The research was carried out to study the effects of dietary supplementation of Tulasi (Ocimum sanctum) and selenium on ALT and AST enzyme levels in broiler chicken. A total of forty-two broiler chicks of day old divided into six groups of seven each were used for this study. Ocimum sanctum leaf powder (0.25% and 0.5%), organic selenium (0.3 ppm) and their combinations were added to the basal diet. Body weight and feed consumption were recorded at weekly intervals. Blood samples were taken at the end of 6th week for enzymological assay from each treatment. The enzymes assayed were alanine transaminase (ALT) and aspartate transaminase (AST). Dietary supplementation of Ocimum sanctum leaf powder at 0.25%, 0.5% levels and its combination (0.5% level) with selenium (0.3 ppm) reduced the levels of ALT and AST significantly (P<0.05). It was concluded that Tulasi (Ocimum sanctum) has hepato-protective properties.

The twenty adult local male rabbits of mean body weight of 1 kgm and mean age of 6 months were randomly allocated into two groups of ten animals, each group was fed during the period of experiment on a standard diet . The first group was regarded as control and received 3ml of physiological saline orally for a month. The second group was drenched ethanolic extract of Taraxacum officinale (500 mg/kg/day) for one month . Those male were joined with females after treatment for a week to study the effect on fertility and reproductive efficiency. The following results were obtained: The ethanolic extract of Taraxacum officinale caused highly significant increase (p

This study was carried out in Veterinary Medicine College / University of Basrah to investigate the effect of Bisphenol A on thyroid, liver and testicular functions. A total of 24 adult male rats were randomly divided into four equal groups, six animals in each group. Animal of group (1) served as control and received a daily oral administration of corn oil throughout the experimental protocol. Animals of group 2, 3 and 4 were administered orally 50,100 and 200 mg/kg body weight of BPA respectively dissolved in corn oil, the experiment extended for 30 days. The results of the present study showed a significant decrease in serum thyroxin (T4) concentration and a significant increase in serum aspartate aminotransferase ( AST) and alanine aminotransferase ( ALT) concentration in bisphenol A treated groups. A significant decrease in serum testosterone and LH concentrations in all BPA-treated groups compared with control. On other hand non significant decrease in serum concentrations of FSH were observed in BPA-treated groups compared with control. A significant decrease in epididymal sperm count and sperms motility in all BPA treated groups. However no significant differences were noted in sperms viability between all BPA treatment groups and control. Histopathological changes were found in thyroid glands of male rats with different doses of BPA, also central vein dilation, enlarged nuclei, vacuolation of hepatocytes were observed in the liver of BPA treated groups and different degrees of histological changes include depression of spermatogenesis, decrease of leydig cells in dose dependent manner were found in testicular tissues of BPA treated groups.

Short- and long-term exposure to sub-lethal copper-sulfate concentrations studied in freshwater fish (Carassius auratus). The blood parameters, such as the content of hemoglobin-Hb and the red blood cells-RBC, were examined. Alanine transaminase-ALT and aspartate aminotransferase-AST levels in the serum were also measured. The 96-hour copper sulfate median lethal concentration-LC50 for the fish (n= 100) was calculated using different concentrations (1–10 mg/l). Two hundred and forty fish were put in 90 l-glass aquariums (10 in each aquarium) (180 fish in the experimental treatments) and (60 fish in the control treatments). Two groups of fish were formed. The first group was subjected to different concentrations of copper-sulfate for one week: 1 mg/l (0.58 copper sulfate LC50), 2 mg/l (1.15 copper sulfate LC50), and 3 mg/l (1.72 copper sulfate LC50). The second fish group received the same copper-sulfate concentrations but for four weeks. The treatment with zero copper-sulfate LC50 served as the control. Three duplicates of each treatment were achieved (A total of 340 fish were used). The results demonstrated that the content of RBC and Hb, as well as the level of the enzymes (AST and ALT), increased significantly with increasing copper-sulfate concentration in both the short- and long-term exposed fish. The current study's findings suggest that sub-lethal copper-sulfate concentrations are responsible for bloody changes and liver dysfunction in fish. The environment's copper contamination can be monitored using these parameters. 

Objective, The purpose of this experiment is to study the circadian rhythm of common liver function tests. Materials and Methods, Ten adult sheep (male and female) were divided into two groups, T1 (male) and T2 (fe-male), and placed in two separate rooms. Serum was isolated from T1 and T2 blood samples taken at 7, 12 A.M. and 12 P.M hours, respectively.Results, Serum alanine aminotransferase (ALT) and creatinine levels in both groups increased significantly during daylight hours (12:00). The aspartate aminotransferase (AST) showed a different value in a male that increased significantly at 12 A.M. but in a fe-male that was elevated at 7 A.M. as compared with other times. Serum urea concentra-tion during the night hours was expressed at a high level in both males and females as compared to the concentration during the day hours. In addition, the results show differences in variation between males and females. There is a significant increase in ALT, urea and creatinine in females (T2) as compared with males (T1) during the day and night hours. Furthermore, at 12 A.M. and 12 P.M., males had significantly higher AST levels than females Conclusion, liver functions cause variations between day and night hours as well as between males and females because of metabolism and activities. Comprehensive study is required to reduce and overlap this stress.

This study was carried out to find whether phenidone (1-phenyl-3-pyrazolidinone), a cyclooxygenase as well as a lipoxygenase inhibitor, exhibits the preventive effect on carbon tetrachloride (CCl₄)-induced acute liver injury in rats. Rats were pretreated with phenidone at a dose of 50 or 200 mg/kg (p.o.) once daily for 3 consecutive days before CCl₄ administration. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured. Malondialdehyde (MDA) production was determined as an index of lipid peroxidation in the liver and serum. The histopathological changes in the liver were also examined in each group. The reduction in body weights was significantly inhibited in the phenidone-treated group than in the CCl₄ control group. Significant increase in the relative liver weights of the phenidone-treated groups was observed compared with either the vehicle or CCl₄ groups. Elevation of serum AST and ALT activities occurred after CCl₄ treatment was significantly attenuated by the pretreatment with phenidone. The elevation of MDA levels in liver and serum were completely inhibited in phenidone-treated groups. The protective effects on phenidone-treated groups were confirmed histopathologically. These results suggest that phenidone may be a useful protector through modulation of hepatic inflammation in CCl₄-induced acute liver injury.

Objective: This study aims to investigate the hepatoprotective, anti-inflammatory, and wound-healing potentials of spirulina utilizing a rat model. Materials and Methods: Carbon tetrachloride (CCl4) was used to induce hepatotoxicity, while carrageenan was employed to induce hind paw inflammation. The wound healing capability was assessed by making a 6 mm round wound with a biopsy punch on the dorsal interscapular area of each rat. The animals were fed a diet mixed with spirulina at dosages of 250 and 500 mg.kg−1bwt. Paw thickness measurements were taken at 1, 3, and 6 h after carrageenan injection. Results: Intraperitoneal injection of CCl4 leads to hepatotoxicity, as evidenced by significantly elevated serum levels of alanine aminotransferase and aspartate aminotransferase. Treatment with spirulina markedly reduced these biochemical markers. CCl4-induced hepatic cellular necrosis, central vein congestion, and steatosis were notably improved following spirulina administration. Additionally, spirulina treatment diminished paw edema and shortened wound closure time in a dose-dependent manner. Histopathological analysis of the inflamed paw revealed massive infiltration of inflammatory cells and thickening of the epidermis, both of which showed significant improvement with spirulina treatment. The anti-inflammatory and wound-healing effects of spir¬ulina were comparable to those of indomethacin, an established anti-inflammatory drug. Conclusion: Our findings demonstrate that Spirulina platensis possesses beneficial properties in counteracting hepatotoxicity and inflammation. Additionally, it exhibited significant wound-healing effects in rat models. These results reinforce the potential health advantages of spirulina as an effective functional food. [J Adv Vet Anim Res 2025; 12(1.000): 303-311]

Objective: The objective of this study was to evaluate some hematological parameters in commercial layers inoculated with two virulent Pasteurella multocida serotypes. Materials and Methods: A total of 84 twenty-week-old black Harco layers were randomly assigned to seven groups (A, B, C, D, E, F and G) with 12 birds per group. 1mLof live attenuated fowl cholera (FC) vaccine was administered subcutaneously at 24 weeks of age to groups A and B, emulsified inactivated (killed) FC vaccine was administered dosed at 0.5 mL per bird subcutaneously at 24 weeks of age to groups C and D, groups E and F were not vaccinated, while group G served as control. Groups A, C and E were inoculated with P. multocida serotype A:1 and groups B, D and F were inoculated with P. multocida serotype A:3. Using McFarland Standard, each bird received a dose of 0.5 mL (0.1 mL intranasally and 0.4 mL intramuscularly) containing 4.5 x 108 cfu/bird. Results: For PCV (P≤0.2692 and P≤0.7643) and HB (P≤0.2806 and P≤0.7266) on day 2 and 10 post inoculation, there was no significant difference between the vaccinated, non-vaccinated groups and control group G. However, there was a highly significant difference P≤0.05 in the mean concentrations of ALP between the control group G (67.67±1.453 u/l) vaccinated groups A (80.33±4.98 u/l), B (81.33±2.60 u/l), C (75±6.35 u/l), and D (84±5.132 u/l) and unvaccinated groups E (104±1.528 u/l ), and F (78 ±3.512 u/l) post inoculation. Conclusion The PCV significantly decrease P≤0.05 in layers vaccinated and inoculated with P. multocida but increase in unvaccinated layers inoculated P. multocida. The mean serum ALP concentration significantly increase P≤0.05 in unvaccinated layers inoculated with P. multocida when compared to layers vaccinated and inoculated with P. multocida. [J Adv Vet Anim Res 2017; 4(3.000): 234-240]

Objective: This study was conducted to assess the effect of dietary Moringa oleifera leaf (MOL) feed supplementation on serum biochemical parameters of broilers challenged with very virulent infectious bursal disease virus (vvIBDV). Materials and methods: Two hundred and forty day-old Ross 308 hybrid broiler chicks were randomly assigned into four groups (A, B, C and D) of 60 chicks each and raised in deep litter housing. Broiler starter (BS) and broiler finisher (BF) mash were formulated each with 5% MOL included as part of the feed ingredient for broilers in groups A and B while BS and BF for broilers in groups C and D were formulated without MOL. Broilers in groups A, B and C were challenged intraocularly at 35 days of age with with 0.05 mL of a live vvIBDV, while those in group D served as control. Blood was collected from 10 broilers in each group via the wing vein at 35, 38 and 42 days of age to determine their serum biochemical profile. Results: The level of melondialdehyde (MDA) was observed to significantly decrease in groups A and C. There was a significant decrease in the level of AST in group A, B, C and D. The values of ALT significantly decreased in group A, B, C and D. Conclusion: Supplementing broilers feed with MOL neither protect the liver from damage nor prevent lipid peroxidation. [J Adv Vet Anim Res 2018; 5(2.000): 155-165]

O objetivo do trabalho foi avaliar a influência do exercício físico (provas de marcha) sobre os valores séricos de AST e CK e valores plasmáticos de lactato em equinos da raça Mangalarga Marchador criados no estado do Espírito Santo. Amostras de soro e plasma foram obtidas de 15 equinos em quarto diferentes momentos: repouso (T0) e com 5 minutos (T1), 30 minutos (T2) e 2 horas (T3) após o término do exercício. Foram registrados valores de lactato plasmático de 1,02 ± 0,41 mmol/L, 2,73 ± 2,43 mmol/L, 1,89 ± 1,24 mmol/L e 1,31 ± 0,60 mmol/L, respectivamente nos momentos T0, T1, T2 e T3. Na análise de AST, os resultados registrados nos momentos T0, T1, T2 e T3 foram, respectivamente, de 189,3 ± 56,0 UI/L, 223,9 ± 53,5 UI/L, 186,8 ± 25,8 UI/L e 193,9 ± 44,7 UI/L. Finalmente, os valores séricos de CK foram de 113,4 ± 56,3 UI/l, 144.1 ± 70,9 UI/L, 143,0 ± 81,0 UI/L e 173,1 ± 128,0 UI/L, respectivamente nos momentos T0, T1, T2 and T3. A análise dos resultados demonstrou que a marcha influenciou de forma significativa o lactato plasmático, porém não influenciou a atividade sérica de AST e CK, sugerindo que os equinos usados encontravam-se condicionados ao exercício físico imposto.