خيارات البحث
النتائج 1 - 3 من 3
Expression and antigenicity characterization for truncated capsid protein of porcine circovirus type 2
2011
Lou, Zhongzi | Li, Xuerui | Li, Zhiyong | Yin, Xiangping | Li, Baoyu | Lan, Xi | Yang, Bin | Zhang, Yun | Liu, Jixing
Three pairs of specific primers were designed to amplify F2-1, F2-2, and XF2-2 truncated capsid protein genes of porcine circovirus type 2 (PCV-2). Amplified sequences were subcloned to pET-32a(+) vectors and expressed in Rosetta (DE3) Escherichia coli by induction of isopropy-β-D-thiogalactoside (IPTG). All of the fusion proteins had positive reactions to PCV-2 antiserum and His-XF2-2 showed the best reactivity. Proteins were used to immunize BALB/c mice to produce monoclonal antibodies (mAbs), and 7 mAbs were selected. Capsid protein N-terminal parts 55 to 96 amino acid (aa), 97 to 141 aa, and 143 to 211 aa were confirmed as binding regions of the 7 mAbs. Reactivity between His-XF2-2 and the 7 mAbs was detected, FmAb-8 showed the best reactivity. The dominant B-cell epitope was located at 97 to 141 aa. The PEPSCAN indicated that the P122–136 peptide contained the dominant B-cell epitope.
اظهر المزيد [+] اقل [-]Molecular evolution of porcine reproductive and respiratory syndrome virus in Guangxi of China from 2012 to 2015
2019
Song, M. (Qingdao Agricultural University, Qingdao (China). College of Veterinary Medicine) | Zhang, Q. | Shan, H. | Yu, B. | Xiong, Y. | Li, J.
Real-time PCR assay for rapid differentiation of env-based genotypes of feline leukemia virus
2019
Nakagawa, S. (Iwate University, Morioka, Iwate (Japan). Faculty of Agriculture, Cooperative Department of Veterinary Medicine, Laboratory of Veterinary Microbiology) | Kitamura, Y. | Naito, I. | Kaneda, M. | Chiba, Y. | Shimamura, S. | Yamasaki, M. | Hikono, H. | Murakami, K.
