خيارات البحث
النتائج 1 - 3 من 3
Molecular investigation of Coxiella burnetii infections in aborted sheep in eastern Turkey
2016
Kılıç, A. | Kalender, H. | Koç, O. | Irehan, B. | Berri, Mustapha
Q fever is a zoonotic disease that occurs worldwide and is caused by the obligate intracellular bacterium Coxiella burnetii. The aim of this study was to investigate the presence of C. burnetii infection in aborted sheep in eastern Turkey using PCR. A total of 200 fetuses were collected from aborted sheep belonging to 200 herds in different locations in the eastern part of Turkey. Foetal organ samples such as liver, spleen, lung and stomach were taken and the DNA was purified from two hundred pooled samples. PCR analysis of C. burnetii presence in infected organs was performed, and 4 samples (2%) were found positive. In addition, the pooled organ suspensions were inoculated to embryonated chicken eggs, and PCR analysis of yolk sacs showed C. burnetii DNA in 5 samples (2.5%). This study shows that C. burnetii infection has an important role in sheep abortions in eastern Anatolia region.
اظهر المزيد [+] اقل [-]Molecular investigation of Coxiella burnetii infections in aborted sheep in eastern Turkey
2016
Kiliç, A. | Kalender, H. | Koç, O. | Irehan, B. | Berri, Mustapha | Department of Microbiology, Sivrice Vocational High School ; Firat University | Department of Bioengineering, Faculty of Engineering ; Firat University | Department of Microbiology ; Central Veterinary Control and Research Institute | Infectiologie et Santé Publique (UMR ISP) ; Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT) | Directorate General for Agricultural Research of the Turkish Ministry of Agriculture and Village Affairs : TAGEM/HS/YGAD/12/A07/P02/08
Q fever is a zoonotic disease that occurs worldwide and is caused by the obligate intracellular bacterium Coxiella burnetii. The aim of this study was to investigate the presence of C. burnetii infection in aborted sheep in eastern Turkey using PCR. A total of 200 fetuses were collected from aborted sheep belonging to 200 herds in different locations in the eastern part of Turkey. Foetal organ samples such as liver, spleen, lung and stomach were taken and the DNA was purified from two hundred pooled samples. PCR analysis of C. burnetii presence in infected organs was performed, and 4 samples (2%) were found positive. In addition, the pooled organ suspensions were inoculated to embryonated chicken eggs, and PCR analysis of yolk sacs showed C. burnetii DNA in 5 samples (2.5%). This study shows that C. burnetii infection has an important role in sheep abortions in eastern Anatolia region.
اظهر المزيد [+] اقل [-]Seroprevalence of Chlamydia abortus in camel in the western region of Libya
2016
Rabia Elzlitne | Giuma Elhafi
Objective: The present study was primarily undertaken to determine the seroprevalnace of Chlamidya abortus infection in the Libyan camel (Camelus dromedarius).Materials and methods: Serological tests for C. abortus antibodies were conducted with 245 camels (205 females and 40 males) in different localities in the western region of Libya. Animals varied in age from <1 to 20 years and were sampled randomly from both housed and nomadic herds. From each animal, 10 mL blood sample was collected and sera were separated. Antibodies in the sera against C. abortus were detected using an indirect enzyme-linked immunosorbent assay (ELISA). Results: Results of this study showed that out of the 245 camels tested for anti-chlamydia antibodies, 30 were found positive giving an overall prevalence of 12.25%. The seroprevalence of chlamydiosis was 2 folds higher in females (14.00%) as compared to males (5.00%).Conclusion: The present findings signify the C. abortus as a potential agent to cause abortion in Libyan camel (C. dromedarius). Besides, the persons who handle camels in Libya are at risk of infecting with C. abortus. [J Adv Vet Anim Res 2016; 3(2.000): 178-183]
اظهر المزيد [+] اقل [-]