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Zoonotic diseases diagnosed from Jan 2016 to Aug 2017 in Regional Veterinary Laboratories, Department of Veterinary Services, Malaysia
2018
Zulkifli A. | Santhi M. | Marzuki Z. | Rohana A. B. | Norasyikin A. | Tariq J. | Chandrawathani P. | Saira Banu M. R. | Faizah Hanim M. S.
Infectious diseases of livestockare a major threat to global animal health and welfare and their effective control is crucialfor agronomic health, for safeguarding and securing national and international food supplies and for alleviating rural povertyin developing countries. Some devastating livestock diseases are endemic in many parts of the world and threats from old and new pathogens continue to emerge, with changes to global climate, agricultural practices and demography presenting conditions that are especially favourable for the spread of arthropod-borne diseases into new geographical areas. Zoonotic infections that are transmissible either directly or indirectly between animals and humans are on the increase and pose significant additional threats to human health and the current pandemic status of new influenza A (H1N1) is a topical example of the challenge presented by zoonotic viruses (Tomley and Shirley, 2009). Malaysia, being one of the members of the World Organisation forAnimal Health (OIE) which is responsible for setting standards for control of animal diseases. For year 2017, the list included 116 animal diseases, infections and infestations, many of which are zoonotic in nature. As such, this paper discusses the commonzoonotic infections diagnosed in the five Regional Veterinary Laboratories which are spread across the country and entrustedto carry out diagnostic tests to aid in the treatment and control of animal diseases. A total of almost half a million samples weretested comprising more than a million tests to help the Department of Veterinary Services control and eradicate economically important diseases to safeguard the animal population. Of these, zoonotic diseases comprise a small but significant entity which needs careful attention (Chandrawathani et al., 2017) Dora Tan (1981) reported that among the many zoonotic diseases prevalent in Malaysia, are leptospirosis, rabies, influenza, Japanese encephalitis, toxoplasmosis,ornithosis, Q fever and monkeypox which have been investigated at the lnstitute for Medical Research, Kuala Lumpur. The regional laboratories have full capability to conduct tests to confirm parasitic, viral and bacterial infections except for rabies andavian influenza, which was diagnosed in the Veterinary Research Institute. However, preliminary tests for avian influenza wascarried out in regional laboratories.
اظهر المزيد [+] اقل [-]Whole genome sequence of Brucella melitensisl local isolate from an infected goat in Malaysia
2016
Mohd Mokhtar Arshad | Ramlan Mohamed | Shuhaila Mat Sharani | Hardy Abu Daud | Omer Khazaal Sallou | Mohd Azam Khan Goriman Khan | Hirzahida Mohd. Padil
Brucellosis in goats is mainly caused by the bacterium Brucellamelitensis, which is one of the most important pathogenic species in the world. In Malaysia, the annual prevalence data of brucellosis was recorded in goats and the control strategy of the disease basedon test and cull of infected animals. This strategy has caused huge economic losses to farmers and government alike. Therefore, whole genome sequencing of B. melitensis local strain is essential forimproving the current vaccine. B. melitensis strain VRI 6530/11 wasobtained from veterinary research institute biobank, Ipoh. The strain was submitted for classical identification procedures and the total genomic DNA was extracted by using DNeasy blood and tissue kit(QIAGEN). The concentration and purity of DNA were determined by using agarose gel electrophoresis and spectrophotometer (DNA/RNA) assay respectively. The genome was sequenced by using IlluminaHiSeq platform with insert size ~200 bp. A total of 1.0 Gb data was generated from the sample. More than 95% of sequencing data was retained in the sample after quality filtering, this indicatethe sequencing reads are of high quality. Final assembly had 33 scaffolds with total size ~3.28 Mb, 44 contigs, GC content is 57.25%, N50 is 293,291. A total of 3,238 protein coding genes, 48 tRNAs and 3rRNAs were predicted and over 87% of the genes were functionally annotated. Genome sequencing of a local B. melitensis strain is the first of its kind in Malaysia and work from this study can contribute towards the development of a new effective vaccine for the control ofthe disease in the country.
اظهر المزيد [+] اقل [-]Monitoring of Brucella sp., Coxiella burnetii and aflatoxin M1 in goat milk from Johor
2018
Sarol K. | Putri Nur Atifi M. N. | Saudah A. | Ketty, G. S. L. | Khairunnisak M. | Faridah I. | Nursyuhada M. R.
Forty-nine fresh goat’s milk samples produced by local farmers and sold in market for public consumption as well as raw goat milk in Johor, Malaysia were analysed for total plate count(TPC) , E. coli, Coliform, Brucella melitensis, Brucella abortus,Coxiella burnetii as well as aflatoxin M1 (AFM1) content, as measures for food safety. The mean counts per ml for TPC were 4.90 x 105, 6.50 x 105, 1.60 x 105 and 1.48 x 106 for pasteurised, unpasteurised and unknown (status of pasteurisation) milk sold in the market as well as the raw milk from milkcollection center (MCC), respectively. Among pasteurised samples, only one had TPC count higher than the permitted level whereas the rest were all within the permitted level. The mean counts per ml for E. coli were <1.00 x 102 for pasteurised and unknown milkwhereas 1.67 x 101 for unpasteurised and 1.18 x 102 for raw milk. The mean counts per ml for coliform were 9.53 x 103, 9.76 x103, 1.20 x 102 and 1.16 x 104 for pasteurised, unpasteurised, unknown milk and raw milk, respectively. Overall, no significantdifferences on the bacterial counts in both pasteurised and unpasteurised milk. All milk samples were negative of B. melitensis and B. abortus, but one unknown sample fromthe market and two raw samples from MCC were positive of C. burnetii through the ELISA test. The unknown sample from the market showed the presence of C. burnetii when further analysed microscopically. Meanwhile, no sample exceeded the permitted level of AFM1 in milk.
اظهر المزيد [+] اقل [-]Isolation and molecular characterization of Brucella abortus and Brucella melitensis from samples received by the Regional Veterinary Laboratory, Bukit Tengah, Malaysia
2017
Thenamutha M. | Zakiah M. D. | Azizul O. | Maswati M. A.
A study was carried out to report the phylogenetic analysis of Brucella abortus and Brucella melitensisby using molecular techniques from samples submitted to the Regional Veterinary Laboratory, Bukit Tengah.In this study, identification and genetic characterization of Brucella isolated samples using molecular analysis based on IS711 sequence between localisolates and foreign countries accesses in GenBank was done successfully. A total of 31 samples were isolated for Brucella species and then were amplified byPCR, directly sequenced and compared genetically to published sequences which were obtained from GenBank. The most common Brucella species that was found in both bovine (76.5%) and caprine (85.7%) through diagnostic samples in Regional Veterinary Laboratory, Bukit Tengah, was Brucella melitensis. PCR and sequencing were confirmed positive with 76.5% for Brucella melitensis, 23.5% for Brucella abortus and 23.5% for mixed infectionfrom the total of 17 bovine samples. In caprine, the detection of Brucella melintesis and Brucella abortus showed 85.7% and 21.4% respectively meanwhile total mixedinfection showed 21.4%. These clustering between local isolates of Brucella melitensis were phylogenetically related to other Asian countries such as Singapore,Yemen and Saudi Arabia. The Neighbour Joining Analysis clustered the Brucella abortus local isolates for both bovine and caprine were most closely related to India,Iran, Italy and USA. Interestingly, all the isolates within Malaysia have a close relationship (>95%) with the low level of genetic diversity. When local isolates arecompared to GenBank data, it gives an indication on the possible sources of these infections. Eventually, it will improve the import and export policies to controlbrucellosis in Malaysia.
اظهر المزيد [+] اقل [-]Development of an in-house Rose Bengal plate test for diagnosis of Brucellosis in goat
2016
Mohamed Ariff O. | Siti Khairani Bejo | Asinamai Athliamai Bitrus | Sani M. Y. | Zakaria Zunita
Brucellosis, caused by Brucella melitensis, is a significantproblem for both public and animal health worldwide. The Rose Bengal plate test (RBPT) antigen from Brucella melitensis local isolates were developed in this study. The performance of the assay wasinvestigated using serum samples collected from goats. A total of 1063 serum samples obtained from goats were examined for thepresence of antibodies against Brucella by in-house RBPT (LRBPT), commercial RBPT (Veterinary Laboratory Agency – VLA, UK) and Compliment Fixation test (CFT). The sensitivity and specificity wascalculated using CFT as the gold standard. Out of 1063 goats sera analyzed 364 (34.24%), 335 (31.51%), and 373 (35.08%) were found to be positive by LRBPT, commercial RBPT and CFT, respectively. The sensitivity calculated for the LRBPT, was 90.1% compared to commercial RBPT 85.0%. However, the specificity of the LRBPT was lower (95.9%), than the commercial RBPT (97.4%). Furthermorethe LRBPT has better value of NPV (94.7%) than commercial RBPT NPV(92.3%). While the PPV, of the commercial RBPT is higher (94.6%) than LRBPT (92.3%) respectively. High sensitive and low cost LRBPT compared to cRBPT B. melitensis RBPT test was successfully developed in this present study. Therefore it was concluded that this diagnostic test kit can complement and replace the availablecommercial RBPT which is relatively more expensive and less sensitive in detection of brucellosis in goats. It could also be used for epidemiological surveillance of caprine brucellosis in Malaysia.
اظهر المزيد [+] اقل [-]Critical diseases diagnosed in small ruminants in Perak, Malaysia: the role of management in disease outbreaks in the hot tropics
2013
Quaza Nizamuddin N. M. | Muhamad Hazmi Y. | Erwanas A. | Norazura A. H. | Ainani A. | Zawida Z. | Azfarina Z. | Mohamad Bohari J. | Ramlan M. | Chandrawathani P. | Abdul Sukor S. | Norhafiza H. | Roshalinda D.
The small ruminant industry in Malaysia is plagued by several significant problems related to health and management. Due to the hot, wet tropical climate, parasitic, bacterial and viral diseases are rampant and conveniently spread and enhanced by poor management. The Veterinary Research Institute has initiated a working group to
identify and control critical diseases in small ruminants as an educational cum advisory programme for farmers on the lower economic rung so that productivity and profits may be heightened. Farms are attended to base on the complaint of high mortality and morbidity, whereby a complete and holistic approach to disease control is taken. All the farms sampled were in the vicinity of the laboratory
that is within 150 km radius. Samples collected were faeces, blood (serum and whole blood) and nasal swabs as well as water and feed samples. A questionnaire on the management practices as well as
history of the farm was recorded. Tests on all samples were conducted according to routine procedures. Results from this study
on 7 farms involving 175 goats and sheep revealed that the common parasitic diseases encountered are helminthiasis, coccidiosis and blood protozoans. More than 80% of the animals showed strongyle and coccidial infections. The prominent bacterial infections are Caseous Lymphadenitis (34.3%), Brucellosis (Brucella melitensis), Leptospirosis (1.7%), Melliodosis and QFever whereas the viral diseases are Caprine Arthiritis Encephalitis (1.1%) and Blue Tongue (15.4%). Early detection for these diseases is critical so that production losses can be minimised. Poor nutrition and hygiene is a contributing factor for the high incidence of disease in small
ruminants. Farmer awareness on latest farming methods and services provided also contributes to general wellbeing of animals. One of the outcomes of this programme is to create awareness and getting farmers to be independent and confident in problem solving with regards to management and disease in their herds.
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