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Ontogeny, distributions and relative frequencies of the somatostatin-immunoreactive cells in the proventriculus of the chicken embryos with incubation periods
1999
Chang, J.W. | Lee, H.S. (Kyunsan University, Kyungsan (Korea Republic). Department of Biology, Faculty of Natural Science) | Ku, S.K. | Lee, J.H. (Kyungpook National University, Taegu (Korea Republic). Laboratory of Histology, College of Veterinary Medicine)
Ontogeny, distributions and relative frequencies of somatostatin-immunoreactive cells were investigated in the proventriculus of the chicken embryos with incubation periods. Samples were taken from 10 groups(10 days of incubation to hatching) and studied by immunogistochemical methods. The findings were as follows. Somatostatin-immunoreactive cells were observed from 12 days of incubation in the proventricular glands and after that increased with incubation periods. The first observation time of these cells in the epithelium were at 15 days of incubation in the basal portion but in 16 and 17 days of incubation, no immunoreactive cells were observed in the epithelium but after that a few immunoreactive cells were observed in the basal portion and gastric gland regions. The shpaes of these cells were spherical to spindle in the proventricular glands and spherical to round in the epithlium and gastric gland.
اظهر المزيد [+] اقل [-]Localizations of substance P, CGRP and calcium binding proteins in Korean native goat duodenum
1999
Lee, I.S. | Lee, H.S. | Song, S.H. | Yoon, S.T. | Hwang, I.K. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine) | Kang, T.C. | Won, M.H. (Hallym University, Chuncheon (Korea Republic). College of Medicine) | Seo, J.H. (Seoul National University, Seoul (Korea Republic). College of Medicine)
The localization of substance P(SP), calcitonin gene-related peptide(CGRP) and three calcium binding proteins, calbindin D-28k(CB), calretinin(CR) and parvalbumin(PA) was immunohistochemically examined in the myenteric and submucous plexuses of Korean native goat duodenum. In the neurons of myenteric and submucous plexuses of duodenum, immunoreactivities of SP, CGRP and CB were confirmed in both nerve cell bodies and fibers. In contrast, CR immunoreactivity was found only in nerve fibers of myenteric plexuses, while PA immunoreactivity was found only in nerve cell bodies of submucoun plexuses. In the inner circular muscle layer, dense SP-like immunoreactive fibers were prominent but only a few CGRP-like immunoreactivities were observed. Most of SP-and CGRP-like immunoreactive neurons of both plexuses colocalized with CB. This result showed that SP and CGRP may have a important role for the movement of small intestine. The colocalizations of CB with SP or CGRP in myenteric and submucous plexuses suggest that CB may serve neuromodulatory role for SP-and CGRP-immunoreactecd neurons on the movement of intestinal wall.
اظهر المزيد [+] اقل [-]Diethylnitrosamine-induced hepatic tumorigenesis in rats 3. Electron microscopic observaton of liver tissue
1999
Kwak, S.D. | Kim, C.S. | Koh, P.O. | Yang, J.H. | Seo, D.L. (Gyeongsang National University, Chinju (Korea Republic). Institute of Animal Medicine, College of Veterinary Medicine)
The study was designated to investigate the electron microscopic findings following diethylnitrosamine (DEN) treatment in rats. Forty four male (Srague Dawley) rats were continuously given water containing 0.01% DEN for 13 weeks and livers of five rats with more tumor lesions at 16 and 17 weeks after initial treatment were used as EM materials. In transmission electron microscopic findings, most small-sized hepatocytes were active cells containing large mount of organelles, but light (pale staining) hepatocyte among small-sized hepatocytes were injured cells containg disorganized organelles. Tumor cells among small-sized hepatocytes were irregularly arranged and have ;leomorphic nuclei containing electron dense chromatin but the organelles in cytoplasm were swelled. Large-sized hepatocytes were active cells with condensed chromatin but the cytoplasm of these cells were pale due to be injured and dilated organelles. Dark hepatocytes were apoptotic cells with homogenous pyknotic nuclei and cytoplasm, and the cytoplasm of these cells contained dilated smooth endoplasmic reticulum (sER) but these sER were non-vesiculated. Cholangiocarninoma cells were crowed and were pale by far less number of organelles in cytoplasm and nuclei. In scanning electron microscopic findings, the lumens of portal veins, bile canaliculi, bile ductules, bile ducts and sinusoids were dilated and have irregular folded inner surface by protruded parenchyma.
اظهر المزيد [+] اقل [-]Anatomical and histological studies on the developing pancreas of chicken embryos
1999
Ku, S.K. (Dong-Wha Pharmaceutical Industry Company, anyang (Korea Republic). Pharmacol & Toxicol Laboratory, Central Research Laboratories) | Lee, J.H. (Kyungpook National University, Taegu (Korea Republic). Laboratory of Histology, College of Veterinary Medicine) | Lee, H.S. (Kyungsan University, Kyungsan (Korea Republic). Department of Biology, Faculty of Natural science)
Anatomical and histological changes were studied in the dorsal, ventral, third and splenic lobes of the pancreas of the chicken embryos (8 days of incubation, 10 days of incubation to hatching). From 13 days of incubation, all four pancreatic lobes, namely, dorsal, ventral, third and splenic lobes were observed. Histologically, the pancreas of 10-14 days of incubation were consisted of mesenchymal tissue, exocrine acini and pancreatic islets. But mesenchymal tissues were disappeared from 15 days of incubation. The pancreatic ducts were observed from 14 days of incubation. The dark and light typed pancreatic islets were observed in splenic lobe from 13 days of incubation, in the third lobe from 11 days of incubation, and in the dorsall lobe from 13 days of incubation. But no dark typed islets were observed in the ventral lobes.
اظهر المزيد [+] اقل [-]Pharmacokinetics and tissue levels of a sustained release recombinant porcine somatotropin in pigs
1998
Han, S.K. | Ryu, P.D. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine) | Park, S.K. | Chang, B.S. (LG Chemical, Research Park, Seoul (Korea Republic)) | Shim, S.K. (Yonam College of Animal Husbandry and Horticulture, Chonan (Korea Republic))
Histological study on the injury of the seminiferous tubules of the pheasant (Phasianus colchicus) following 60Co gamma-irradiation
1994
Lee, D.M. (Kunsan Junior College, Kunsan (Korea Republic). Department of Radiotechnology)
Histopathological studies on the influence of mast cell in the growth of rat mammary carcinoma, 2; Effect of mast cell mediator on the development of rat mammary carcinoma
1991
Kim, T.H. (Korea Atomic Energy Research Institute, Seoul (Korea Republic). Korea Cancer Center Hospital) | Lee, C.S. (Kyungpook National Univ., Taegu (Korea Republic). Coll. of Veterinary Medicine)
An immunohistochemical study on the gastro-entero-endocrine cells of the pond tortoise (Amyda sinensis)
1990
Kim, J.B. | Lee, J.H. | Lee, N.S. (Kyungpook National University, Taegu (Korea Republic). College of Veterinary Medicine) | Lee, H.S. (Taegu Oriental Medical College, Taegu (Korea Republic). Department of Biology)
Morphological studies on kidney in cyclophosphamide-treated NOD mice
1994
Lee, J.S. | Seong, J.K. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine)
Appearance frequency of spematozoa bearing B-body in semen of Korean native bull and cells bearing F-body in mouse tissues
1993
Kwak, S.D. | Kang, W.H. | Park, S.S. (Gyeongsang National University, Chinju (Korea Republic). College of Veterinary Medicine)