays) on the ephedrine and pseudoephedrine concentrations was evaluated through HPLC analysis. The cell suspension and callus cultures were formed on Murashige and Skoog's basal medium (MS) supplemented with 0.5 mg/l kinetin (Kn), and 3 mg/l of 2, 4-dichlorophenoxy acetic acid (2,4-D) and exposed to variable gamma radiation doses (0, 5, 10, 15, 20, 25 and 30 Gray). Highly significant differences in both fresh and dry callus weights were recorded due to the effect of gamma rays (γ-rays). The findings demonstrated that in the cell suspension and callus cultures, 15 Gray achieved the highest fresh and dry weights when compared to the control. Similarly, the highest concentrations of ephedrine and pseudoephedrine were found in suspension and callus cultures compared to control at 15 Gray.
اظهر المزيد [+] اقل [-]Effect of Gamma Radiation on Ephedrine and Pseudoephedrine in Callus and Suspension Cultures of Ephedra alata النص الكامل
2023
Naglaa Abdelsalam | mona Abd El-Aal | mohamed Aboul Fotouh | mohamed Reda | Ibrahim Safe
Ephedra is the most widely and largest widespread genus in the Gnetopsida, a subgroup of the gymnosperms. This research was done to find out how gamma radiation affected the in vitro growth of callus and suspension cultures. In addition, the effect of gamma rays (γ-rays) on the ephedrine and pseudoephedrine concentrations was evaluated through HPLC analysis. The cell suspension and callus cultures were formed on Murashige and Skoog's basal medium (MS) supplemented with 0.5 mg/l kinetin (Kn), and 3 mg/l of 2, 4-dichlorophenoxy acetic acid (2,4-D) and exposed to variable gamma radiation doses (0, 5, 10, 15, 20, 25 and 30 Gray). Highly significant differences in both fresh and dry callus weights were recorded due to the effect of gamma rays (γ-rays). The findings demonstrated that in the cell suspension and callus cultures, 15 Gray achieved the highest fresh and dry weights when compared to the control. Similarly, the highest concentrations of ephedrine and pseudoephedrine were found in suspension and callus cultures compared to control at 15 Gray.
اظهر المزيد [+] اقل [-]Increasing of Amylopectin in Tetraploid Potato Desiree Cultivar (Solanum tuberosum L.) Via CRISPR/Cas9 النص الكامل
2023
Ali, Nermin | Abdel-Salam, Mohamed | hanfy, aiman | Abd-Elhalim, Haytham | Ahmed, Nouh | Metry, Emad
Increasing of Amylopectin in Tetraploid Potato Desiree Cultivar (Solanum tuberosum L.) Via CRISPR/Cas9 النص الكامل
2023
Ali, Nermin | Abdel-Salam, Mohamed | hanfy, aiman | Abd-Elhalim, Haytham | Ahmed, Nouh | Metry, Emad
Potato tuber starch characteristic is influenced by the ratio of amylose to amylopectin, which is affected by the granule-bound starch synthase (GBSS) gene. GBSS gene expression was decreased in the leaves of the Desiree potato variety utilizing genome editing using CRISPR-Cas9. Constructs encoding Cas9 gene and sgRNAs targeting GBSS gene were inserted into plant leaves using Agrobacterium-mediated transformation delivery. The results obtained lines with mutations in GBSS genes accounting for 21% of regenerated shoots. The identification of mutations within one base pair of the used guide sequence provided further evidence of the considerable similarity between the target region around the protospacer adjacent motif (PAM) position and the used guide sequence. Transforming DNA into potato leaves produced mutants that lacked the Cas9 gene. Using microscopic inspection of iodine-stained starch granules, the increase of amylopectin in the starch granules of editing potato tubers was evaluated.
اظهر المزيد [+] اقل [-]Increasing of Amylopectin in Tetraploid Potato Desiree Cultivar (Solanum tuberosum L.) Via CRISPR/Cas9 النص الكامل
2023
Nermin Ali | Mohamed Abdel-Salam | aiman hanfy | Haytham Abd-Elhalim | Nouh Ahmed | Emad Metry
Potato tuber starch characteristic is influenced by the ratio of amylose to amylopectin, which is affected by the granule-bound starch synthase (GBSS) gene. GBSS gene expression was decreased in the leaves of the Desiree potato variety utilizing genome editing using CRISPR-Cas9. Constructs encoding Cas9 gene and sgRNAs targeting GBSS gene were inserted into plant leaves using Agrobacterium-mediated transformation delivery. The results obtained lines with mutations in GBSS genes accounting for 21% of regenerated shoots. The identification of mutations within one base pair of the used guide sequence provided further evidence of the considerable similarity between the target region around the protospacer adjacent motif (PAM) position and the used guide sequence. Transforming DNA into potato leaves produced mutants that lacked the Cas9 gene. Using microscopic inspection of iodine-stained starch granules, the increase of amylopectin in the starch granules of editing potato tubers was evaluated.
اظهر المزيد [+] اقل [-]A Theoretical Analysis of the Plate Heat Exchanger's Ideal Operating Conditions for Producing Soybean Milk النص الكامل
2023
Salama, Omnia | Sorour, Manal | Anwer, Ashrf | Abd El-salam, Mostafa
A Theoretical Analysis of the Plate Heat Exchanger's Ideal Operating Conditions for Producing Soybean Milk النص الكامل
2023
Salama, Omnia | Sorour, Manal | Anwer, Ashrf | Abd El-salam, Mostafa
Heat exchanger optimization is a very efficient thermal design inquiry. The goal of the research is to determine an optimal channel flow velocity as well as an optimal total plate number based on overall information on the effectiveness of employing chilled water to cool soybean milk. Heat transfer coefficient, Prandtl number, Reynolds number and pressure drop measurements were applied for milk and water. The rheological properties of soybean milk have been investigated at various temperatures (5, 10, 30, 50, 70 and 90 °C). The operational circumstances of PHE in soybean procedures were investigated at different numbers of plates, flow rates and flow velocities. Based on the present study, total substances showed non-Newtonian pseudoplastic conduct in any respect at any temperature and the power law model has a decent correlation. The cost estimation of the plate heat exchanger was observed at the lowest flow velocity of (2.25) m/s and number of plates is 17.
اظهر المزيد [+] اقل [-]A Theoretical Analysis of the Plate Heat Exchanger's Ideal Operating Conditions for Producing Soybean Milk النص الكامل
2023
Omnia Salama | Manal Sorour | Ashrf Anwer | Mostafa Abd El-salam
Heat exchanger optimization is a very efficient thermal design inquiry. The goal of the research is to determine an optimal channel flow velocity as well as an optimal total plate number based on overall information on the effectiveness of employing chilled water to cool soybean milk. Heat transfer coefficient, Prandtl number, Reynolds number and pressure drop measurements were applied for milk and water. The rheological properties of soybean milk have been investigated at various temperatures (5, 10, 30, 50, 70 and 90 °C). The operational circumstances of PHE in soybean procedures were investigated at different numbers of plates, flow rates and flow velocities. Based on the present study, total substances showed non-Newtonian pseudoplastic conduct in any respect at any temperature and the power law model has a decent correlation. The cost estimation of the plate heat exchanger was observed at the lowest flow velocity of (2.25) m/s and number of plates is 17.
اظهر المزيد [+] اقل [-]Molecular Genetic Studies on Honeybees in Response to Some Environmental Stresses النص الكامل
2023
Mohamed, Rehab | Ebeed, Naglaa | Fahmy, Khalid | Alsenosy, Naima | Abdelmegeed, Sawsan
Molecular Genetic Studies on Honeybees in Response to Some Environmental Stresses النص الكامل
2023
Mohamed, Rehab | Ebeed, Naglaa | Fahmy, Khalid | Alsenosy, Naima | Abdelmegeed, Sawsan
The honeybee, Apis mellifera, is a widespread pollinator insect. Colony collapse disorder (CCD) has recently caused a significant decline in honeybee numbers worldwide. The beekeeping industry, agriculture, and world biodiversity are threatened by the honeybee population decline. We examined the mRNA levels of heat shock protein gene 60 (hsp60), acetylcholinesterase 1, and acetylcholinesterase 2, as biomolecular markers of the insect response to three separate forms of environmental stress, including high temperature, low temperature, and insecticide. The obtained results indicated increased levels of hsp60 and acetylcholinesterase 2 after the exposure of worker bees to temperatures of 17, 32, and 40°C for one hour, as well as exposure to a low dose of pesticide for 24 h ingestion of imidacloprid solution (2 ppm); meanwhile, acetylcholinesterase 1 showed remarkable expression decrease under heat and cold stresses but increased under pesticide treatment. The responses of honeybees to hsp60, acetylcholinesterase 1, and acetylcholinesterase 2 are diverse and very specific to various stimuli. According to this research, the distinct expression profiles of hsp60 and acetylcholinesterase 2 can serve as useful instruments for tracking honeybees' vulnerability to varied environmental effects. Due to its insufficient responsiveness, acetylcholinesterase 1 expression is not permitted for use as a biomarker.
اظهر المزيد [+] اقل [-]Molecular Genetic Studies on Honeybees in Response to Some Environmental Stresses النص الكامل
2023
Rehab Mohamed | Naglaa Ebeed | Khalid Fahmy | Naima Alsenosy | Sawsan Abdelmegeed
The honeybee, Apis mellifera, is a widespread pollinator insect. Colony collapse disorder (CCD) has recently caused a significant decline in honeybee numbers worldwide. The beekeeping industry, agriculture, and world biodiversity are threatened by the honeybee population decline. We examined the mRNA levels of heat shock protein gene 60 (hsp60), acetylcholinesterase 1, and acetylcholinesterase 2, as biomolecular markers of the insect response to three separate forms of environmental stress, including high temperature, low temperature, and insecticide. The obtained results indicated increased levels of hsp60 and acetylcholinesterase 2 after the exposure of worker bees to temperatures of 17, 32, and 40°C for one hour, as well as exposure to a low dose of pesticide for 24 h ingestion of imidacloprid solution (2 ppm); meanwhile, acetylcholinesterase 1 showed remarkable expression decrease under heat and cold stresses but increased under pesticide treatment. The responses of honeybees to hsp60, acetylcholinesterase 1, and acetylcholinesterase 2 are diverse and very specific to various stimuli. According to this research, the distinct expression profiles of hsp60 and acetylcholinesterase 2 can serve as useful instruments for tracking honeybees' vulnerability to varied environmental effects. Due to its insufficient responsiveness, acetylcholinesterase 1 expression is not permitted for use as a biomarker.
اظهر المزيد [+] اقل [-]Efficacy of Potassium Fertilizer and Certain Pesticides for the Management of Brinjal Fruit Infection by Shoot Borer [Leucinodes Orbonalis (Guenée)] النص الكامل
2023
bhandari, Sagar | kandel, Bikash | gyawali, Pramod | pokhrel, Saurav | ojha, Sushil | bhandari, Sarita
Efficacy of Potassium Fertilizer and Certain Pesticides for the Management of Brinjal Fruit Infection by Shoot Borer [Leucinodes Orbonalis (Guenée)] النص الكامل
2023
bhandari, Sagar | kandel, Bikash | gyawali, Pramod | pokhrel, Saurav | ojha, Sushil | bhandari, Sarita
The research aims to evaluate the efficacy of different levels of potassium fertilizer (8 g/plant K2O) as well as the insecticides neemix, spinosad, and imidacloprid at the rates of 6 ml, 0.33 ml, and 0.33 g/L water, respectively, for the management of brinjal fruits and shoot borer. The two-factor randomized complete block design with three replications was used. Significant minimum shoot damage was recorded in the plots treated with imidacloprid (0%), spinosad (1.67%) and neemix (5.0%) at 81 days after planting. Similarly, a significant minimum fruit infestation (2.90%) was recorded with imidacloprid, which was lower than those of spinosad (15.16%) and neemix (19.63%). The application of potassium fertilizer at a rate of 8 g/plant showed a significant reduction in shoot infestation (2.50%) 81 days after planting. The minimum percentage yield of infested fruit was reported with spinosad (1.03%), which was at par with other insecticidal treatments. Considering the negative impacts of chemical pesticides, neemix 6 ml/L water in combination with potassium fertilizer at 8 g/plant is recommended.
اظهر المزيد [+] اقل [-]Efficacy of Potassium Fertilizer and Certain Pesticides for the Management of Brinjal Fruit Infection by Shoot Borer [Leucinodes Orbonalis (Guenée)] النص الكامل
2023
Sagar bhandari | Bikash kandel | Pramod gyawali | Saurav pokhrel | Sushil ojha | Sarita bhandari
The research aims to evaluate the efficacy of different levels of potassium fertilizer (8 g/plant K2O) as well as the insecticides neemix, spinosad, and imidacloprid at the rates of 6 ml, 0.33 ml, and 0.33 g/L water, respectively, for the management of brinjal fruits and shoot borer. The two-factor randomized complete block design with three replications was used. Significant minimum shoot damage was recorded in the plots treated with imidacloprid (0%), spinosad (1.67%) and neemix (5.0%) at 81 days after planting. Similarly, a significant minimum fruit infestation (2.90%) was recorded with imidacloprid, which was lower than those of spinosad (15.16%) and neemix (19.63%). The application of potassium fertilizer at a rate of 8 g/plant showed a significant reduction in shoot infestation (2.50%) 81 days after planting. The minimum percentage yield of infested fruit was reported with spinosad (1.03%), which was at par with other insecticidal treatments. Considering the negative impacts of chemical pesticides, neemix 6 ml/L water in combination with potassium fertilizer at 8 g/plant is recommended.
اظهر المزيد [+] اقل [-]Evaluation of Biologically Treated Olive Mill Wastewater for Irrigation of Pea Plant النص الكامل
2023
Abdelhafez, Ahmed | yehia, Rokia | Abdelaal, Azza
Evaluation of Biologically Treated Olive Mill Wastewater for Irrigation of Pea Plant النص الكامل
2023
Abdelhafez, Ahmed | yehia, Rokia | Abdelaal, Azza
This study evaluated the use of biologically treated olive mill wastewater (OMWW) for irrigation of pea plants, rather than discharging this nutrient-rich liquid and polluting the environment. Pea seeds were planted in pots containing soil irrigated with tap water (control), untreated (crude) OMWW, or OMWW treated with the fungus, Pleurotus columbinus, or algae Spirulina platensis or Wollea sp., with two NPK rates. Plant length shoot and root dry weight, nitrogen, phosphorus, potassium, chlorophyll, and carotene contents were measured, along with to nitrogenase and dehydrogenase activity. The highest shoots N and P content were recorded in plants irrigated with Sp. Platensis-treated OMWW + 100% NPK; while the highest K content was in plants irrigated with crude OMWW + 100% NPK. The highest dehydrogenase activity, 59.01 μg TPF/100 g soils, was recorded in plants irrigated with P. columbinus-treated OMWW supported with 75% NPK, while maximum nitrogenase activity (261.82 μmol/100g soil/day) occurred in plants irrigated with Wollea sp.-treated OMWW with 75% NPK. The highest content of chlorophylls a & b and carotene (0.838, 0.276, 0.252 mg/g dry weight, respectively) were found in plants irrigated with OMWW treated with Wollea sp. and 100% NPK. Thus, biologically treated OMWW showed promising impacts on plant growth parameters.
اظهر المزيد [+] اقل [-]Evaluation of Biologically Treated Olive Mill Wastewater for Irrigation of Pea Plant النص الكامل
2023
Rokia yehia | Azza Abdelaal | Ahmed Abdelhafez
This study evaluated the use of biologically treated olive mill wastewater (OMWW) for irrigation of pea plants, rather than discharging this nutrient-rich liquid and polluting the environment. Pea seeds were planted in pots containing soil irrigated with tap water (control), untreated (crude) OMWW, or OMWW treated with the fungus, Pleurotus columbinus, or algae Spirulina platensis or Wollea sp., with two NPK rates. Plant length shoot and root dry weight, nitrogen, phosphorus, potassium, chlorophyll, and carotene contents were measured, along with to nitrogenase and dehydrogenase activity. The highest shoots' N and P contents were recorded in plants irrigated with Sp. platensis- treated OMWW + 100% NPK; while the highest K content was in plants irrigated with crude OMWW + 100% NPK. The highest dehydrogenase activity, 59.01 μg TPF/100 g soils, was recorded in plants irrigated with P. columbinus-treated OMWW supported with 75% NPK, while maximum nitrogenase activity (261.82 μmol/100g soil/day) occurred in plants irrigated with Wollea sp.-treated OMWW with 75% NPK. The highest content of chlorophylls a & b and carotene (0.838, 0.276, 0.252 mg/g dry weight, respectively) were found in plants irrigated with OMWW treated with Wollea sp. and 100% NPK. Thus, biologically-treated OMWW showed promising impacts on plant growth parameters.
اظهر المزيد [+] اقل [-]Effect of Foliar Spraying with Pink Pigmented Facultative Methylotrophic Bacteria on the Growth and Productivity of Strawberry النص الكامل
2023
Ismail, shadia | Mohammed, Fafy
Effect of Foliar Spraying with Pink Pigmented Facultative Methylotrophic Bacteria on the Growth and Productivity of Strawberry النص الكامل
2023
Ismail, shadia | Mohammed, Fafy
Pink-pigmented facultative methylotrophic bacteria (PPFM) isolated from cotton leaves was identified based on phenotypic and genotypic characteristics as Methylobacterium rodiotolerance. Two field experiments were conducted to investigate the effects of PPFM, methanol (10 and 30%) and a combination of bacteria and methanol on the growth, fruit quality, and yield of two strawberry cultivars (Florida and Festival). The main differences between the two cultivars are greater foliage fresh weight and early yield in cv. Florida while Festival cv. had a higher total yield per plant and greater anthocyanins and ascorbic acid contents. The greatest vegetative growth, dry matter percentage, potassium content, and carbohydrate content besides the earliest yield per plant were observed following spraying with PPFM or PPFM mixed with 10% methanol. Spraying with PPFM resulted in the highest total yield per plant, highest yield per feddan, and fruit quality. Spraying cv. Florida with PPFM resulted in the best early yield while spraying cv. Festival with PPFM resulted in the highest total yield and fruit quality. Spraying with PPFM appears to be the most efficient treatment for enhancing the total yield of Festival cv. by an average of 23.02 and 24.06 tons per feddan in the first and second seasons, respectively.
اظهر المزيد [+] اقل [-]Effect of Foliar Spraying with Pink Pigmented Facultative Methylotrophic Bacteria on the Growth and Productivity of Strawberry النص الكامل
2023
shadia Ismail | Fafy Mohammed
Pink-pigmented facultative methylotrophic bacteria (PPFM) isolated from cotton leaves was identified based on phenotypic and genotypic characteristics as Methylobacterium rodiotolerance. Two field experiments were conducted to investigate the effects of PPFM, methanol (10 and 30%) and a combination of bacteria and methanol on the growth, fruit quality, and yield of two strawberry cultivars (Florida and Festival). The main differences between the two cultivars are greater foliage fresh weight and early yield in cv. Florida while Festival cv. had a higher total yield per plant and greater anthocyanins and ascorbic acid contents. The greatest vegetative growth, dry matter percentage, potassium content, and carbohydrate content besides the earliest yield per plant were observed following spraying with PPFM or PPFM mixed with 10% methanol. Spraying with PPFM resulted in the highest total yield per plant, highest yield per feddan, and fruit quality. Spraying cv. Florida with PPFM resulted in the best early yield while spraying cv. Festival with PPFM resulted in the highest total yield and fruit quality. Spraying with PPFM appears to be the most efficient treatment for enhancing the total yield of Festival cv. by an average of 23.02 and 24.06 tons per feddan in the first and second seasons, respectively.
اظهر المزيد [+] اقل [-]Molecular Identification of Some Rhizobium and Serratia Isolates as Potential Producers of Indole-3-Acetic Acid النص الكامل
2023
Sello, Shaimaa | Abdel Razik, Ashraf | Ibrahim, Samir | Elateek, Sawsan
Molecular Identification of Some Rhizobium and Serratia Isolates as Potential Producers of Indole-3-Acetic Acid النص الكامل
2023
Sello, Shaimaa | Abdel Razik, Ashraf | Ibrahim, Samir | Elateek, Sawsan
In order to reduce the use of chemical fertilizers, the use of biological inoculants has been increased. Beneficial microorganisms are used to increase crop yields by stimulating plant growth through the production of phytohormones. In the present study, Indole Acetic Acid (IAA) production was analyzed in 18 bacterial isolates with Plant Growth-Promoting (PGP) capabilities; 15 of these isolates were Rhizobium, and three were Serratia. All isolates have been characterized morphologically and biochemically, and their IAA production in the presence of tryptophan, a precursor for IAA biosynthesis, has been evaluated. IAA production was detected using the Salkowski reagent with CT-2200 spectrophotometer at 530 nm. The levels of IAA production varied between the different isolates. The top two IAA producers were selected for genetic identification using 16SrRNA primers (27F and 1492R). One of the Rhizobium isolates (NRC-R2) shared 95% sequence similarity with Rhizobium sp., according to a Blastn search in GenBank and Rhizobium leguminosarum, whereas the Serratia isolate (Ain Shams Center for Genetic Engineering and Biotechnology (ACGEB)-S2 was 99.5% similar to Serratia sp. In the near future, it is possible that these two isolates could serve as biological sources for IAA.
اظهر المزيد [+] اقل [-]Molecular Identification of Some Rhizobium and Serratia Isolates as Potential Producers of Indole-3-Acetic Acid النص الكامل
2023
Shaimaa Sello | Ashraf Abdel Razik | Samir Ibrahim | Sawsan Elateek
In order to reduce the use of chemical fertilizers, the use of biological inoculants has been increased. Beneficial microorganisms are used to increase crop yields by stimulating plant growth through the production of phytohormones. In the present study, Indole Acetic Acid (IAA) production was analyzed in 18 bacterial isolates with Plant Growth-Promoting (PGP) capabilities; 15 of these isolates were Rhizobium, and three were Serratia. All isolates have been characterized morphologically and biochemically, and their IAA production in the presence of tryptophan, a precursor for IAA biosynthesis, has been evaluated. IAA production was detected using the Salkowski reagent with CT-2200 spectrophotometer at 530 nm. The levels of IAA production varied between the different isolates. The top two IAA producers were selected for genetic identification using 16SrRNA primers (27F and 1492R). One of the Rhizobium isolates (NRC-R2) shared 95% sequence similarity with Rhizobium sp., according to a Blastn search in GenBank and Rhizobium leguminosarum, whereas the Serratia isolate (Ain Shams Center for Genetic Engineering and Biotechnology (ACGEB)-S2 was 99.5% similar to Serratia sp. Accordingly, these two isolates could serve as biological sources for IAA.
اظهر المزيد [+] اقل [-]Residue Assessment of Bifenazate, Spirodiclofen and Abamectin in Strawberry Fruits Under Field Conditions النص الكامل
2023
Abdelmaksoud, Eman | Elsayed, Walaa | Elrefai, Shoukry | mahmoud, kadry
Residue Assessment of Bifenazate, Spirodiclofen and Abamectin in Strawberry Fruits Under Field Conditions النص الكامل
2023
Abdelmaksoud, Eman | Elsayed, Walaa | Elrefai, Shoukry | mahmoud, kadry
The dissipation behavior of three pesticides, bifenazate, spirodiclofen and abamectin was studied in strawberry fruits using a modified QuEChERS extraction technique. The residues of the tested acaricides were determined under field conditions after 1 hour (zero days), 1, 3, 7, 14, and 21 days. The maximum residue limits (MRLs) were 2, 2, and 0.15 mg/kg for bifenazate, spirodiclofen and abamectin, respectively. The strawberry fruits could be used safely after 15 days from bifenazate spraying and after 3 days from spirodiclofen spraying. The concentration of abamectin after 21 days did not reach MRL. The half-life values (t1/2) of bifenazate, spirodiclofen, and abamectin in strawberry fruits were 0.99, 0.86, and 5.7 days, respectively.
اظهر المزيد [+] اقل [-]Residue Assessment of Bifenazate, Spirodiclofen and Abamectin in Strawberry Fruits Under Field Conditions النص الكامل
2023
Eman Abdelmaksoud | Walaa Elsayed | Shoukry Elrefai | kadry mahmoud | Khaled Ramadan
The dissipation behavior of three pesticides, bifenazate, spirodiclofen and abamectin was studied in strawberry fruits using a modified QuEChERS extraction technique. The residues of the tested acaricides were determined under field conditions after 1 hour (zero days), 1, 3, 7, 14, and 21 days. The maximum residue limits (MRLs) were 2, 2, and 0.15 mg/kg for bifenazate, spirodiclofen and abamectin, respectively. The strawberry fruits could be used safely after 15 days from bifenazate spraying and after 3 days from spirodiclofen spraying. The concentration of abamectin after 21 days did not reach MRL. The half-life values (t1/2) of bifenazate, spirodiclofen, and abamectin in strawberry fruits were 0.99, 0.86, and 5.7 days, respectively.
اظهر المزيد [+] اقل [-]