Objective: The present study was planned to examine the changes in serum protein fractions of lambs with pica symptoms with electrophoresis. Material and Methods: The three study groups were the control group that included healthy lambs, pretreatment and post-treatment groups that included lamb with pica. Lambs with pica symptoms were then treated with a single capsule mineral complex orally and single dose vitamin complex. Serum protein fractions in blood samples were determined with cellulose-acetate electrophoresis.Results: It was determined that in terms of concentration, total protein, albumin and β-globulin, levels did not change, albumin percentage levels increased with pica, however reached control group levels with treatment and α1-Globulin and α2-Globulin levels reduced after treatment. Based on % gr, it was determined that α1-Globulin and β-Globulin did not change and α2-Globulin decreased. ɣ-Globulin level and percentage increased after treatment.Conclusion: Profiles of serum protein fractions may play an important role in the etiology of pica; it was suggested that the identification of serum protein fractions should be considered as a beneficial method in veterinary clinic for the detection, treatment and follow-up of certain diseases and certain nutrition-related conditions.