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Bovine dermatophilosis: Awareness, perceptions and attitudes in the small-holder sector of north-west Zimbabwe
2016
Ndhlovu, Daud N.(University of Zimbabwe Department of Clinical Veterinary Studies) | Masika, Patrick J.(Fort Cox College of Agriculture and Forestry)
A cross-sectional study was conducted to assess cattle owners' awareness, perceptions, attitudes and drug-usage practices with regard to bovine dermatophilosis. Knowledge of these farmers' attributes is important for animal health policy makers in their endeavours to provide optimum disease control strategies that are acceptable to the communities. Data on cattle owner awareness of bovine dermatophilosis, causes, treatment practices, perceptions about its importance and potential dangers to humans were collected using an interviewer-administered questionnaire. A total of 185 stockowners and cattle herds were involved in the study, with bovine dermatophilosis determined clinically by veterinarians. The results showed that 45.4% of the herds were clinically positive for dermatophilosis, and most farmers (79.5%) were generally aware that dermatophilosis was a cattle disease. In the event of a dermatophilosis outbreak in a herd, 74.1% of the farmers treated their cattle using antibiotics; the proportion of farmers treating cattle did not differ (p > 0.05) across the diptanks. Fifty-two farmers (52/63) indicated that drugs had to be administered four to seven times before an animal recovered from infection. Tetracyclines were the antibiotics used by most farmers (79.3%) to treat dermatophilosis, with 19.1% using penicillins. Concerns were raised by farmers about the effectiveness of these drugs against bovine dermatophilosis. Across the study sites, 48.6% and 27.6% of the farmers perceived bovine dermatophilosis to be an important disease at the herd and area level, respectively. A small proportion (12.4%) of the farmers regarded bovine dermatophilosis as a potentially zoonotic disease. The high level of stockowners' general awareness, with regards to bovine dermatophilosis, sets ideal conditions for the mobilisation of farmers by animal health authorities in the control of the disease. However, further research needs to be undertaken to investigate effective antibiotic delivery protocols and the potential zoonotic impact of bovine dermatophilosis in a situation of high disease prevalence.
اظهر المزيد [+] اقل [-]Medicinal plants used to control internal and external parasites in goats
2016
Sanhokwe, Marcia(University of Fort Hare Alice Department of Livestock and Pasture Science) | Mupangwa, Johnfisher(University of Fort Hare Alice Department of Livestock and Pasture Science) | Masika, Patrick J.(University of Fort Hare Alice Department of Livestock and Pasture Science ,Fort Cox College of Agriculture and Forestry) | Maphosa, Viola(University of Fort Hare Alice Department of Livestock and Pasture Science) | Muchenje, Voster(University of Fort Hare Alice Department of Livestock and Pasture Science)
The use of medicinal plants plays a major role in the primary health care of animals in South Africa. A survey was conducted to document medicinal plants used to control parasites in goats in Kwezi and Ntambethemba villages in the Eastern Cape Province, South Africa. Information from 50 farmers and 3 herbalists was obtained through the use of a structured questionnaire, and a snowball sampling technique was used to identify key informants. The obtained data were analysed using PROC FREQ of SAS (2003), and fidelity level values were determined to estimate the healing potential of the mentioned plants. The survey revealed nine plant species belonging to eight families that were used to control parasites in goats. Asphodelaceae (22.22%) was the most frequently used plant family. Leaves were the most used plant parts, constituting 60.38%. They were prepared either as infusions or decoctions of single plants or in mixtures. Aloe ferox, Acokanthera oppositifolia and Elephantorrhiza elephantina were the plants having the highest fidelity level for their use to control parasites, each scoring 100%, followed by Albuca setosa (83.33%). The study revealed low knowledge about ethno-veterinary medicine in the study area. It also revealed that information on ethno-veterinary medicine in this area is mostly confined to older people and there is danger that this knowledge can be lost before being passed on to other generations. Therefore, there is an urgent need to document information on these plant species so that the future generation can benefit. Further investigation should be carried out to validate the efficacy and safety of the above-mentioned plants so as to provide cheap alternative ways of controlling parasites.
اظهر المزيد [+] اقل [-]Diagnosis and genotyping of African swine fever viruses from 2015 outbreaks in Zambia
2016
Thoromo, Jonas(Sokoine University of Agriculture Department of Veterinary Microbiology and Parasitology) | Simulundu, Edgar(The University of Zambia Department of Disease Control) | Chambaro, Herman M.(Ministry of Fisheries and Livestock) | Mataa, Liywalii(Ministry of Fisheries and Livestock) | Lubaba, Caesar H.(Ministry of Fisheries and Livestock) | Pandey, Girja S.(The University of Zambia Department of Disease Control) | Takada, Ayato(Hokkaido University Research Center for Zoonosis Control Division of Global Epidemiology) | Misinzo, Gerald(Sokoine University of Agriculture Department of Veterinary Microbiology and Parasitology) | Mweene, Aaron S.(The University of Zambia Department of Disease Control)
In early 2015, a highly fatal haemorrhagic disease of domestic pigs resembling African swine fever (ASF) occurred in North Western, Copperbelt, and Lusaka provinces of Zambia. Molecular diagnosis by polymerase chain reaction targeting specific amplification of p72 (B646L) gene of ASF virus (ASFV) was conducted. Fourteen out of 16 domestic pigs from the affected provinces were found to be positive for ASFV. Phylogenetic analyses based on part of the p72 and the complete p54 (E183L) genes revealed that all the ASFVs detected belonged to genotypes I and Id, respectively. Additionally, epidemiological data suggest that the same ASFV spread from Lusaka to other provinces possibly through uncontrolled and/or illegal pig movements. Although the origin of the ASFV that caused outbreaks in domestic pigs in Zambia could not be ascertained, it appears likely that the virus may have emerged from within the country or region, probably from a sylvatic cycle. It is recommended that surveillance of ASF, strict biosecurity, and quarantine measures be imposed in order to prevent further spread and emergence of new ASF outbreaks in Zambia.
اظهر المزيد [+] اقل [-]Sero-epidemiological survey and risk factors associated with bovine brucellosis among slaughtered cattle in Nigeria
2016
Akinseye, Victor O.(University of Ibadan Department of Veterinary Public Health and Preventive Medicine) | Adesokan, Hezekiah K.(University of Ibadan Department of Veterinary Public Health and Preventive Medicine) | Ogugua, Akwoba J.(University of Ibadan Department of Veterinary Public Health and Preventive Medicine) | Adedoyin, Folashade J.(University of Ibadan Department of Veterinary Public Health and Preventive Medicine) | Otu, Patricia I.(University of Ibadan Department of Veterinary Public Health and Preventive Medicine) | Kwaghe, Ayi V.(University of Maiduguri Department of Veterinary Medicine) | Kolawole, Noah O.(University of Ibadan Department of Veterinary Public Health and Preventive Medicine) | Okoro, Oyinye J.(University of Nigeria Department of Veterinary Public Health and Preventive Medicine) | Agada, Charity A.(University of Agriculture Department of Veterinary Public Health and Preventive Medicine) | Tade, Adeniyi O.(Federal University of Agriculture Department of Veterinary Public Health and Reproduction) | Faleke, Olufemi O.(Uthman dan Fodiyo University Department of Veterinary Public Health and Preventive Medicine) | Okeke, Anyanwu L.(National Veterinary Research Institute Vom) | Akanbi, Ibikunle M.(Ministry of Agriculture and Rural Developmen Department of Veterinary Services) | Ibitoye, Mofoluwake M.(Ministry of Agriculture and Rural Developmen Department of Veterinary Services) | Dipeolu, Morenike O.(Federal University of Agriculture Department of Veterinary Public Health and Reproduction) | Dale, Emma J.(Animal & Plant Health Agency Department of Bacteriology and TB) | Lorraine, Perrett(Animal & Plant Health Agency Department of Bacteriology and TB) | Taylor, Andrew V.(Animal & Plant Health Agency Department of Bacteriology and TB) | Awosanya, Emmanuel A.(University of Ibadan Department of Veterinary Public Health and Preventive Medicine) | Cadmus, Eniola O.(University of Ibadan Department of Preventive Medicine and Primary Care) | Stack, Judy A.(Animal & Plant Health Agency Department of Bacteriology and TB) | Cadmus, Simeon I.(University of Ibadan Department of Veterinary Public Health and Preventive Medicine)
Bovine brucellosis is endemic in Nigeria; however, limited data exist on nationwide studies and risk factors associated with the disease. Using a cross-sectional sero-epidemiological survey, we determined the prevalence of and risk factors for brucellosis in slaughtered cattle in three geographical regions of Nigeria. Serum samples from randomly selected unvaccinated cattle slaughtered over a period of 3 years (between December 2010 and September 2013) from northern, southern and south-western Nigeria were tested for antibodies to Brucella abortus using the Rose Bengal test. Data associated with risk factors of brucellosis were analysed by Stata Version 12. In all, 8105 cattle were screened. An overall seroprevalence of 3.9% (315/8105) was recorded by the Rose Bengal test, with 3.8%, 3.4% and 4.0% from the northern, southern and south-western regions, respectively. Bivariate analysis showed that cattle screened in northern Nigeria were less likely to be seropositive for antibodies to Brucella spp. than those from south-western Nigeria (odds ratio = 0.94; 95% confidence interval: 0.73-1.22). However, logistic regression analysis revealed that breed (p = 0.04) and sex (p £ 0.0001) of cattle were statistically significant for seropositivity to Brucella spp. The study found that brucellosis was endemic at a low prevalence among slaughtered cattle in Nigeria, with sex and breed of cattle being significant risk factors. Considering the public health implications of brucellosis, we advocate coordinated surveillance for the disease among diverse cattle populations in Nigeria, as is carried out in most developed countries.
اظهر المزيد [+] اقل [-]The African swine fever control zone in South Africa and its current relevance
2016
Magadla, Noluvuyo R.(Department of Agriculture and Rural Development ,University of Pretoria Department of Production Animal Studies) | Vosloo, Wilna(CSIRO-Australian Animal Health Laboratory) | Heath, Livio(Onderstepoort Veterinary Institute Agricultural Research Council) | Gummow, Bruce(University of Pretoria Department of Production Animal Studies ,James Cook University)
African swine fever (ASF) has been reported in South Africa since the early 20th century. The disease has been controlled and confined to northern South Africa over the past 80 years by means of a well-defined boundary line, with strict control measures and movement restrictions north of this line. In 2012, the first outbreak of ASF outside the ASF control zone since 1996 occurred. The objective of this study was to evaluate the current relevance of the ASF control line as a demarcation line between endemic ASF (north) areas and ASF-free (south) area and to determine whether there was a need to realign its trajectory, given the recent outbreaks of ASF, global climate changes and urban development since the line's inception. A study of ASF determinants was conducted in an area 20 km north and 20 km south of the ASF control line, in Limpopo, Mpumalanga, North West and Gauteng provinces between May 2008 and September 2012. The study confirmed that warthogs, warthog burrows and the soft tick reservoir, Ornithodoros moubata, are present south of the ASF control line, but no virus or viral DNA was detected in these ticks. There appears to be an increasing trend in the diurnal maximum temperature and a decrease in humidity along the line, but the impact of these changes is uncertain. No discernible changes in minimum temperatures and average rainfall along the disease control line were observed between 1992 and 2014. Even though the reservoirs were found south of the ASF boundary line, the study concluded that there was no need to realign the trajectory of the ASF disease control line, with the exception of Limpopo Province. However, the provincial surveillance programmes for the reservoir, vector and ASF virus south of this line needs to be maintained and intensified as changing farming practices may favour the spread of ASF virus beyond the control line.
اظهر المزيد [+] اقل [-]Salmonella contamination, serovars and antimicrobial resistance profiles of cattle slaughtered in South Africa
2016
Madoroba, Evelyn(Agricultural Research Council-Onderstepoort Veterinary Institute ,University of South Africa College of Agriculture and Environmental Sciences) | Kapeta, Daniel(University of South Africa College of Agriculture and Environmental Sciences) | Gelaw, Awoke K.(Agricultural Research Council-Onderstepoort Veterinary Institute)
Antimicrobial resistant Salmonella are among the leading causes of foodborne infections. Our aim was to determine Salmonella contamination during cattle slaughter in South African rural abattoirs (n = 23) and environmental samples. Furthermore, antimicrobial resistance patterns of the Salmonella isolates were determined. Samples of cattle faeces (n = 400), carcass sponges (n = 100), intestinal contents (n = 62), hides (n = 67), and water from the abattoirs (n = 75) were investigated for Salmonella species using microbiological techniques and species-specific polymerase chain reaction targeting the invA gene. In total 92 Salmonella species isolates were recovered. The Salmonella mean frequency of occurrence on hides, carcasses, and intestinal contents was 35.37% (n = 81). Eleven faecal samples (2.75%) tested positive for Salmonella. The predominant serovar was Salmonella Enteritidis. Diverse serovars that were identified on carcasses were not necessarily found on the hides and intestinal contents. The inconsistent occurrence of the diverse Salmonella serovars on hides, carcasses, and intestinal contents implies that in addition to carriage on hides and in intestinal contents, other external factors also play an important role regarding carcass contamination. The 92 Salmonella were serotyped and tested for susceptibility towards the following antimicrobials: ampicillin, cefotaxime, enrofloxacin, kanamycin, and oxytetracycline using the disk diffusion method. Most Salmonella (n = 66; 71.7%) isolates were resistant to at least one antimicrobial with highest resistance observed towards oxytetracycline (51.90%), which highlights the need for strict hygiene during slaughter and prudent antimicrobial use during animal production. In conclusion, cattle slaughtered in South African rural abattoirs harbour diverse Salmonella serovars that are resistant to antimicrobials, which could be a public health risk. The findings should assist policymakers with improving implementation of hygienic slaughter of cattle in rural abattoirs, which is paramount from socioeconomic, public health, and epidemiological standpoints.
اظهر المزيد [+] اقل [-]An update of the tsetse fly (Diptera: Glossinidae) distribution and African animal trypanosomosis prevalence in north-eastern KwaZulu-Natal, South Africa
2016
de Beer, Chantel J.(Onderstepoort Veterinary Institute ,University of the Free State Department of Zoology and Entomology) | Venter, Gert J.(Onderstepoort Veterinary Institute ,University of Pretoria Department of Veterinary and Tropical Diseases) | Green, Karin Kappmeier | Esterhuizen, Johan(Onderstepoort Veterinary Institute ,Liverpool School of Tropical Medicine Department of Vector Biology) | de Klerk, Daniel G.(Onderstepoort Veterinary Institute) | Ntshangase, Jerome(Onderstepoort Veterinary Institute) | Vreysen, Marc J.B.(Joint Food and Agriculture Organization) | Pienaar, Ronel(Onderstepoort Veterinary Institute) | Motloang, Makhosazana(Onderstepoort Veterinary Institute) | Ntantiso, Lundi(Makhathini Research Station) | Latif, Abdalla A.(Onderstepoort Veterinary Institute ,University of KwaZulu-Natal College of Agriculture, Engineering and Science)
An unpredicted outbreak of African animal trypanosomosis or nagana in 1990 in north-eastern KwaZulu-Natal necessitated an emergency control programme, utilising the extensive cattle-dipping system in the area, as well as a reassessment of the tsetse and trypanosomosis problem in the province. Since 1990, sporadic blood sampling of cattle at the dip tanks in the nagana-infested areas were undertaken to identify trypanosome species involved and to determine the infection prevalence in cattle. The distribution and species composition of the tsetse populations in the area were also investigated. From November 2005 to November 2007 selected dip tanks were surveyed for trypanosome infection prevalence. During April 2005 to August 2009 the distribution and abundance of tsetse populations were assessed with odour-baited H traps. The tsetse and trypanosome distribution maps were updated and potential correlations between tsetse apparent densities (ADs) and the prevalence of trypanosomosis were assessed. Glossina brevipalpis Newstead and Glossina austeni Newstead were recorded in locations where they have not previously been collected. No significant correlation between tsetse relative abundance and nagana prevalence was found, which indicated complex interactions between tsetse fly presence and disease prevalence. This was epitomised by data that indicated that despite large differences in the ADs of G. austeni and G. brevipalpis, trypanosome infection prevalence was similar in all three districts in the area. This study clearly indicated that both tsetse species play significant roles in trypanosome transmission and that it will be essential that any control strategy, which aims at sustainable management of the disease, should target both species.
اظهر المزيد [+] اقل [-]Preliminary report on osteochondrosis in cattle in the north-western parts of South Africa
2016
Prozesky, Leon(University of Pretoria Department of Paraclinical Sciences) | Neser, Johan(University of Pretoria Department of Paraclinical Sciences) | Meissner, Heinz(University of Pretoria Department of Paraclinical Sciences) | Botha, Kenneth(University of Pretoria Department of Paraclinical Sciences) | Jacobs, Lubbe(Lubern Animal Feeds) | Shepstone, Craig(University of Pretoria Department of Paraclinical Sciences) | Viljoen, Hannes(Animal Nutrition and Health) | Köster, Hinner(Kaonna Investments (Pty) Ltd) | de Brouwer, Chris | van Zyl, Jan | van der Veen, Gerjan
The north-western part of South Africa, in particular, is well known for mineral imbalances. Aphosphorosis, resulting in rickets and osteomalacia, received a lot of attention at the turn of the nineteenth century (1882-1912). This was followed in 1997 by research on Vryburg hepatosis, another area-specific mineral imbalance-related disease in young calves reared on manganese-rich soil derived from the weathering of dolomitic (carbonate) rock formations. In 1982, a totally new syndrome (osteochondrosis) manifested in, amongst others, areas in South Africa where aphosphorosis was rife. Osteochondrosis was also identified in the south-western parts of Namibia as well as southern Botswana and other areas in South Africa. Osteochondrosis has a multifactorial aetiology and this study focused on the role of minerals, particularly phosphorus, in the development of the disease. A significant improvement in the clinical signs in experimental animals and a reduction of osteochondrosis occurred on farms where animals received bioavailable trace minerals and phosphorus as part of a balanced lick. An increase in the occurrence of the disease on farms during severe drought conditions in 2012-2013 prompted researchers to investigate the possible role of chronic metabolic acidosis in the pathogenesis of the disease.
اظهر المزيد [+] اقل [-]Cellular immune responses induced in vitro by Ehrlichia ruminantium secreted proteins and identification of vaccine candidate peptides
2016
Thema, Nontobeko(Agricultural Research Council- Onderstepoort Veterinary Institute ,University of Pretoria Department of Veterinary Tropical Diseases) | Pretorius, Alri(Agricultural Research Council- Onderstepoort Veterinary Institute ,University of Pretoria Department of Veterinary Tropical Diseases) | Tshilwane, Selaelo I.(Agricultural Research Council- Onderstepoort Veterinary Institute) | Liebenberg, Junita(Agricultural Research Council- Onderstepoort Veterinary Institute) | Steyn, Helena(Agricultural Research Council- Onderstepoort Veterinary Institute) | van Kleef, Mirinda(Agricultural Research Council- Onderstepoort Veterinary Institute ,University of Pretoria Department of Veterinary Tropical Diseases)
Secreted proteins are reported to induce cell-mediated immunity characterised by the production of interferon-gamma (IFN)-γ. In this study three open reading frames (ORFs) (Erum8060, Erum7760, Erum5000) encoding secreted proteins were selected from the Ehrlichia ruminantium (Welgevonden) genome sequence using bioinformatics tools to determine whether they induce a cellular immune response in vitro with mononuclear cells from needle and tick infected animals. The whole recombinant protein of the three ORFs as well as four adjacent fragments of the Erum5000 protein (Erum5000A, Erum5000B, Erum5000C, Erum5000D) were successfully expressed in a bacterial expression system which was confirmed by immunoblots using anti-His antibodies and sheep sera. These recombinant proteins were assayed with immune sheep and cattle peripheral blood mononuclear cells (PBMCs), spleen and lymph node (LN) cells to determine whether they induce recall cellular immune responses in vitro. Significant proliferative responses and IFN-γ production were evident for all recombinant proteins, especially Erum5000A, in both ruminant species tested. Thus overlapping peptides spanning Erum5000A were synthesised and peptides that induce proliferation of memory CD4+ and CD8+ T cells and production of IFN-γ were identified. These results illustrate that a Th1 type immune response was elicited and these recombinant proteins and peptides may therefore be promising candidates for development of a heartwater vaccine.
اظهر المزيد [+] اقل [-]Effect of the infection with the nematode Haemonchus contortus (Strongylida: Trichostrongylidae) on the haematological, biochemical, clinical and reproductive traits in rams
2016
Rouatbi, Mariem(Manouba University Laboratory of Parasitology) | Gharbi, Mohamed(Manouba University Laboratory of Parasitology) | Rjeibi, Mohamed R.(Manouba University Laboratory of Parasitology) | Salem, Imen Ben(Manouba University Department of Animal Production, Service of Animal Science) | Akkari, Hafidh(Manouba University Laboratory of Parasitology) | Lassoued, Narjess(National Institute of Agronomic Research of Tunisia Department of Animal and Forage) | Rekik, Mourad(International Center for Agricultural Research in the Dry Areas)
This study aimed to investigate the effect of Haemonchus contortus infection on rams' haematological, biochemical and clinical parameters and reproductive performances. A total number of 12 Barbarine rams (control and infected) were included in the experiment. The infected group received 30 000 H. contortus third-stage larvae orally. Each ram's ejaculate was immediately evaluated for volume, sperm cell concentration and mortality rate. At the end of the experiment (day 82 post-infection), which lasted 89 days, serial blood samples were collected in order to assess plasma testosterone and luteinising hormone (LH) concentrations. There was an effect of time, infection and their interaction on haematological parameters (p < 0.001). In infected rams, haematocrit, red blood cell count and haemoglobin started to decrease from 21 days post-infection. There was an effect of time and infection for albumin. For total protein, only infection had a statistically significant effect. For glucose, only time had a statistically significant effect. Concentrations were significantly lower in infected rams compared to control animals. A significant effect of infection and time on sperm concentrations and sperm mortality was observed. The effect of infection appears in time for sperm concentrations at days 69 and 76 post-infection. Sperm mortality rate was significantly higher in infected animals at day 46 post-infection when compared to control group (p < 0.05). Finally, plasma testosterone traits (average concentration, cumulated levels during the sampling period and pulse frequency) were depressed in infected rams when compared to control counterparts; none of these endocrine traits were affected for plasma LH.
اظهر المزيد [+] اقل [-]