Feline calicivirus (FCV) is a highly infectious respiratory pathogen of domestic cats with a widespread distribution. In order to assess how FCV circulates in feral and household cats, we have carried out the first study on the FCV detection in Ahvaz district from December 2008 to November 2009. Oropharyngeal, nasal and ocular swabs of one hundred cats (70 feral and 30 household) were evaluated by reverse transcription polymerase chain reaction (RT-PCR) procedure for the detection of FCV. The influence of sex, age, social status and clinical signs on the probability of infection was analyzed using statistical Fisher’s exact test. Overall, feline calicivirus was detected in 4/100 (4%) of sampled cats; 13.3% of the household cats were FCV positive compared to 0% of feral cats. According to several factors including younger cats (under 6 months of age), multiple cat household and clinical findings, differences were significant (p0.05). To the best of our knowledge, this is the first report indicating the presence of FCV in cats in Iran. Due to low prevalence of FCV infection and the fact that feral cats live solitarily, it was concluded that this viral infection don’t spread readily within feral populations. However special measures are recommended to avoid infection of susceptible and unvaccinated cats.

This study was conducted to evaluate the effect of dietary melatonin supplementation (10 mg/kg body wt. for 4 weeks) on some erythrocytic antioxidant enzymes and lipid peroxidation as well as some plasma biochemical parameters in common carp. The results showed that the activities of glutathione peroxidase and superoxide dismutase were increased significantly (p < 0.05) after melatonin supplementation, whereas alterations in catalase activity were not significant. Malondildehyde concentrations in erythrocyte hemolysate were decreased significantly as a result of melatonin treatment. Plasma biochemical analysis revealed a significant decrease of glucose, cholesterol, albumin and triglyceride, in addition to a significant increase in total protein, AST and ALP values when compared to the control group. However, alterations in creatinine, urea and LDH levels were not significant following melatonin supplementation compared to the control values. These results offer that pharmacological amounts of melatonin effectively improve erythrocytic oxidative status biomarkers and also alter some plasma biochemical parameters in the common carp that some of them may be undesirable. However, more investigations are required to elucidate the pharmacokinetic effects of melatonin and also possible undesirable effects of high doses of this compound.

This study was conducted to determine the effects of administration of different concentration of Aloe vera Crud Extract (ACE) as a food supplement on growth performance, immune response and disease resistance against Aeromonas hydrophila (Chester) infection in Cyprinus carpio. 360 juvenile C.carpio were randomly divided into four equal groups in triplicates. Groups 1 to 4 were fed by basal food supplemented with zero, 0.1, 0.5 and 1% ACE respectively, for 60 days. At the end of the experimental period growth performance indices including: SpecificGrowth Rate (SGR), Food Conversion Rate (FCR), Food Efficacy Rate (FER) and Percentage Weight-Gain (PWG) were evaluated. Blood samples were taken from each treatment and Haematological parameters including: Red Blood Cells (RBC) & White Blood Cell(WBC) count, differential count, Hemoglobin (Hb), Hematocrit (Hct), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH) and the Mean Corpuscular Hemoglobin Concentration (MCHC) as well as Immunological parameters including: lysozyme activity, serum bactericidal activity, alternative complement activity, respiratory burst activity, serum total protein and globulin were evaluated. Finally challenge with Aeromonas hydrophila was performed and commutative mortality was calculated. Results showed significant improve in growth indices of groups 3 and 4 (p

The aim of this study was to investigate the histopathology of avian tuberculosis in naturally infected domestic pigeons (Columba livia var. domestica) with Mycobacterium aviumsubsp.avium. Avian tuberculosis is one of the most important diseases that affect all species of birds, and is most often caused by mycobacterium avium and mycobacterium genavense. Eighty out of more than 600 pigeons were selected based on their clinical signs and poor healthconditions and under standard conditions were euthanized, necropsied, and followed by bacterial culture on specific media for Mycobacterium avium subsp.avium. Fifty Mycobacterium avium subsp. Avium was isolated from pigeons. All acid fast bacilli isolates were tested by the PCR assays targeting the 16S rRNA, IS1245 and IS901 genes. After definitive identification of Mycobacterium aviumsubsp. avium by culturing and PCR assay, 45 fixed samples including liver, gizzard, proventriculus, intestines, kidneys and lungs from positive pigeons were subjected for histopathology studies. Tissues sections were prepared as usual and stained by hematoxylin and eosin, Ziehl-Neelsen and Congo red. Based on gross findings, liver and intestines were the most affected organs. Histologically, caseative uncalcified granulomatous inflammation was noted in the affected organs. Also histopathology examinations showed that most of the granulomatous lesions in the lungs were in microscopic size and it seems that lungs were affected more than it was expected. In Ziehl-Neelsen’s staining, a large number of acid-fast bacilli were observed within multinucleated giant cells and in necrotic areas. Also in Congo red staining, deposition of amyloid in liver and kidneys sections were observed. In conclusion, histopathology findings were typical of avian tuberculosis, including acid fast bacilli and uncalcified caseous necrosis centers which were surrounded by multinucleated giant cells, macrophages and lymphocytes.

Equine babesiosis is a hemoprotozoan tickborne disease with worldwide distribution and caused by Theileria equi and Babesia caballi. This study was conducted to determine the seroprevalence of T. equi and B. caballi infection in Turkoman breed horses in North Khorasan Province of Iran. Blood samples were collected from 100 apparently healthy horses and examined by microscopy and indirect immunofluorescent antibody test. T. equi was microscopically detected in five blood smears. Antibodies against T. equi, B. caballi and dual infection were found in 48 (48%), 2 (2%) and 3 (3%) serum samples, respectively. No significant difference was observed between the seroprevalence of piroplasm infection with risk factors such as age, gender and activity in horses. This is the first report of detection of T. equi and B. caballi infection using IFAT in Iran. It was concluded that the seroprevalence of T. equi infection is higher than B. caballi infection in Turkoman breed horse in Iran.

Atrial fibrillation (AF) was detected as an incidental finding in a 14 years old brood mare horse which was maintained for student education in the teaching hospital, Faculty of Veterinary Medicine, Mashhad. Irregular heart rhythm, variable intensity of heart sounds and "f" waves were revealed in clinical examination and electrocardiography. Hematology showed normal values and hyponutremia was observed in serum biochemical analysis. Treatment of the horse was done with oral administration of uinidine sulfate (200 mg tabs). Normal rhythm was appeared after 24 hours of treatment. Herein, we have discussed the etiology and the treatment procedure of this dysrhythmia.

Platelets are a natural source of growth factors and cytokines that promote wound healing, and platelet-rich fibrin contains concentrated growth factors. Growth factors in platelet-rich fibrin may promote wound repair. In this study the effect of platelet-rich fibrin was evaluated in a dog model of cutaneous incisional wounds using histology and semi-quantitative evaluation. A pair of ten-centimeter full-thickness parallel linear cutaneous wounds were created on the backs of 15 dogs on the both sides of vertebral column. On the left side the platelet-rich fibrin clot was applied to the edges of the wound (Treatment group) and the right side received nothing (Control group). All wounds were then closed with 3-0 non-absorbable Nylon suture. The dogs were divided into three groups of five dogs. The wound tissues were sampled by electrosurgery in group one after 3 days, group two after 7 days and group three after 14 days post surgery. For each specimen, histopathological examination and semi-quantitative evaluation was performed by light microscopy using Hematoxylin & Eosin and Masson’s trichrome staining. The results demonstrated that platelet-rich fibrin improved and accelerated cutaneous incisional wound healing.

Bovine Ephemeral Fever (BEF) flared up in a dairy farm with 2097 animals. The disease started in September, 2006, with daily means of environmental temperature (ET) and relative humidity (RH) of 23.8 ºC and 37%, respectively, and ended after 48 days with ET and RH of 16.2 ºC and 68%, respectively. In this outbreak, the age of affected animals was above 10 months and the morbidity rate was 13.07%. Clinical signs included fever, hyperpnoea, mouth breathing, subcutaneous emphysema and death. Histologically, there were vasculitis,hyperemia; hemorrhage and edema in soft tissues and rupture of alveolar walls. Both Culex and Colicoides spp. were captured as vectors. Bovine Ephemeral Fever virus genome was detected in blood samples by RT-PCR and the CPE was shown by blood sample culture.

Aseel is an indigenous breed of poultry recognized for its majestic gait and cock fighting.Southern part of Chhattisgarh is its breeding tract. Vanaraja is a crossbreed being popularized by the Department of Animal Husbandary (C.G.) to improve the livelihood of tribal peoples.Ductus deferens is part of reproductive system which plays an important role in transportation of spermatozoa and fertilization. In the present study, 10 birds of two age group viz. 5 months (grower) and 13 months (adult) of each breed were used. Ductus deferens was collected,processed and sections were stained for demonstration of normal histological structure, collagen fibers, elastic fibers, reticular fibers, carbohydrates and mucopolysaccharides. The height of epithelium, height of mucosal folds at different segments, number of mucosal folds per transverse section, thickness of wall excluding mucosal folds, maximum and minimum diameter of ductus deferens was significantly higher in growers and adults of Vanaraja than Aseel. The density of connective tissue fibers, PAS activity and AB-PAS activity was higher in both groups of Vanaraja compared to Aseel.

Cobalt is an essential co-factor in red blood cell production and function and its deficiency may produce clinical signs in sheep. Thus, present study was designed to evaluate the effect of cobalt nano-particles on serum biochemical factors and histopathological changes in liver and kidnies of lambs. Study was carried out in 3 groups of lambs (4 lambs per group). One group of lambs was kept as control group. Second and third group respectively received cobalt nano-particles and conventional cobalt chloride suspension daily for a period 25 day. Blood sample and then serum was collected before and at the end of study. Activity of ALT,AST, ALP and level of BUN, creatinine and vitamin B12 were measured in serum of lambs. Tissue sections of liver and kidney were stained with hematoxylin and eosin and examined by light microscopy. Activity of ALT, BUN and vitamin B12 was significantly increased by cobalt nano-particles and conventional cobalt chloride. Fatty change of hepatocytes occured by conventional cobalt and granulomatous hepatitis, focal necrosis of hepatocytes and degeneration of hepatocytes by the nano cobalt was identified in liver. There were not any significant lesions and alteration in the kidnies of treated groups. Thus, cobalt nano-particles have similar effect to conventional cobalt for using in sheep with cobalt deficiency.